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[173927_at] PH (pleckstrin homology) domain (2 domains)

Description CEK026C9R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F10G8.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D33109 /WB_GENE_ID=F10G8.8 /WP=CE23641 /CHR=1 /FEA=Genomic Cluster /DEF=PH (pleckstrin homology) domain (2 domains); C.elegans cDNA clone yk26c9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 81      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 63      6grp_total > 90      4grp_total > 47      6grp_highest_sub > 04_05      6grp_high1_sub > 04      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 302
1 2 3 4 range
A 526.5
(3)
P 		243.6
(-3)
P 		310
(-1.6)
P 		318.5
(-1.4)
P 		283
B 545.7
(3)
P 		352.6
(-2.3)
P 		326.7
(-3)
P 		333.8
(-2.8)
P 		219
C 459.7
(3)
P 		308.9
(-3)
P 		369.7
(-0.6)
P 		350.8
(-1.3)
P 		151
avr 510.6
(3) 		301.7
(-3) 		335.5
(-2) 		334.4
(-2.1) 		209

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 574
1 2 3 4 5 6 range
A 589.5
(-1.7)
P 		598.6
(-1.5)
P 		518.7
(-3)
P 		720.2
(0.8)
P 		834.2
(3)
P 		546
(-2.5)
P 		316
B 657.4
(0.4)
P 		620.6
(0)
P 		332
(-3)
P 		905.7
(3)
P 		698.9
(0.8)
P 		513.4
(-1.1)
P 		574
C 656.1
(-0.2)
P 		654.9
(-0.2)
P 		569.9
(-1.3)
P 		900.8
(3)
P 		654.3
(-0.2)
P 		436.7
(-3)
P 		464
avr 634.3
(-0.4) 		624.7
(-0.5) 		473.5
(-3) 		842.2
(3) 		729.1
(1.2) 		498.7
(-2.6) 		369
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 191.7
(3)
P 		52.9
(-2.7)
P 		81
(-1.6)
P 		46.6
(-3)
P 		145
B 174
(3)
P 		87.1
(-2.1)
P 		75.7
(-2.7)
P 		71.1
(-3)
P 		103
C 152.2
(3)
P 		88.8
(-1.1)
P 		128.9
(1.5)
P 		59.2
(-3)
P 		93
avr 172.6
(3) 		76.3
(-2.1) 		95.2
(-1.1) 		59
(-3) 		114

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 203
1 2 3 4 5 6 range
A 108.7
(-3)
P 		212.9
(1.4)
P 		113
(-2.8)
P 		182.7
(0.1)
P 		251.7
(3)
P 		132.9
(-2)
P 		143
B 228.7
(1.4)
P 		206.6
(0.7)
P 		80.6
(-3)
P 		239.9
(1.7)
P 		283.9
(3)
P 		191.5
(0.3)
P 		203
C 243.2
(3)
P 		199.8
(0.3)
P 		145
(-3)
P 		181.2
(-0.8)
P 		211.7
(1.1)
P 		146.2
(-2.9)
P 		98
avr 193.5
(0.6) 		206.4
(1.1) 		112.9
(-3) 		201.3
(0.9) 		249.1
(3) 		156.9
(-1.1) 		136
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173927_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22677
Sequence Type Exemplar sequence
Representative Public ID CEK026C9R NCBI
Target Description CEK026C9R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F10G8.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D33109 /WB_GENE_ID=F10G8.8 /WP=CE23641 /CHR=1 /FEA=Genomic Cluster /DEF=PH (pleckstrin homology) domain (2 domains); C.elegans cDNA clone yk26c9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060254(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060254 NCBI Caenorhabditis elegans F10G8.8 (F10G8.8) mRNA, complete cds. 11/11 None
F10G8.8 ENSEMBL cdna:known chromosome:CEL140:I:10058815:10070129:-1 gene:F10G8.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10058756-10070040(-) 96.59 100.0
Public Domain and Genome References
Gene Title PH (pleckstrin homology) domain (2 domains)
Ensembl F10G8.8
Entrez Gene 172870 Entrez gene
SwissProt O62399 EMBL-EBI
WormBase CE30751 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1627054_AT outspread dm
DROSGENOME1:153803_AT outspread dm
Protein Similarities
Method ID Description E-Value
blast CAB02286 Hypothetical protein F10G8.8 [Caenorhabditis elegans] emb|CAA19441.4| Hypothetical protein F10G8.8 [Caenorhabditis elegans] ref|NP_492655.2| F10G8.8 [Caenorhabditis elegans] 0.0
blast CAE60221 Hypothetical protein CBG03790 [Caenorhabditis briggsae] 0.0
blast AAH73109 LOC443626 protein [Xenopus laevis] 5.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.2E-8
Pfam IPR001849 EMBL-EBI Pleckstrin-like 7.6E-14
Sequence
Target Sequence 
>C. ELEGANS:173927_AT
tttaagttttattcgatcaaatcgtgtttcttccccatcctttgttgtagtcaattacta
atatttgaattcgctctcactccggctaaccataatttatctgtgatattcgtgtatttt
cttcttttcttataatttttcaattgttctacttgtttctagaaaatgtttgtatgtctg
gtgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTAAGTTTTATTCGATCAAATCGT 643 671 3146 Antisense
TTCGATCAAATCGTGTTTCTTCCCC 680 695 3157 Antisense
TTCCCCATCCTTTGTTGTAGTCAAT 282 697 3176 Antisense
CATCCTTTGTTGTAGTCAATTACTA 46 211 3181 Antisense
ACTAATATTTGAATTCGCTCTCACT 327 101 3202 Antisense
CTCTCACTCCGGCTAACCATAATTT 460 229 3219 Antisense
ACTCCGGCTAACCATAATTTATCTG 52 99 3224 Antisense
GGCTAACCATAATTTATCTGTGATA 335 539 3229 Antisense
GTGATATTCGTGTATTTTCTTCTTT 160 485 3248 Antisense
TTTCAATTGTTCTACTTGTTTCTAG 539 673 3283 Antisense
TAGAAAATGTTTGTATGTCTGGTGC 471 651 3305 Antisense
Sequence Source Affymetrix Proprietary Database