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[173989_s_at] protease

Description CE21A4 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F21F8.7 /5_PRIME_EXT_DB=WormBase Gene ID /GB=M89319 /WB_GENE_ID=F21F8.7 /WP=CE09542 /CHR=5 /FEA=Genomic Cluster /GEN=asp-6 /DEF=protease; CEL21A4 Chris Martin sorted cDNA library Caenorhabditis elegans cDNA clone cm21a4 5prime similar to pepsinogen A homologous peptide, mRNA sequence.
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 11      4grp_highest_8_8 > 33      4grp_high_8_8 > 51      6grp_highest_nonorm > 09      6grp_total > 32      4grp_total > 61      6grp_highest_sub > De      6grp_cgc > 70      6grp_high1_sub > De      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23443
1 2 3 4 range
A 27404.1
(3)
P 		25673.4
(2.6)
P 		25599.1
(2.5)
P 		4317.9
(-3)
P 		23086
B 26715.9
(3)
P 		22697.5
(1.9)
P 		23858.9
(2.2)
P 		5660.6
(-3)
P 		21055
C 24167.4
(3)
P 		20658.9
(2)
P 		21378.3
(2.2)
P 		3961.1
(-3)
P 		20206
avr 26095.8
(3) 		23009.9
(2.1) 		23612.1
(2.3) 		4646.5
(-3) 		21449

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12601
1 2 3 4 5 6 range
A 27011.2
(3)
P 		23425.6
(0.8)
P 		25567.5
(2.1)
P 		21766.7
(-0.2)
P 		19458.3
(-1.5)
P 		17038.1
(-3)
P 		9973
B 23547.2
(2.9)
P 		21927.1
(1.8)
P 		23705.2
(3)
P 		20892.9
(1.1)
P 		18928.5
(-0.2)
P 		14806.8
(-3)
P 		8898
C 23973.3
(3)
P 		21421.1
(1.4)
P 		23078.1
(2.4)
P 		20869
(1.1)
P 		23516.3
(2.7)
P 		14410.7
(-3)
P 		9563
avr 24843.9
(3) 		22257.9
(1.4) 		24116.9
(2.5) 		21176.2
(0.7) 		20634.4
(0.3) 		15418.5
(-3) 		9425
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9346
1 2 3 4 range
A 9977.9
(3)
P 		5573
(0.2)
P 		6692.1
(0.9)
P 		632
(-3)
P 		9346
B 8519.5
(3)
P 		5605.8
(0.6)
P 		5528.7
(0.5)
P 		1206.3
(-3)
P 		7313
C 8000.1
(3)
P 		5939.6
(1.3)
P 		7451
(2.6)
P 		667.9
(-3)
P 		7332
avr 8832.5
(3) 		5706.1
(0.7) 		6557.3
(1.3) 		835.4
(-3) 		7997

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4738
1 2 3 4 5 6 range
A 4979.3
(-1.8)
P 		8331.3
(3)
P 		5568.1
(-1)
P 		5520.5
(-1)
P 		5871.7
(-0.5)
P 		4148.2
(-3)
P 		4183
B 8193.3
(3)
P 		7298.6
(1)
P 		5756.3
(-2.5)
P 		5533.8
(-3)
P 		7690.4
(1.9)
P 		5521.4
(-3)
P 		2672
C 8885.9
(3)
P 		6536.2
(-0)
P 		5873.8
(-0.9)
P 		4196.8
(-3)
P 		7609.5
(1.4)
P 		4824.1
(-2.2)
P 		4689
avr 7352.8
(2.9) 		7388.7
(3) 		5732.7
(-0.9) 		5083.7
(-2.4) 		7057.2
(2.2) 		4831.2
(-3) 		2558
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173989_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25252
Sequence Type Exemplar sequence
Representative Public ID CE21A4 NCBI
Target Description CE21A4 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F21F8.7 /5_PRIME_EXT_DB=WormBase Gene ID /GB=M89319 /WB_GENE_ID=F21F8.7 /WP=CE09542 /CHR=5 /FEA=Genomic Cluster /GEN=asp-6 /DEF=protease; CEL21A4 Chris Martin sorted cDNA library Caenorhabditis elegans cDNA clone cm21a4 5prime similar to pepsinogen A homologous peptide, mRNA sequence.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072732(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072732 NCBI Caenorhabditis elegans F21F8.7 (protease) mRNA, complete cds. 11/11 A 
SNAP00000008545 ENSEMBL cdna:SNAP chromosome:CEL140:V:8270204:8271525:-1 11/11 None
GENEFINDER00000008561 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8270204:8271525:-1 11/11 None
F21F8.7.1 ENSEMBL cdna:known chromosome:CEL140:V:8270009:8271527:-1 gene:F21F8.7 11/11 None
F21F8.7.2 ENSEMBL cdna:known chromosome:CEL140:V:8270147:8271529:-1 gene:F21F8.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8270176-8271527(-) 100.0 100.0
Public Domain and Genome References
Gene Title protease
Gene Symbol asp-6
Ensembl F21F8.7
Entrez Gene 179209 Entrez gene
SwissProt O01530 EMBL-EBI
WormBase CE09542 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4190 aspartic-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
4194 pepsin A activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC47996 Aspartyl protease protein 6 [Caenorhabditis elegans] ref|NP_505133.1| F21F8.7 [Caenorhabditis elegans] 0.0
blast CAE66088 Hypothetical protein CBG11305 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 4.5E-74
Sequence
Target Sequence 
>C. ELEGANS:173989_S_AT
aacgccatcaaccaaggaatccttgaccagccactcttctcagtctggcttgagcacaga
ggagccgccaacaatgttggaggaggagtcttcacctacggagccattgacaccaccaac
tgcggagcccttgttgcctaccaaccactttcctctgccacctactatcaattcaaggct
gccggattcaaactcggatcatactcaaacaccaagaccgttgatgttatctctgacacc
ggaacttccttcctcggaggaccacaatctgttgttgacggactcgccaaggccgctgga
gccacttatgatgactttaacgaggtctacttcatcgactgcgctgcccagccaggaact
cttgatattaccattggaaccaacacttattccatccagccagtcaactacatcgttgat
gccggaaacggtcaatgccttttcgctgccttcccattcgatttcggaggattcggacca
tcatggattcttggagatccattcattcgtcaatactgcaacatttacgatattggaaac
aagagaatgggattcgctccatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGCCATCAACCAAGGAATCCTTG 472 145 608 Antisense
ACTCTTCTCAGTCTGGCTTGAGCAC 273 101 640 Antisense
GGCTTGAGCACAGAGGAGCCGCCAA 706 531 654 Antisense
CACAGAGGAGCCGCCAACAATGTTG 605 193 662 Antisense
CTACTATCAATTCAAGGCTGCCGGA 505 225 769 Antisense
GGCTGCCGGATTCAAACTCGGATCA 11 541 784 Antisense
GATCATACTCAAACACCAAGACCGT 606 419 804 Antisense
GACCGTTGATGTTATCTCTGACACC 85 383 823 Antisense
ATGTTATCTCTGACACCGGAACTTC 163 47 831 Antisense
ACAATCTGTTGTTGACGGACTCGCC 62 115 871 Antisense
ACAAGAGAATGGGATTCGCTCCATC 523 113 1146 Antisense
Sequence Source Affymetrix Proprietary Database