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[173937_at]

Description CE08A2 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F30F8.1 /5_PRIME_EXT_DB=WormBase Gene ID /GB=M89391 /WB_GENE_ID=F30F8.1 /WP=CE09813 /CHR=1 /FEA=Genomic Cluster /DEF=CEL08A2 Chris Martin sorted cDNA library Caenorhabditis elegans cDNA clone cm08a2 5prime, mRNA sequence.
Cluster 6grp_total > 97      4grp_total > 40      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 80.3
(-3)
A 		85.1
(-2.6)
A 		144.9
(3)
A 		104.1
(-0.8)
P 		65
B 68.8
(-3)
A 		100.5
(-1)
A 		165.2
(3)
P 		91.7
(-1.6)
P 		96
C 78.2
(-3)
A 		105.5
(1.3)
A 		115.9
(3)
P 		104.1
(1.1)
P 		38
avr 75.8
(-3) 		97
(-1.1) 		142
(3) 		100
(-0.8) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 654
1 2 3 4 5 6 range
A 42.7
(-3)
A 		91.7
(-2.5)
A 		90.3
(-2.5)
P 		146.6
(-1.9)
P 		490.9
(1.7)
P 		614.5
(3)
P 		572
B 58.6
(-3)
A 		84.9
(-2.7)
A 		74.5
(-2.8)
A 		254.7
(-0.9)
P 		408.8
(0.7)
P 		628.7
(3)
P 		570
C 54.4
(-3)
A 		96.7
(-2.6)
A 		105.1
(-2.5)
A 		225.7
(-1.4)
P 		327.8
(-0.4)
P 		696.5
(3)
P 		642
avr 51.9
(-3) 		91.1
(-2.6) 		90
(-2.6) 		209
(-1.4) 		409.2
(0.6) 		646.6
(3) 		595
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 29.2
(0.7)
A 		18.5
(-2.1)
A 		37.9
(3)
A 		15.2
(-3)
P 		23
B 22
(-2.2)
A 		24.8
(-1.3)
A 		38.3
(3)
P 		19.5
(-3)
P 		19
C 25.9
(-0.8)
A 		30.3
(0.3)
A 		40.4
(3)
P 		17.6
(-3)
P 		23
avr 25.7
(-0.7) 		24.5
(-1) 		38.9
(3) 		17.4
(-3) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 227
1 2 3 4 5 6 range
A 7.9
(-3)
A 		32.6
(-2)
A 		19.7
(-2.5)
P 		37.2
(-1.8)
P 		148.1
(2.9)
P 		149.6
(3)
P 		142
B 20.4
(-2.9)
A 		28.3
(-2.7)
A 		18.1
(-3)
A 		67.4
(-1.6)
P 		166.1
(1.1)
P 		234.4
(3)
P 		216
C 20.2
(-3)
A 		29.5
(-2.7)
A 		26.8
(-2.8)
A 		45.4
(-2.3)
P 		106.1
(-0.6)
P 		233.2
(3)
P 		213
avr 16.2
(-3) 		30.1
(-2.6) 		21.5
(-2.8) 		50
(-1.9) 		140.1
(0.9) 		205.7
(3) 		190
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173937_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22802
Sequence Type Exemplar sequence
Representative Public ID CE08A2 NCBI
Target Description CE08A2 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F30F8.1 /5_PRIME_EXT_DB=WormBase Gene ID /GB=M89391 /WB_GENE_ID=F30F8.1 /WP=CE09813 /CHR=1 /FEA=Genomic Cluster /DEF=CEL08A2 Chris Martin sorted cDNA library Caenorhabditis elegans cDNA clone cm08a2 5prime, mRNA sequence.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059762(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059762 NCBI Caenorhabditis elegans F30F8.1 (F30F8.1) mRNA, complete cds. 11/11 A 
F30F8.1 ENSEMBL cdna:known chromosome:CEL140:I:7840884:7843530:-1 gene:F30F8.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7840792-7843399(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F30F8.1
Entrez Gene 172548 Entrez gene
SwissProt Q93649 EMBL-EBI
WormBase CE09813 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03029 Hypothetical protein F30F8.1 [Caenorhabditis elegans] ref|NP_492163.1| F30F8.1 [Caenorhabditis elegans] 0.0
blast CAE56119 Hypothetical protein CBG23727 [Caenorhabditis briggsae] 1.0E-67
blast CAE56118 Hypothetical protein CBG23726 [Caenorhabditis briggsae] 1.0E-62
Sequence
Target Sequence 
>C. ELEGANS:173937_AT
gatgcttggcgttgaaggagccacaatccaatttctgaatgtctcatttccaacacttgt
aacattcactaaatatctttcatccgattcccctacatttttttcgctcccgtattcgtt
tctcgatacatctcttatattatttgttcgcttctcgacatgttccgataaccccactgt
tattgtttcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGCTTGGCGTTGAAGGAGCCACA 530 411 1937 Antisense
GAAGGAGCCACAATCCAATTTCTGA 59 335 1950 Antisense
ATCCAATTTCTGAATGTCTCATTTC 175 41 1962 Antisense
GTCTCATTTCCAACACTTGTAACAT 219 469 1977 Antisense
CTAAATATCTTTCATCCGATTCCCC 680 225 2005 Antisense
CTCCCGTATTCGTTTCTCGATACAT 358 233 2043 Antisense
TTCGTTTCTCGATACATCTCTTATA 218 695 2051 Antisense
CTCTTATATTATTTGTTCGCTTCTC 115 231 2068 Antisense
TATTTGTTCGCTTCTCGACATGTTC 316 661 2077 Antisense
TTCTCGACATGTTCCGATAACCCCA 628 691 2088 Antisense
GATAACCCCACTGTTATTGTTTCCA 118 365 2103 Antisense
Sequence Source Affymetrix Proprietary Database