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[173985_s_at] aminotransferase

Description CE04F11 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C44E4.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=M75838 /WB_GENE_ID=C44E4.3 /WP=CE23596 /CHR=1 /FEA=Genomic Cluster /DEF=aminotransferase; CEL04F11 Chris Martin sorted cDNA library Caenorhabditis elegans cDNA clone cm04f11 5prime similar to mitochondrial aspartate aminotransferase homologous peptide, mRNA sequence.
Cluster 6grp_highest_10_10 > 79      6grp_high1_10_10 > 98      4grp_highest_8_8 > 21      4grp_high_8_8 > 23      6grp_highest_nonorm > 94      6grp_total > 96      4grp_total > 0      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 301
1 2 3 4 range
A 128.3
(-2.7)
P
115.9
(-3)
P
268
(1.1)
P
341
(3)
P
225
B 138.6
(-3)
P
164.2
(-2.4)
P
252.8
(-0.5)
P
416.4
(3)
P
278
C 153.5
(-3)
P
162.9
(-2.8)
P
338.8
(1.5)
P
401.2
(3)
P
248
avr 140.1
(-3)
147.7
(-2.8)
286.5
(0.6)
386.2
(3)
246

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1055
1 2 3 4 5 6 range
A 92
(-3)
P
148.6
(-2.6)
P
283.2
(-1.6)
P
526.9
(0.1)
P
764.2
(1.9)
P
922.2
(3)
P
830
B 105.7
(-3)
P
141.9
(-2.8)
P
207
(-2.4)
P
443.6
(-1.1)
P
798.3
(1)
P
1146.6
(3)
P
1041
C 109
(-3)
P
195.2
(-2.4)
P
216.5
(-2.2)
P
302.3
(-1.6)
P
815.4
(2.1)
P
935.2
(3)
P
826
avr 102.2
(-3)
161.9
(-2.6)
235.6
(-2.1)
424.3
(-0.9)
792.6
(1.6)
1001.3
(3)
899
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 46.7
(-0.1)
P
25.2
(-3)
P
70.1
(3)
P
49.9
(0.3)
P
45
B 44.2
(-2.5)
P
40.5
(-3)
P
58.6
(-0.7)
P
88.7
(3)
P
48
C 50.8
(-2.7)
P
46.8
(-3)
P
118.1
(3)
P
67.7
(-1.2)
P
71
avr 47.2
(-1.7)
37.5
(-3)
82.3
(3)
68.8
(1.2)
45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 411
1 2 3 4 5 6 range
A 17
(-3)
P
52.8
(-2)
P
61.7
(-1.7)
P
133.6
(0.3)
P
230.6
(3)
P
224.5
(2.8)
P
214
B 36.8
(-3)
P
47.2
(-2.8)
P
50.3
(-2.8)
P
117.5
(-1.8)
P
324.3
(1.4)
P
427.5
(3)
P
391
C 40.4
(-3)
P
59.6
(-2.6)
P
55.1
(-2.7)
P
60.8
(-2.6)
P
263.9
(1.9)
P
313.1
(3)
P
273
avr 31.4
(-3)
53.2
(-2.5)
55.7
(-2.5)
104
(-1.5)
272.9
(2)
321.7
(3)
290
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173985_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22865
Sequence Type Exemplar sequence
Representative Public ID CE04F11 NCBI
Target Description CE04F11 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C44E4.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=M75838 /WB_GENE_ID=C44E4.3 /WP=CE23596 /CHR=1 /FEA=Genomic Cluster /DEF=aminotransferase; CEL04F11 Chris Martin sorted cDNA library Caenorhabditis elegans cDNA clone cm04f11 5prime similar to mitochondrial aspartate aminotransferase homologous peptide, mRNA sequence.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059012(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_059012 NCBI Caenorhabditis elegans C44E4.3 (aminotransferase) mRNA, complete cds. 11/11 None
C44E4.3 ENSEMBL cdna:known chromosome:CEL140:I:4637504:4640883:-1 gene:C44E4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4637514-4640874(-) 100.0 100.0
Public Domain and Genome References
Gene Title aminotransferase
Ensembl C44E4.3
Entrez Gene 172072 Entrez gene
SwissProt O01804 EMBL-EBI
WormBase CE34271 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:245951_AT aspartate aminotransferase, cytoplasmic isozyme 1 / transaminase A (ASP2) at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6520 amino acid metabolism inferred from electronic annotation QuickGO AmiGO
9058 biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8483 transaminase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAD47121 Hypothetical protein C44E4.3 [Caenorhabditis elegans] ref|NP_491413.2| C44E4.3 [Caenorhabditis elegans] 0.0
blast CAE74487 Hypothetical protein CBG22238 [Caenorhabditis briggsae] 0.0
blast CAE61217 Hypothetical protein CBG05011 [Caenorhabditis briggsae] 1.0E-160
Protein Families
Method ID Description E-Value
ec AATM_HUMAN (P00505) Aspartate aminotransferase, mitochondrial precursor (EC 2.6.1.1) (Transaminase A) (Glutamate oxaloacetate transaminase-2) 0.0
ec 1A11_CUCMA (P23599) 1-aminocyclopropane-1-carboxylate synthase CMW33 (EC 4.4.1.14) (ACC synthase) (S-adenosyl-L-methionine methylthioadenosine-lyase) 0.0070
Protein Domains
Database ID Description E-Value
Pfam IPR004839 EMBL-EBI Aminotransferase, class I and II 1.6E-92
Sequence
Target Sequence
>C. ELEGANS:173985_S_AT
gttgacgatgacgcatttgctcttcgtcatttcatagaacaaggacacaatgttctcgtt
gctcaatcatttgcaaaaaatatgggattgtacggagaacgtgttggtgcattttcgatt
gtttgtgattctgctgaagaagcgattcgtgttggatctcaaatgagaattatcattcgt
ccaatgatctcaatgccaccacttcatggagcaagaattgcttcacggattttgagtaat
ccagagttgaagaaatcttggctggaagatgtgaaactaatggctgaccgtatcaaatca
atgcgtacggctctaaaagacggcctgaaagctgaaggatctactctcaactgggatcat
attaccaatcaaattggaatgttttgtttcactggaatcaatgagaaacaagttcaaaag
ctcatcaaagagcactcagtgtacttgacaaatgatggacgtatttctatttctggtatt
aatactggaaatgttgcatatttggctaaagctctccatgacgtc


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
GTTGACGATGACGCATTTGCTCTTC 218 439 830 Antisense
TGCTCTTCGTCATTTCATAGAACAA 320 581 847 Antisense
ACAAGGACACAATGTTCTCGTTGCT 690 113 868 Antisense
GAACGTGTTGGTGCATTTTCGATTG 697 347 926 Antisense
TTTTCGATTGTTTGTGATTCTGCTG 452 677 941 Antisense
GAAGCGATTCGTGTTGGATCTCAAA 201 343 968 Antisense
ATCTCAATGCCACCACTTCATGGAG 179 31 1016 Antisense
GCGTACGGCTCTAAAAGACGGCCTG 622 291 1132 Antisense
GCTGAAGGATCTACTCTCAACTGGG 316 297 1160 Antisense
GATGGACGTATTTCTATTTCTGGTA 84 407 1283 Antisense
TTTGGCTAAAGCTCTCCATGACGTC 346 613 1330 Antisense
Sequence Source Affymetrix Proprietary Database