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[173926_at] Propionyl-coA carboxylase alpha chain precursor

Description CEC7735 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F27D9.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C12773 /WB_GENE_ID=F27D9.5 /WP=CE04451 /CHR=X /FEA=Genomic Cluster /DEF=Propionyl-coA carboxylase alpha chain precursor; C.elegans cDNA clone yk155h10 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 22      4grp_highest_8_8 > 32      4grp_high_8_8 > 51      6grp_highest_nonorm > 15      6grp_total > 32      4grp_total > 61      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5866
1 2 3 4 range
A 6766.9
(3)
P 		5912.7
(2.1)
P 		5744.6
(1.9)
P 		930.4
(-3)
P 		5837
B 6147.8
(3)
P 		5072.8
(1.7)
P 		5683.4
(2.5)
P 		1065.4
(-3)
P 		5082
C 6655.9
(3)
P 		5661.4
(2)
P 		5213.4
(1.5)
P 		901.3
(-3)
P 		5755
avr 6523.5
(3) 		5549
(1.9) 		5547.1
(1.9) 		965.7
(-3) 		5558

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3800
1 2 3 4 5 6 range
A 4641.8
(-0.9)
P 		5798.5
(1.7)
P 		6385
(3)
P 		4692.4
(-0.8)
P 		5115.6
(0.1)
P 		3732.2
(-3)
P 		2653
B 6113.4
(3)
P 		5720.2
(1.7)
P 		5056.4
(-0.4)
P 		5707.5
(1.7)
P 		4670.1
(-1.6)
P 		4231.6
(-3)
P 		1882
C 6692.9
(3)
P 		4903.5
(0.2)
P 		6658.3
(2.9)
P 		3609.6
(-1.9)
P 		5843.3
(1.7)
P 		2892.9
(-3)
P 		3800
avr 5816
(2.5) 		5474.1
(1.6) 		6033.2
(3) 		4669.8
(-0.4) 		5209.7
(1) 		3618.9
(-3) 		2414
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2328
1 2 3 4 range
A 2463.8
(3)
P 		1283.5
(-0)
P 		1501.7
(0.5)
P 		136.2
(-3)
P 		2328
B 1960.5
(3)
P 		1252.9
(0.6)
P 		1317
(0.8)
P 		227
(-3)
P 		1734
C 2203.3
(3)
P 		1627.7
(1.3)
P 		1817
(1.9)
P 		152
(-3)
P 		2051
avr 2209.2
(3) 		1388
(0.6) 		1545.2
(1) 		171.7
(-3) 		2038

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1755
1 2 3 4 5 6 range
A 855.7
(-3)
P 		2062.2
(3)
P 		1390.5
(-0.3)
P 		1190.1
(-1.3)
P 		1543.7
(0.4)
P 		908.7
(-2.7)
P 		1207
B 2127.2
(3)
P 		1904
(1.5)
P 		1227.9
(-3)
P 		1511.7
(-1.1)
P 		1897.4
(1.5)
P 		1577.9
(-0.7)
P 		899
C 2480.8
(3)
P 		1496.2
(-0.4)
P 		1694.7
(0.3)
P 		725.9
(-3)
P 		1890.8
(1)
P 		968.4
(-2.2)
P 		1755
avr 1821.2
(3) 		1820.8
(3) 		1437.7
(-0.4) 		1142.6
(-3) 		1777.3
(2.6) 		1151.7
(-2.9) 		679
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173926_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25939
Sequence Type Exemplar sequence
Representative Public ID CEC7735 NCBI
Target Description CEC7735 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F27D9.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C12773 /WB_GENE_ID=F27D9.5 /WP=CE04451 /CHR=X /FEA=Genomic Cluster /DEF=Propionyl-coA carboxylase alpha chain precursor; C.elegans cDNA clone yk155h10 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076892(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076892 NCBI Caenorhabditis elegans F27D9.5 (F27D9.5) mRNA, complete cds. 11/11 None
F27D9.5.2 ENSEMBL cdna:known chromosome:CEL140:X:7657477:7660927:-1 gene:F27D9.5 11/11 None
F27D9.5.1 ENSEMBL cdna:known chromosome:CEL140:X:7657477:7660925:-1 gene:F27D9.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7657501-7660917(-) 100.0 100.0
Public Domain and Genome References
Gene Title Propionyl-coA carboxylase alpha chain precursor
Ensembl F27D9.5
Entrez Gene 181026 Entrez gene
SwissProt Q19842 EMBL-EBI
WormBase CE04451 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1593972_AT similar to propionyl-Coenzyme A carboxylase, alpha polypeptide precursor cfa
CANINE_2:CFA.16464.1.S1_AT similar to propionyl-Coenzyme A carboxylase, alpha polypeptide precursor cfa
CANINE_2:CFA.20956.1.S1_AT similar to propionyl-Coenzyme A carboxylase, alpha polypeptide precursor cfa
CANINE_2:CFAAFFX.9732.1.S1_AT similar to propionyl-Coenzyme A carboxylase, alpha polypeptide precursor cfa
CHICKEN:GGAAFFX.25098.1.S1_AT similar to Propionyl-Coenzyme A carboxylase, alpha polypeptide precursor gga
CHICKEN:GGAAFFX.25098.2.S1_AT similar to Propionyl-Coenzyme A carboxylase, alpha polypeptide precursor gga
CHICKEN:GGAAFFX.25098.2.S1_S_AT similar to Propionyl-Coenzyme A carboxylase, alpha polypeptide precursor gga
HG-FOCUS:203860_AT propionyl Coenzyme A carboxylase, alpha polypeptide hs
HG-U133_PLUS_2:203860_AT propionyl Coenzyme A carboxylase, alpha polypeptide hs
HG-U133A_2:203860_AT propionyl Coenzyme A carboxylase, alpha polypeptide hs
HG-U133A:203860_AT propionyl Coenzyme A carboxylase, alpha polypeptide hs
HG-U95AV2:1348_S_AT propionyl Coenzyme A carboxylase, alpha polypeptide hs
HC-G110:1348_S_AT propionyl Coenzyme A carboxylase, alpha polypeptide hs
HUGENEFL:S79219_S_AT propionyl Coenzyme A carboxylase, alpha polypeptide hs
U133_X3P:G4557832_3P_X_AT propionyl Coenzyme A carboxylase, alpha polypeptide hs
U133_X3P:G4557832_3P_AT propionyl Coenzyme A carboxylase, alpha polypeptide hs
HG-U95AV2:37593_AT propionyl Coenzyme A carboxylase, alpha polypeptide hs
HG-U133_PLUS_2:216533_AT Propionyl Coenzyme A carboxylase, alpha polypeptide hs
HG-U133A_2:216533_AT Propionyl Coenzyme A carboxylase, alpha polypeptide hs
HG-U133A:216533_AT Propionyl Coenzyme A carboxylase, alpha polypeptide hs
HG-U133_PLUS_2:232376_AT Propionyl Coenzyme A carboxylase, alpha polypeptide hs
HG-U133B:232376_AT Propionyl Coenzyme A carboxylase, alpha polypeptide hs
HU35KSUBD:RC_AA609177_AT Propionyl Coenzyme A carboxylase, alpha polypeptide hs
U133_X3P:HS.274557.0.S1_3P_AT Propionyl Coenzyme A carboxylase, alpha polypeptide hs
U133_X3P:HS.109363.0.S1_3P_AT Propionyl Coenzyme A carboxylase, alpha polypeptide hs
HG-U95B:58743_AT Propionyl Coenzyme A carboxylase, alpha polypeptide hs
HG-U95E:67296_AT Propionyl Coenzyme A carboxylase, alpha polypeptide hs
MG-U74BV2:106267_S_AT propionyl-Coenzyme A carboxylase, alpha polypeptide mm
MG-U74BV2:106268_AT propionyl-Coenzyme A carboxylase, alpha polypeptide mm
MOE430A:1451405_AT propionyl-Coenzyme A carboxylase, alpha polypeptide mm
MOUSE430A_2:1451405_AT propionyl-Coenzyme A carboxylase, alpha polypeptide mm
MOUSE430_2:1451405_AT propionyl-Coenzyme A carboxylase, alpha polypeptide mm
MOE430B:1447577_X_AT propionyl-Coenzyme A carboxylase, alpha polypeptide mm
MOUSE430_2:1447577_X_AT propionyl-Coenzyme A carboxylase, alpha polypeptide mm
MU19KSUBB:TC32680_AT Propionyl-Coenzyme A carboxylase, alpha polypeptide (Pcca), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
9374 biotin binding inferred from electronic annotation QuickGO AmiGO
16874 ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA93384 Hypothetical protein F27D9.5 [Caenorhabditis elegans] ref|NP_509293.1| F27D9.5 [Caenorhabditis elegans] 0.0
blast CAE70254 Hypothetical protein CBG16755 [Caenorhabditis briggsae] 0.0
blast NP_001002746 propionyl-Coenzyme A carboxylase, alpha polypeptide [Danio rerio] gb|AAH76408.1| Propionyl-Coenzyme A carboxylase, alpha polypeptide [Danio rerio] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005482 EMBL-EBI Biotin carboxylase, C-terminal 9.5E-62
Pfam IPR005481 EMBL-EBI Carbamoyl-phosphate synthetase large chain, N-terminal 4.0E-48
Pfam IPR005479 EMBL-EBI Carbamoyl-phosphate synthase L chain, ATP-binding 8.4E-106
Pfam IPR000089 EMBL-EBI Biotin/lipoyl attachment 1.1E-21
Sequence
Target Sequence 
>C. ELEGANS:173926_AT
gagctcgagtagattattctcactgaacctgaattttttttgttgttttaattaccgttt
ttttcctaattttgtacaattcaaagccgccactatctggtatggagattgataatttca
ttcgcagtttctcaaatttcaagccatatttatatttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCTCGAGTAGATTATTCTCACTG 645 387 2177 Antisense
GTAGATTATTCTCACTGAACCTGAA 78 457 2185 Antisense
CTCACTGAACCTGAATTTTTTTTGT 133 227 2195 Antisense
TGTACAATTCAAAGCCGCCACTATC 550 557 2249 Antisense
CAATTCAAAGCCGCCACTATCTGGT 148 181 2253 Antisense
CAAAGCCGCCACTATCTGGTATGGA 412 191 2258 Antisense
GCCGCCACTATCTGGTATGGAGATT 654 285 2262 Antisense
GATAATTTCATTCGCAGTTTCTCAA 120 423 2287 Antisense
TTCATTCGCAGTTTCTCAAATTTCA 198 689 2293 Antisense
GCAGTTTCTCAAATTTCAAGCCATA 211 313 2300 Antisense
AAATTTCAAGCCATATTTATATTTT 46 117 2310 Antisense
Sequence Source Affymetrix Proprietary Database