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[173954_at]

Description CEK009H3R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F31B12.1A /5_PRIME_EXT_DB=WormBase Gene ID /GB=D28105 /WB_GENE_ID=F31B12.1A /WP=CE26713 /CHR=X /FEA=Genomic Cluster /GEN=plc-1 /DEF=phosphoinositide-specific phospholipase C; C.elegans cDNA clone yk9h3 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 29      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 70      6grp_total > 27      4grp_total > 14      6grp_highest_sub > Cu      6grp_cgc > 04      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 500
1 2 3 4 range
A 412.3
(0.1)
P 		154.7
(-3)
P 		163.1
(-2.9)
P 		655.1
(3)
P 		500
B 419.4
(2.6)
P 		235.2
(-1.9)
P 		189.1
(-3)
P 		434.3
(3)
P 		245
C 486.8
(3)
P 		215.8
(-2.4)
P 		186.4
(-3)
P 		441.1
(2.1)
P 		300
avr 439.5
(1.7) 		201.9
(-2.6) 		179.5
(-3) 		510.2
(3) 		331

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 216
1 2 3 4 5 6 range
A 383.3
(2.6)
P 		394.6
(3)
P 		217.2
(-2.7)
P 		208.8
(-3)
P 		295.2
(-0.2)
P 		260.8
(-1.3)
P 		186
B 357.5
(3)
P 		327.2
(1.6)
P 		230.8
(-3)
P 		237.1
(-2.7)
P 		289.6
(-0.2)
P 		291.4
(-0.1)
P 		127
C 381.9
(3)
P 		366.9
(2.6)
P 		229.5
(-1.5)
P 		178.5
(-3)
P 		252.5
(-0.8)
P 		270.4
(-0.3)
P 		203
avr 374.2
(3) 		362.9
(2.6) 		225.8
(-2.4) 		208.1
(-3) 		279.1
(-0.4) 		274.2
(-0.6) 		166
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 128
1 2 3 4 range
A 150.1
(3)
P 		33.6
(-3)
P 		42.6
(-2.5)
P 		95.9
(0.2)
P 		117
B 133.7
(3)
P 		58.1
(-2)
P 		43.8
(-3)
P 		92.5
(0.3)
P 		90
C 161.2
(3)
P 		62
(-3)
P 		65
(-2.8)
P 		74.4
(-2.3)
P 		99
avr 148.3
(3) 		51.2
(-3) 		50.5
(-3) 		87.6
(-0.7) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 106
1 2 3 4 5 6 range
A 70.7
(-1.5)
P 		140.4
(3)
P 		47.3
(-3)
P 		53
(-2.6)
P 		89.1
(-0.3)
P 		63.5
(-2)
P 		93
B 124.4
(3)
P 		108.9
(1.6)
P 		56
(-3)
P 		62.8
(-2.4)
P 		117.7
(2.4)
P 		108.6
(1.6)
P 		68
C 141.6
(3)
P 		111.9
(1.3)
P 		58.4
(-1.7)
P 		35.9
(-3)
P 		81.7
(-0.4)
P 		90.5
(0.1)
P 		106
avr 112.2
(2.3) 		120.4
(3) 		53.9
(-2.7) 		50.6
(-3) 		96.2
(0.9) 		87.5
(0.2) 		70
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173954_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25949
Sequence Type Exemplar sequence
Representative Public ID CEK009H3R NCBI
Target Description CEK009H3R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F31B12.1A /5_PRIME_EXT_DB=WormBase Gene ID /GB=D28105 /WB_GENE_ID=F31B12.1A /WP=CE26713 /CHR=X /FEA=Genomic Cluster /GEN=plc-1 /DEF=phosphoinositide-specific phospholipase C; C.elegans cDNA clone yk9h3 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077404(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077404 NCBI Caenorhabditis elegans PhosphoLipase C family member (plc-1) (plc-1) mRNA, complete cds. 11/11 None
F31B12.1b ENSEMBL cdna:known chromosome:CEL140:X:10796082:10812457:-1 gene:F31B12.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
181274 3 1 NM_001029446 175735_at (11)
NM_077404 173954_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:10796097-10812458(-) 100.0 100.0
Public Domain and Genome References
Ensembl F31B12.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB60299 Hypothetical protein F31B12.1b [Caenorhabditis elegans] emb|CAB60282.4| Hypothetical protein F31B12.1b [Caenorhabditis elegans] ref|NP_509805.3| PhosphoLipase C family member (plc-1) [Caenorhabditis elegans] 0.0
blast CAD44153 Hypothetical protein F31B12.1c [Caenorhabditis elegans] emb|CAD44124.1| Hypothetical protein F31B12.1c [Caenorhabditis elegans] ref|NP_001024619.1| PhosphoLipase C family member (plc-1) [Caenorhabditis elegans] 0.0
blast CAB60298 Hypothetical protein F31B12.1a [Caenorhabditis elegans] emb|CAB60281.3| Hypothetical protein F31B12.1a [Caenorhabditis elegans] ref|NP_001024617.1| PhosphoLipase C family member (plc-1) [Caenorhabditis elegans] gb|AAC38963.1| phospholipase C PLC210 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000159 EMBL-EBI RA domain 1.0E-19
Pfam IPR000159 EMBL-EBI RA domain 6.5E-22
Pfam IPR000909 EMBL-EBI Phosphatidylinositol-specific phospholipase C, X domain 2.5E-68
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 9.0E-35
Pfam IPR000008 EMBL-EBI C2 domain 2.0E-14
Sequence
Target Sequence 
>C. ELEGANS:173954_AT
tgatgatgcacatatactttccctccgatcaactcggtttcactcaattatcactatcaa
tacttctattcctatcactttcctcaaaattcactttaaacacacttttccatttctatc
cgtcttattgaattattatgttcgatcaagaattctgcatgtttgtttgcataatcgtgt
gtattattgatatactatgtaaatccctatttttcatacaagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGATGATGCACATATACTTTCCCTC 358 567 5753 Antisense
CCTCCGATCAACTCGGTTTCACTCA 436 267 5774 Antisense
GGTTTCACTCAATTATCACTATCAA 71 507 5788 Antisense
CAATACTTCTATTCCTATCACTTTC 465 181 5810 Antisense
ATCACTTTCCTCAAAATTCACTTTA 123 29 5826 Antisense
ATTCACTTTAAACACACTTTTCCAT 564 9 5841 Antisense
TAAACACACTTTTCCATTTCTATCC 220 641 5849 Antisense
TTTCCATTTCTATCCGTCTTATTGA 336 669 5859 Antisense
GATCAAGAATTCTGCATGTTTGTTT 406 421 5896 Antisense
GTTTGTTTGCATAATCGTGTGTATT 97 453 5913 Antisense
TAAATCCCTATTTTTCATACAAGAA 597 639 5952 Antisense
Sequence Source Affymetrix Proprietary Database