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[173921_at]

Description CEK008B4F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F55C12.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D26865 /WB_GENE_ID=F55C12.5 /WP=CE02774 /CHR=2 /FEA=Genomic Cluster /DEF=(ST.LOUIS) TR:Q20810 protein_id:AAC71164.1; C.elegans cDNA clone yk8b4 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 97      4grp_highest_8_8 > 46      4grp_high_8_8 > 10      6grp_highest_nonorm > 84      6grp_total > 95      4grp_total > 14      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 547
1 2 3 4 range
A 566.3
(3)
P 		239
(-3)
P 		361.2
(-0.8)
P 		497
(1.7)
P 		327
B 566.2
(0.7)
P 		422.4
(-0.8)
P 		226.4
(-3)
P 		773
(3)
P 		547
C 574.4
(2.3)
P 		343.6
(-2)
P 		287.9
(-3)
P 		614.3
(3)
P 		326
avr 569
(1.9) 		335
(-2.2) 		291.8
(-3) 		628.1
(3) 		336

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 480
1 2 3 4 5 6 range
A 608.3
(0.2)
P 		539.2
(-0.8)
P 		383.9
(-3)
P 		611.1
(0.2)
P 		807.3
(3)
P 		717.1
(1.7)
P 		423
B 510.5
(-3)
P 		600.2
(-1.5)
P 		575.6
(-1.9)
P 		743.2
(1)
P 		728.1
(0.7)
P 		863.4
(3)
P 		353
C 548.7
(-1.8)
P 		559.9
(-1.4)
P 		507.3
(-3)
P 		709.4
(3)
P 		569.4
(-1.2)
P 		680.8
(2.2)
P 		202
avr 555.8
(-1.5) 		566.4
(-1.2) 		488.9
(-3) 		687.9
(1.5) 		701.6
(1.8) 		753.8
(3) 		265
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 206.2
(3)
P 		51.9
(-3)
P 		94.4
(-1.3)
P 		72.7
(-2.2)
P 		154
B 180.6
(3)
P 		104.3
(-0.6)
P 		52.5
(-3)
P 		164.7
(2.3)
P 		128
C 190.1
(3)
P 		98.8
(-3)
P 		100.3
(-2.9)
P 		103.6
(-2.7)
P 		91
avr 192.3
(3) 		85
(-2.9) 		82.4
(-3) 		113.7
(-1.3) 		110

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 238
1 2 3 4 5 6 range
A 112.1
(-1.9)
P 		191.8
(1.1)
P 		83.6
(-3)
P 		155
(-0.3)
P 		243.6
(3)
P 		174.6
(0.4)
P 		160
B 177.6
(-1.8)
P 		199.8
(-1)
P 		139.8
(-3)
P 		196.9
(-1.1)
P 		295.8
(2.1)
P 		322
(3)
P 		182
C 203.4
(1.5)
P 		170.9
(-0.5)
P 		129.1
(-3)
P 		142.7
(-2.2)
P 		184.2
(0.3)
P 		227.9
(3)
P 		99
avr 164.4
(-0.7) 		187.5
(0.4) 		117.5
(-3) 		164.9
(-0.7) 		241.2
(3) 		241.5
(3) 		124
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173921_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23556
Sequence Type Exemplar sequence
Representative Public ID CEK008B4F NCBI
Target Description CEK008B4F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F55C12.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D26865 /WB_GENE_ID=F55C12.5 /WP=CE02774 /CHR=2 /FEA=Genomic Cluster /DEF=(ST.LOUIS) TR:Q20810 protein_id:AAC71164.1; C.elegans cDNA clone yk8b4 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062770(11), NM_182235(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062770 NCBI Caenorhabditis elegans F55C12.5b (F55C12.5) mRNA, complete cds. 11/11 None
NM_182235 NCBI Caenorhabditis elegans F55C12.5c (F55C12.5) mRNA, complete cds. 11/11 None
F55C12.5b ENSEMBL cdna:known chromosome:CEL140:II:5867219:5873527:-1 gene:F55C12.5 11/11 A 
F55C12.5c ENSEMBL cdna:known chromosome:CEL140:II:5867232:5873578:-1 gene:F55C12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173997 3 2 NM_062770 173921_at (11)
NM_182235 173921_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5867243-5870817(-) 99.42 99.42
Public Domain and Genome References
Ensembl F55C12.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK73879 Hypothetical protein F55C12.5b [Caenorhabditis elegans] ref|NP_495171.1| F55C12.5b [Caenorhabditis elegans] 0.0
blast T34308 hypothetical protein F55C12.5 - Caenorhabditis elegans 0.0
blast AAK73878 Hypothetical protein F55C12.5a [Caenorhabditis elegans] ref|NP_495172.1| F55C12.5a [Caenorhabditis elegans] 0.0
blast AAN39671 Hypothetical protein F55C12.5c [Caenorhabditis elegans] ref|NP_872035.1| F55C12.5c [Caenorhabditis elegans] 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495171.1 1 225-247
NP_872035.1 1 225-247
F55C12.5b 1 225-247
F55C12.5c 1 225-247
Sequence
Target Sequence 
>C. ELEGANS:173921_AT
gactattcaagtcttttttcacaaaatgtttgtatattattttgcgttttcgtgcatagg
cattattactcattttgaattcatctttttatttttcgccttttattttccaaatcttca
caacggtgattttctttgttgttgatagcttatgatgttccgattaccattccattttta
ctttgagacattatttttatttgacatctcctcgtcactctccctttattgctttaaact
cccaactgaaatgttctccacgattttattttctttccttttattgcacatcctcaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTATTCAAGTCTTTTTTCACAAA 256 371 2705 Antisense
TTGTATATTATTTTGCGTTTTCGTG 599 707 2734 Antisense
GTGCATAGGCATTATTACTCATTTT 310 481 2756 Antisense
ATTTTCCAAATCTTCACAACGGTGA 457 13 2809 Antisense
ATGATGTTCCGATTACCATTCCATT 202 47 2856 Antisense
CCATTCCATTTTTACTTTGAGACAT 648 269 2871 Antisense
TTTATTTGACATCTCCTCGTCACTC 194 675 2900 Antisense
CACTCTCCCTTTATTGCTTTAAACT 55 199 2920 Antisense
ACTCCCAACTGAAATGTTCTCCACG 179 99 2942 Antisense
TCTCCACGATTTTATTTTCTTTCCT 564 609 2959 Antisense
TTTCCTTTTATTGCACATCCTCAAT 599 669 2978 Antisense
Sequence Source Affymetrix Proprietary Database