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[173917_at] guanine-nucleotide releasing factor

Description CEK025C5R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F07C3.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D33037 /WB_GENE_ID=F07C3.4 /WP=CE07026 /CHR=5 /FEA=Genomic Cluster /DEF=guanine-nucleotide releasing factor; C.elegans cDNA clone yk25c5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 77      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 97      6grp_total > 68      4grp_total > 13      6grp_highest_sub > Da      6grp_cgc > 48      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1405
1 2 3 4 range
A 1006.2
(1)
P 		416.6
(-2.7)
P 		374.7
(-3)
P 		1323.6
(3)
P 		949
B 676.1
(-1.5)
P 		583.2
(-1.9)
P 		315.2
(-3)
P 		1720.6
(3)
P 		1405
C 1052.3
(-0.2)
P 		510.9
(-2.9)
P 		492.5
(-3)
P 		1676.5
(3)
P 		1184
avr 911.5
(-0.4) 		503.6
(-2.4) 		394.1
(-3) 		1573.6
(3) 		1180

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 897
1 2 3 4 5 6 range
A 1276.9
(3)
P 		861.8
(-1.3)
P 		960.6
(-0.2)
P 		691.8
(-3)
P 		1074.8
(0.9)
P 		1071.6
(0.9)
P 		585
B 737.2
(-3)
P 		844.4
(-2.2)
P 		779.7
(-2.7)
P 		1207.5
(0.5)
P 		861.9
(-2.1)
P 		1532.1
(3)
P 		795
C 746.6
(-2.2)
P 		990.1
(-0.4)
P 		634.7
(-3)
P 		1002.3
(-0.3)
P 		881.4
(-1.2)
P 		1441.6
(3)
P 		807
avr 920.2
(-1.6) 		898.8
(-1.8) 		791.7
(-3) 		967.2
(-1.1) 		939.4
(-1.4) 		1348.4
(3) 		557
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 294
1 2 3 4 range
A 366.3
(3)
P 		90.4
(-3)
P 		98
(-2.8)
P 		193.7
(-0.8)
P 		276
B 215.6
(-0.1)
P 		144
(-1.5)
P 		73
(-3)
P 		366.7
(3)
P 		294
C 348.3
(3)
P 		146.9
(-3)
P 		171.7
(-2.3)
P 		282.7
(1)
P 		201
avr 310.1
(3) 		127.1
(-2.6) 		114.2
(-3) 		281
(2.1) 		196

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 410
1 2 3 4 5 6 range
A 235.4
(-0.6)
P 		306.5
(2.3)
P 		209.2
(-1.6)
P 		175.5
(-3)
P 		324.3
(3)
P 		260.9
(0.4)
P 		149
B 256.5
(-1.9)
P 		281.1
(-1.6)
P 		189.3
(-3)
P 		319.8
(-1)
P 		350.2
(-0.5)
P 		571.3
(3)
P 		382
C 276.7
(-0.8)
P 		302.1
(-0.4)
P 		161.5
(-3)
P 		201.6
(-2.3)
P 		285.2
(-0.7)
P 		482.6
(3)
P 		321
avr 256.2
(-1.3) 		296.6
(-0.4) 		186.7
(-3) 		232.3
(-1.9) 		319.9
(0.2) 		438.3
(3) 		252
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173917_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25186
Sequence Type Exemplar sequence
Representative Public ID CEK025C5R NCBI
Target Description CEK025C5R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F07C3.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D33037 /WB_GENE_ID=F07C3.4 /WP=CE07026 /CHR=5 /FEA=Genomic Cluster /DEF=guanine-nucleotide releasing factor; C.elegans cDNA clone yk25c5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072991(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072991 NCBI Caenorhabditis elegans Gut granule LOss family member (glo-4) (glo-4) mRNA, complete cds. 11/11 None
F07C3.4 ENSEMBL cdna:known chromosome:CEL140:V:9237209:9245749:-1 gene:F07C3.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9237219-9243123(-) 100.0 100.0
Public Domain and Genome References
Gene Title guanine-nucleotide releasing factor
Gene Symbol glo-4
Ensembl F07C3.4
Entrez Gene 179306 Entrez gene
SwissProt Q5DX34 EMBL-EBI
WormBase CE38062 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_505392 Gut granule LOss family member (glo-4) [Caenorhabditis elegans] gb|AAW88404.1| Hypothetical protein F07C3.4 [Caenorhabditis elegans] sp|Q5DX34|RPGRH_CAEEL X-linked retinitis pigmentosa GTPase regulator homolog 0.0
blast CAE72137 Hypothetical protein CBG19235 [Caenorhabditis briggsae] 0.0
blast CAE72138 Hypothetical protein CBG19236 [Caenorhabditis briggsae] 1.0E-123
Protein Domains
Database ID Description E-Value
Pfam IPR000408 EMBL-EBI Regulator of chromosome condensation, RCC1 2.9E-7
Sequence
Target Sequence 
>C. ELEGANS:173917_AT
cattttgatcatctagaccccttttgcctttaccctttacaatttaactatccattttta
ccggcgattttacgggttttctattccctcatgtcccaatgagcactttccgtgtttttt
tatttgcactcaatgatctaacagtccacctatcaaccatgctcaaaacatcaaactatg
gccttcaattttctcggtttgaaacgtggtaaatgtctgaatttatgtgttctagaggcc
ttgtttcctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATTTTGATCATCTAGACCCCTTTT 330 213 3382 Antisense
TACCCTTTACAATTTAACTATCCAT 531 647 3412 Antisense
TAACTATCCATTTTTACCGGCGATT 81 637 3426 Antisense
TTTACGGGTTTTCTATTCCCTCATG 55 643 3450 Antisense
ATTCCCTCATGTCCCAATGAGCACT 295 9 3464 Antisense
CAATGAGCACTTTCCGTGTTTTTTT 685 181 3478 Antisense
GTGTTTTTTTATTTGCACTCAATGA 148 485 3493 Antisense
CTCAATGATCTAACAGTCCACCTAT 301 227 3510 Antisense
CACCTATCAACCATGCTCAAAACAT 591 201 3528 Antisense
AACTATGGCCTTCAATTTTCTCGGT 652 139 3555 Antisense
TGTGTTCTAGAGGCCTTGTTTCCTT 234 555 3607 Antisense
Sequence Source Affymetrix Proprietary Database