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[173995_at]

Description AU112477 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C33H5.18 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AU112477 /WB_GENE_ID=C33H5.18 /WP=CE04161 /CHR=4 /FEA=Genomic Cluster /DEF=CDP-diacylglycerol synthase (ST.LOUIS) SW:P53439 protein_id:AAA82275.1; Caenorhabditis elegans cDNA clone:yk743c8 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 28      4grp_highest_8_8 > 50      4grp_high_8_8 > 32      6grp_highest_nonorm > 0      6grp_total > 18      4grp_total > 47      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 920
1 2 3 4 range
A 2228.1
(3)
P 		1419.4
(-2.8)
P 		1511.4
(-2.1)
P 		1391.5
(-3)
P 		837
B 2216
(3)
P 		1540.4
(-3)
P 		1554.8
(-2.9)
P 		1547.8
(-2.9)
P 		676
C 2311.7
(3)
P 		1518.7
(-2.2)
P 		1522.9
(-2.2)
P 		1397
(-3)
P 		915
avr 2251.9
(3) 		1492.8
(-2.6) 		1529.7
(-2.4) 		1445.4
(-3) 		807

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1205
1 2 3 4 5 6 range
A 2207.4
(3)
P 		1693.9
(-0.3)
P 		1505.8
(-1.5)
P 		1278.1
(-3)
P 		1532.5
(-1.4)
P 		1467.1
(-1.8)
P 		929
B 2227.2
(3)
P 		1614.5
(-0.1)
P 		1022.3
(-3)
P 		1581.4
(-0.2)
P 		1384.5
(-1.2)
P 		1441.1
(-0.9)
P 		1205
C 2053
(3)
P 		1517.1
(-1.2)
P 		1493.2
(-1.4)
P 		1294.7
(-3)
P 		1360.1
(-2.5)
P 		1622.6
(-0.4)
P 		758
avr 2162.5
(3) 		1608.5
(-1) 		1340.4
(-3) 		1384.7
(-2.7) 		1425.7
(-2.4) 		1510.3
(-1.8) 		822
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 608
1 2 3 4 range
A 811.3
(3)
P 		308.1
(-2)
P 		395.1
(-1.1)
P 		203.7
(-3)
P 		608
B 706.7
(3)
P 		380.5
(-2.2)
P 		360.3
(-2.5)
P 		329.8
(-3)
P 		377
C 765.3
(3)
P 		436.6
(-0.7)
P 		530.8
(0.3)
P 		235.6
(-3)
P 		530
avr 761.1
(3) 		375.1
(-1.6) 		428.7
(-1) 		256.4
(-3) 		505

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 527
1 2 3 4 5 6 range
A 406.9
(-1.2)
P 		602.4
(3)
P 		327.9
(-2.9)
P 		324.2
(-3)
P 		462.5
(-0)
P 		357.2
(-2.3)
P 		278
B 775
(3)
P 		537.4
(0.3)
P 		248.2
(-3)
P 		418.9
(-1.1)
P 		562.5
(0.6)
P 		537.4
(0.3)
P 		527
C 761
(3)
P 		462.9
(-0.6)
P 		380
(-1.6)
P 		260.4
(-3)
P 		440.1
(-0.8)
P 		543.2
(0.4)
P 		501
avr 647.6
(3) 		534.2
(0.9) 		318.7
(-3) 		334.5
(-2.7) 		488.4
(0.1) 		479.3
(-0.1) 		329
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173995_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24820
Sequence Type Exemplar sequence
Representative Public ID AU112477 NCBI
Target Description AU112477 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C33H5.18 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AU112477 /WB_GENE_ID=C33H5.18 /WP=CE04161 /CHR=4 /FEA=Genomic Cluster /DEF=CDP-diacylglycerol synthase (ST.LOUIS) SW:P53439 protein_id:AAA82275.1; Caenorhabditis elegans cDNA clone:yk743c8 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068897(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068897 NCBI Caenorhabditis elegans C33H5.18b (C33H5.18) mRNA, complete cds. 11/11 None
C33H5.18b ENSEMBL cdna:known chromosome:CEL140:IV:7812753:7815716:-1 gene:C33H5.18 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
3565046 2 1 NM_068897 173995_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7812974-7815723(-) 100.0 100.0
Public Domain and Genome References
Ensembl C33H5.18
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK84502 Hypothetical protein C33H5.18b [Caenorhabditis elegans] ref|NP_501298.1| C33H5.18b [Caenorhabditis elegans] 0.0
blast AAA82275 Hypothetical protein C33H5.18a [Caenorhabditis elegans] ref|NP_501297.1| C33H5.18a [Caenorhabditis elegans] sp|P53439|CDS1_CAEEL Putative phosphatidate cytidylyltransferase (CDP-diglyceride synthetase) (CDP-diglyceride pyrophosphorylase) (CDP-diacylglycerol synthase) (CDP-DAG synthase) (CDP-DG synthetase) 0.0
blast CAE70893 Hypothetical protein CBG17684 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000374 EMBL-EBI Phosphatidate cytidylyltransferase 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501298.1 8 95-117,121-143,156-178,188-210,223-245,250-272,285-307,362-384
C33H5.18b 8 95-117,121-143,156-178,188-210,223-245,250-272,285-307,362-384
Sequence
Target Sequence 
>C. ELEGANS:173995_AT
actggattaatctagagatctgtaactgcatcatttcattccatatgcatctcaaaaatg
gaatttctctttgtctagtcattcctttttcccattcactaccattctctgtcctgttcc
tacgctttaaccagtcctgtttcatttttttgttcaaatttccaccaacttcttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTGGATTAATCTAGAGATCTGTAA 83 97 1367 Antisense
GAGATCTGTAACTGCATCATTTCAT 173 395 1381 Antisense
TCTGTAACTGCATCATTTCATTCCA 246 607 1385 Antisense
ATCATTTCATTCCATATGCATCTCA 223 31 1396 Antisense
TTCATTCCATATGCATCTCAAAAAT 300 689 1401 Antisense
AAATGGAATTTCTCTTTGTCTAGTC 528 119 1422 Antisense
AATTTCTCTTTGTCTAGTCATTCCT 444 175 1428 Antisense
CTACGCTTTAACCAGTCCTGTTTCA 380 223 1486 Antisense
ACGCTTTAACCAGTCCTGTTTCATT 635 91 1488 Antisense
GCTTTAACCAGTCCTGTTTCATTTT 158 305 1490 Antisense
TGTTCAAATTTCCACCAACTTCTTC 424 559 1517 Antisense
Sequence Source Affymetrix Proprietary Database