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[173966_at]

Description AV195130 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=Y74C10AL.A /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV195130 /WB_GENE_ID=Y74C10AL.A /WP=CE21753 /CHR=1 /FEA=Genomic Cluster /DEF=(ST.LOUIS) protein_id:AAF60878.1; Caenorhabditis elegans cDNA clone:yk638b1 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 95      4grp_highest_8_8 > 55      4grp_high_8_8 > 19      6grp_highest_nonorm > 78      6grp_total > 85      4grp_total > 21      6grp_highest_sub > In      6grp_high1_sub > In      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 943
1 2 3 4 range
A 1712.9
(3)
P 		1485.9
(-1.6)
P 		1515.3
(-1)
P 		1415.7
(-3)
P 		297
B 1736.4
(0.5)
P 		1697.6
(0.2)
P 		1196.8
(-3)
P 		2133.4
(3)
P 		937
C 1814.3
(0.8)
P 		1598.5
(-0.6)
P 		1234.1
(-3)
P 		2139.4
(3)
P 		905
avr 1754.5
(1.5) 		1594
(-0.1) 		1315.4
(-3) 		1896.2
(3) 		581

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1252
1 2 3 4 5 6 range
A 1803.2
(-0.2)
P 		1321.5
(-3)
P 		1462.8
(-2.2)
P 		1637.3
(-1.2)
P 		2367.7
(3)
P 		1907.2
(0.4)
P 		1046
B 1702.6
(-1.2)
P 		1414.3
(-3)
P 		1555
(-2.1)
P 		2248.3
(2.3)
P 		1600.7
(-1.8)
P 		2367.2
(3)
P 		953
C 1341.2
(-1.8)
P 		1115.7
(-3)
P 		1621.5
(-0.4)
P 		1919.6
(1.1)
P 		1710.5
(0.1)
P 		2283.6
(3)
P 		1168
avr 1615.7
(-0.8) 		1283.8
(-3) 		1546.4
(-1.3) 		1935.1
(1.3) 		1893
(1.1) 		2186
(3) 		902
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 417
1 2 3 4 range
A 623.7
(3)
P 		322.5
(-1.3)
P 		396.1
(-0.3)
P 		207.2
(-3)
P 		417
B 553.7
(3)
P 		419.3
(0.1)
P 		277.3
(-3)
P 		454.6
(0.8)
P 		276
C 600.6
(3)
P 		459.6
(-0.5)
P 		430.1
(-1.3)
P 		360.7
(-3)
P 		240
avr 592.7
(3) 		400.5
(-1.6) 		367.8
(-2.4) 		340.8
(-3) 		252

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 564
1 2 3 4 5 6 range
A 332.4
(-2.8)
P 		470
(-0.7)
P 		318.6
(-3)
P 		415.3
(-1.5)
P 		714.5
(3)
P 		464.4
(-0.8)
P 		396
B 592.4
(-0.4)
P 		470.8
(-1.9)
P 		377.6
(-3)
P 		595.5
(-0.4)
P 		650.4
(0.2)
P 		882.7
(3)
P 		505
C 497.1
(-0.8)
P 		340.4
(-3)
P 		412.7
(-2)
P 		386
(-2.4)
P 		553.5
(0)
P 		764.5
(3)
P 		424
avr 474
(-1.1) 		427.1
(-2) 		369.6
(-3) 		465.6
(-1.3) 		639.5
(1.8) 		703.9
(3) 		334
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173966_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22571
Sequence Type Exemplar sequence
Representative Public ID AV195130 NCBI
Target Description AV195130 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=Y74C10AL.A /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV195130 /WB_GENE_ID=Y74C10AL.A /WP=CE21753 /CHR=1 /FEA=Genomic Cluster /DEF=(ST.LOUIS) protein_id:AAF60878.1; Caenorhabditis elegans cDNA clone:yk638b1 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058584(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058584 NCBI Caenorhabditis elegans Y74C10AL.2 (Y74C10AL.2) mRNA, complete cds. 11/11 None
Y74C10AL.2 ENSEMBL cdna:known chromosome:CEL140:I:2468789:2471936:-1 gene:Y74C10AL.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2468810-2471929(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y74C10AL.2
Entrez Gene 171806 Entrez gene
SwissProt Q9N2X7 EMBL-EBI
WormBase CE21753 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.19281.1.S1_AT similar to Transmembrane protein 50B (HCV p7-transregulated protein 3) cfa
CANINE_2:CFAAFFX.14419.1.S1_S_AT similar to Transmembrane protein 50B (HCV p7-transregulated protein 3) cfa
CHICKEN:GGA.14006.1.A1_AT similar to Membrane protein C21orf4 (UNQ167/PRO193) gga
CHICKEN:GGAAFFX.24819.1.S1_AT similar to Membrane protein C21orf4 (UNQ167/PRO193) gga
HG-U133B:222907_X_AT transmembrane protein 50B hs
HG-U133_PLUS_2:222907_X_AT transmembrane protein 50B hs
HG-U133B:228001_AT transmembrane protein 50B hs
HG-U133_PLUS_2:228001_AT transmembrane protein 50B hs
HG-U133_PLUS_2:219600_S_AT transmembrane protein 50B hs
HG-U133A:219600_S_AT transmembrane protein 50B hs
HG-FOCUS:219600_S_AT transmembrane protein 50B hs
HG-U133A_2:219600_S_AT transmembrane protein 50B hs
HU35KSUBA:RC_AA243692_AT transmembrane protein 50B hs
HU35KSUBA:HUM_ALU_AT transmembrane protein 50B hs
HU35KSUBB:HUM_ALU_AT transmembrane protein 50B hs
HU35KSUBC:HUM_ALU_AT transmembrane protein 50B hs
HU35KSUBD:HUM_ALU_AT transmembrane protein 50B hs
HUGENEFL:HUM_ALU_AT transmembrane protein 50B hs
HC-G110:HUM_ALU_AT transmembrane protein 50B hs
HU35KSUBC:RC_W69669_AT transmembrane protein 50B hs
U133_X3P:G8659558_3P_A_AT transmembrane protein 50B hs
U133_X3P:HS.9042.2.A1_3P_AT transmembrane protein 50B hs
U133_X3P:HS.9042.0.A1_3P_AT transmembrane protein 50B hs
HG-U133_PLUS_2:225182_AT transmembrane protein 50B hs
HG-U133B:225182_AT transmembrane protein 50B hs
HG-U95C:52862_R_AT transmembrane protein 50B hs
HG-U95B:57721_AT transmembrane protein 50B hs
HG-U95C:60894_AT transmembrane protein 50B hs
HG-U95D:75102_AT transmembrane protein 50B hs
HG-U133_PLUS_2:231696_X_AT Transmembrane protein 50B hs
HG-U133B:231696_X_AT Transmembrane protein 50B hs
HU35KSUBD:RC_D45596_AT Transmembrane protein 50B hs
MU11KSUBA:AA684097_G_AT transmembrane protein 50B mm
MU11KSUBA:AA684097_AT transmembrane protein 50B mm
MG-U74AV2:96340_AT transmembrane protein 50B mm
MG-U74AV2:161222_R_AT transmembrane protein 50B mm
MOE430A:1423707_AT transmembrane protein 50B mm
MOUSE430A_2:1423707_AT transmembrane protein 50B mm
MOUSE430_2:1423707_AT transmembrane protein 50B mm
MU19KSUBC:TC38856_AT Transmembrane protein 50B (Tmem50b), mRNA mm
RG-U34B:RC_AI060197_AT similar to RIKEN cDNA B230114J08 (predicted) rn
RG-U34B:RC_AI044030_F_AT similar to RIKEN cDNA B230114J08 (predicted) rn
RAE230A:1371347_AT similar to RIKEN cDNA B230114J08 (predicted) rn
RAT230_2:1371347_AT similar to RIKEN cDNA B230114J08 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAF60878 Hypothetical protein Y74C10AL.2 [Caenorhabditis elegans] ref|NP_490985.1| Y74C10AL.2 [Caenorhabditis elegans] 3.0E-75
blast CAE60387 Hypothetical protein CBG03988 [Caenorhabditis briggsae] 7.0E-68
blast AAH97652 Unknown (protein for MGC:114915) [Xenopus laevis] 9.0E-31
Protein Domains
Database ID Description E-Value
Pfam IPR007919 EMBL-EBI Protein of unknown function UPF0220 1.3E-31
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_490985.1 4 21-40,50-72,93-115,125-147
Y74C10AL.2 4 21-40,50-72,93-115,125-147
Sequence
Target Sequence 
>C. ELEGANS:173966_AT
gagatgtggggataagcgcggatgctcctggattcggattttattctcgaaaaaaaattc
aaaaattttctcttttttttcatattccactacttattcgtcattttccttgtccatttc
ccttattacagtaccccat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGATGTGGGGATAAGCGCGGATGC 597 393 473 Antisense
TGTGGGGATAAGCGCGGATGCTCCT 239 553 477 Antisense
CGGATGCTCCTGGATTCGGATTTTA 485 243 491 Antisense
TTTTTTTCATATTCCACTACTTATT 251 675 546 Antisense
TTCATATTCCACTACTTATTCGTCA 318 689 551 Antisense
ATTCCACTACTTATTCGTCATTTTC 604 5 556 Antisense
CCACTACTTATTCGTCATTTTCCTT 565 273 559 Antisense
TACTTATTCGTCATTTTCCTTGTCC 603 643 563 Antisense
CTTGTCCATTTCCCTTATTACAGTA 602 217 581 Antisense
TGTCCATTTCCCTTATTACAGTACC 686 555 583 Antisense
CATTTCCCTTATTACAGTACCCCAT 144 215 587 Antisense
Sequence Source Affymetrix Proprietary Database