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[173972_s_at]

Description CE3372 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C15F1.6 /5_PRIME_EXT_DB=WormBase Gene ID /GB=T02337 /WB_GENE_ID=C15F1.6 /WP=CE20509 /CHR=2 /FEA=Genomic Cluster /DEF=wEST03058 Early embryo, Stratagene (cat. no. 937007) Caenorhabditis elegans cDNA clone CESAC38, mRNA sequence.
Cluster 6grp_highest_10_10 > 14      6grp_high1_10_10 > 31      4grp_highest_8_8 > 16      4grp_high_8_8 > 60      6grp_highest_nonorm > 26      6grp_total > 42      4grp_total > 59      6grp_cgc > 81      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5729
1 2 3 4 range
A 5634.8
(1.4)
P 		7128.5
(3)
P 		7002.1
(2.9)
P 		1617.9
(-3)
P 		5511
B 6080.5
(1.8)
P 		6561.8
(2.3)
P 		7231.8
(3)
P 		1502.8
(-3)
P 		5729
C 5225.7
(1.5)
P 		6428.1
(3)
P 		6451.6
(3)
P 		1652.9
(-3)
P 		4799
avr 5647
(1.6) 		6706.1
(2.8) 		6895.2
(3) 		1591.2
(-3) 		5304

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6291
1 2 3 4 5 6 range
A 6591.6
(1.4)
P 		6387.7
(1.1)
P 		7890
(3)
P 		5400.8
(-0.1)
P 		4247.4
(-1.5)
P 		3072.8
(-3)
P 		4817
B 5505.4
(-0.6)
P 		5602.4
(-0.5)
P 		9239.9
(3)
P 		5824.6
(-0.3)
P 		3971.8
(-2)
P 		2949
(-3)
P 		6291
C 5006
(-0.6)
P 		5844.2
(0.3)
P 		8140.9
(3)
P 		5126.6
(-0.5)
P 		3948.4
(-1.9)
P 		2984.2
(-3)
P 		5157
avr 5701
(-0) 		5944.8
(0.3) 		8423.6
(3) 		5450.7
(-0.3) 		4055.9
(-1.8) 		3002
(-3) 		5422
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2012
1 2 3 4 range
A 2051.6
(3)
P 		1547.4
(1.3)
P 		1830.5
(2.3)
P 		236.8
(-3)
P 		1815
B 1939
(3)
P 		1620.6
(1.8)
P 		1675.8
(2)
P 		320.2
(-3)
P 		1619
C 1729.8
(1.4)
P 		1848.1
(1.8)
P 		2248.6
(3)
P 		278.7
(-3)
P 		1970
avr 1906.8
(3) 		1672
(2.1) 		1918.3
(3) 		278.6
(-3) 		1640

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1524
1 2 3 4 5 6 range
A 1215.1
(-1.2)
P 		2271.8
(3)
P 		1718.3
(0.8)
P 		1369.8
(-0.6)
P 		1281.7
(-0.9)
P 		748.1
(-3)
P 		1524
B 1915.6
(1.3)
P 		1864.8
(1)
P 		2243.7
(3)
P 		1542.7
(-0.7)
P 		1613.7
(-0.3)
P 		1099.7
(-3)
P 		1144
C 1855.5
(1.8)
P 		1783.3
(1.4)
P 		2072
(3)
P 		1031
(-2.8)
P 		1277.6
(-1.4)
P 		999
(-3)
P 		1073
avr 1662.1
(1) 		1973.3
(2.8) 		2011.3
(3) 		1314.5
(-0.9) 		1391
(-0.5) 		948.9
(-3) 		1062
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173972_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23736
Sequence Type Exemplar sequence
Representative Public ID CE3372 NCBI
Target Description CE3372 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C15F1.6 /5_PRIME_EXT_DB=WormBase Gene ID /GB=T02337 /WB_GENE_ID=C15F1.6 /WP=CE20509 /CHR=2 /FEA=Genomic Cluster /DEF=wEST03058 Early embryo, Stratagene (cat. no. 937007) Caenorhabditis elegans cDNA clone CESAC38, mRNA sequence.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063029(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063029 NCBI Caenorhabditis elegans steroid Alpha ReducTase family member (art-1) (art-1) mRNA, complete cds. 11/11 None
SNAP00000007584 ENSEMBL cdna:SNAP chromosome:CEL140:II:6970807:6972165:-1 10/11 None
GENEFINDER00000007596 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6970807:6972165:-1 11/11 None
C15F1.6 ENSEMBL cdna:known chromosome:CEL140:II:6970599:6972166:-1 gene:C15F1.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6970803-6972162(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol art-1
Ensembl C15F1.6
Entrez Gene 174140 Entrez gene
SwissProt Q9N5Y2 EMBL-EBI
WormBase CE20509 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251796_AT 3-oxo-5-alpha-steroid 4-dehydrogenase family protein / steroid 5-alpha-reductase family protein at
CANINE:1586406_AT similar to Synaptic glycoprotein SC2 cfa
CANINE_2:CFA.1397.1.S1_S_AT similar to Synaptic glycoprotein SC2 cfa
DROSGENOME1:141758_AT dm
DROSOPHILA_2:1634542_AT dm
CHICKEN:GGA.1440.1.S1_AT similar to synaptic glycoprotein SC2 gga
HU35KSUBA:HUM_ALU_AT glycoprotein, synaptic 2 hs
HU35KSUBB:HUM_ALU_AT glycoprotein, synaptic 2 hs
HU35KSUBC:HUM_ALU_AT glycoprotein, synaptic 2 hs
HU35KSUBD:HUM_ALU_AT glycoprotein, synaptic 2 hs
HUGENEFL:HUM_ALU_AT glycoprotein, synaptic 2 hs
HC-G110:HUM_ALU_AT glycoprotein, synaptic 2 hs
HU35KSUBA:C00180_F_AT glycoprotein, synaptic 2 hs
HU35KSUBD:RC_AA412495_AT glycoprotein, synaptic 2 hs
HU35KSUBD:RC_AA599303_AT glycoprotein, synaptic 2 hs
HG-U133A:208336_S_AT glycoprotein, synaptic 2 hs
HG-U133_PLUS_2:208336_S_AT glycoprotein, synaptic 2 hs
HG-FOCUS:208336_S_AT glycoprotein, synaptic 2 hs
HG-U133A_2:208336_S_AT glycoprotein, synaptic 2 hs
U133_X3P:G4759061_3P_S_AT glycoprotein, synaptic 2 hs
U133_X3P:HS2.98153.1.S1_3P_AT glycoprotein, synaptic 2 hs
HG-U133_PLUS_2:1569392_AT glycoprotein, synaptic 2 hs
HG-U95AV2:38966_AT glycoprotein, synaptic 2 hs
HG-U133_PLUS_2:231556_AT Glycoprotein, synaptic 2 hs
HG-U133B:231556_AT Glycoprotein, synaptic 2 hs
HG-U95C:54509_AT Glycoprotein, synaptic 2 hs
U133_X3P:HS.98153.0.A1_3P_AT Glycoprotein, synaptic 2 hs
MU11KSUBA:AA240099_S_AT glycoprotein, synaptic 2 mm
MOE430A:1429681_A_AT glycoprotein, synaptic 2 mm
MOUSE430A_2:1429681_A_AT glycoprotein, synaptic 2 mm
MOUSE430_2:1429681_A_AT glycoprotein, synaptic 2 mm
MOE430A:1416352_S_AT glycoprotein, synaptic 2 mm
MOUSE430A_2:1416352_S_AT glycoprotein, synaptic 2 mm
MOUSE430_2:1416352_S_AT glycoprotein, synaptic 2 mm
MU19KSUBA:TC18509_S_AT ES cells cDNA, RIKEN full-length enriched library, clone:2410016D23 product:SC2 homolog [Rattus sp], full insert sequence mm
MU19KSUBB:TC33270_I_AT ES cells cDNA, RIKEN full-length enriched library, clone:2410016D23 product:SC2 homolog [Rattus sp], full insert sequence mm
MU19KSUBB:TC33270_S_AT ES cells cDNA, RIKEN full-length enriched library, clone:2410016D23 product:SC2 homolog [Rattus sp], full insert sequence mm
RG-U34A:S45663_AT glycoprotein, synaptic 2 rn
RG-U34A:S45663_G_AT glycoprotein, synaptic 2 rn
RAE230A:1371358_AT glycoprotein, synaptic 2 rn
RAT230_2:1371358_AT glycoprotein, synaptic 2 rn
YEAST_2:1775760_AT Enoyl reductase that catalyzes the last step in each cycle of very long chain fatty acid elongation, localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions, coimmunoprecipitates with elongases Fen1p and Sur4p Sc
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF39753 Steroid alpha reductase family protein 1 [Caenorhabditis elegans] ref|NP_495430.1| steroid Alpha ReducTase family member (art-1) [Caenorhabditis elegans] 1.0E-169
blast AAF39753 Steroid alpha reductase family protein 1 [Caenorhabditis elegans] ref|NP_495430.1| steroid Alpha ReducTase family member (art-1) [Caenorhabditis elegans] 1.0E-167
blast CAE66005 Hypothetical protein CBG11196 [Caenorhabditis briggsae] 1.0E-161
blast CAE66005 Hypothetical protein CBG11196 [Caenorhabditis briggsae] 1.0E-158
blast XP_781465 PREDICTED: similar to Synaptic glycoprotein SC2 [Strongylocentrotus purpuratus] 4.0E-92
Protein Domains
Database ID Description E-Value
Pfam IPR000626 EMBL-EBI Ubiquitin 0.012
Pfam IPR001104 EMBL-EBI 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal 2.1E-39
Pfam IPR001104 EMBL-EBI 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal 2.1E-39
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495430.1 4 112-134,165-184,194-216,254-276
C15F1.6 4 112-134,165-184,194-216,254-276
SNAP00000007584 4 116-138,159-177,192-214,252-274
GENEFINDER00000007596 4 112-134,165-184,194-216,254-276
Sequence
Target Sequence 
>C. ELEGANS:173972_S_AT
tttgttcgtttatccgcttttctatcttcgaccaactttcatttatggacaagccgctgt
caatgctaccatgcatccagcagtacaaatcgccttctttgcatggagcttccactacgc
caagagattgttcgaaactcaattcatccatagattcggaaactcgactatgccacagtt
caatttagtcaagaactgctcctattactggggattcgctgccttcgtcgcctactttgt
caatcacccacttttcacaccaccagctttcggagatcttcaggtttatttcggacttgc
cggtttcgtgatttcggagtttggaaatctctcgatccacattcttctccgcaacctccg
tccagctggaacccgcgaacgtcgtattccaaagcctgatggaaatccactttctctcct
cttcaactacgtttcgtgcccgaactacacatatgaggtcgcatcatggatcttcttctc
cattatggtccaatctcttccagcaatcatcttcaccaccgctggattcgctcaaatggc
aatctgggcccaaggaaagcaccgcaactacctcaaggagttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGTTCGTTTATCCGCTTTTCTAT 543 667 310 Antisense
TTTTCTATCTTCGACCAACTTTCAT 28 677 327 Antisense
TGGACAAGCCGCTGTCAATGCTACC 518 545 355 Antisense
ATCCAGCAGTACAAATCGCCTTCTT 630 39 384 Antisense
GAACTGCTCCTATTACTGGGGATTC 390 349 502 Antisense
GGACTTGCCGGTTTCGTGATTTCGG 230 525 602 Antisense
TGGAACCCGCGAACGTCGTATTCCA 428 547 676 Antisense
TGAGGTCGCATCATGGATCTTCTTC 469 567 763 Antisense
ATTATGGTCCAATCTCTTCCAGCAA 312 13 791 Antisense
ATCTGGGCCCAAGGAAAGCACCGCA 124 33 851 Antisense
GCACCGCAACTACCTCAAGGAGTTC 256 315 868 Antisense
Sequence Source Affymetrix Proprietary Database