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[173901_s_at] isoleucyl-tRNA synthetase (mitochondrial)

Description C65140 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C25A1.7A /5_PRIME_EXT_DB=WormBase Gene ID /GB=C65140 /WB_GENE_ID=C25A1.7A /WP=CE08372 /CHR=1 /FEA=Genomic Cluster /DEF=isoleucyl-tRNA synthetase (mitochondrial); C.elegans cDNA clone yk408e2 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 54      4grp_highest_8_8 > 35      4grp_high_8_8 > 48      6grp_highest_nonorm > 81      6grp_total > 45      4grp_total > 55      6grp_cgc > 55      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 142
1 2 3 4 range
A 315.3
(3)
P 		259.2
(-3)
P 		291.8
(0.5)
P 		271.7
(-1.7)
P 		56
B 328.8
(3)
P 		248.2
(-1.6)
P 		318.8
(2.4)
P 		223
(-3)
P 		106
C 364.9
(3)
P 		272.5
(-3)
P 		272.5
(-3)
P 		287.2
(-2)
P 		92
avr 336.3
(3) 		260
(-3) 		294.4
(-0.3) 		260.6
(-3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 218
1 2 3 4 5 6 range
A 195.8
(-3)
P 		258.7
(1)
P 		291.1
(3)
P 		223.1
(-1.3)
P 		209.4
(-2.1)
P 		208
(-2.2)
P 		95
B 306.9
(0.9)
P 		282.2
(0.1)
P 		370.2
(3)
P 		246.7
(-1.1)
P 		189.3
(-3)
P 		349.1
(2.3)
P 		181
C 217
(-1.8)
P 		392.2
(3)
P 		267.1
(-0.4)
P 		274.5
(-0.2)
P 		174
(-3)
P 		269.8
(-0.4)
P 		218
avr 239.9
(-0.6) 		311
(3) 		309.5
(2.9) 		248.1
(-0.1) 		190.9
(-3) 		275.6
(1.2) 		120
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 81
1 2 3 4 range
A 114.8
(3)
P 		56.3
(-1.7)
P 		76.3
(-0.1)
P 		39.8
(-3)
P 		75
B 104.8
(3)
P 		61.3
(-1.6)
P 		73.9
(-0.2)
P 		47.5
(-3)
P 		57
C 120.8
(3)
P 		78.4
(-0.5)
P 		95
(0.9)
P 		48.4
(-3)
P 		72
avr 113.5
(3) 		65.3
(-1.2) 		81.7
(0.2) 		45.2
(-3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 94
1 2 3 4 5 6 range
A 36.1
(-3)
P 		92
(3)
P 		63.4
(-0.1)
P 		56.6
(-0.8)
P 		63.2
(-0.1)
P 		50.7
(-1.4)
P 		56
B 106.8
(0.8)
P 		93.9
(-0.4)
P 		89.9
(-0.7)
P 		65.3
(-3)
P 		76.9
(-1.9)
P 		130.2
(3)
P 		65
C 80.4
(-0.7)
P 		119.7
(3)
P 		68
(-1.8)
P 		55.2
(-3)
P 		56.3
(-2.9)
P 		90.3
(0.3)
P 		65
avr 74.4
(-0.8) 		101.9
(3) 		73.8
(-0.9) 		59
(-3) 		65.5
(-2.1) 		90.4
(1.4) 		43
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173901_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22616
Sequence Type Exemplar sequence
Representative Public ID C65140 NCBI
Target Description C65140 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C25A1.7A /5_PRIME_EXT_DB=WormBase Gene ID /GB=C65140 /WB_GENE_ID=C25A1.7A /WP=CE08372 /CHR=1 /FEA=Genomic Cluster /DEF=isoleucyl-tRNA synthetase (mitochondrial); C.elegans cDNA clone yk408e2 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025836
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000000668 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10187966:10192053:1 None
C25A1.7.2 ENSEMBL cdna:known chromosome:CEL140:I:10187961:10192116:1 gene:C25A1.7 A 
C25A1.7.1 ENSEMBL cdna:known chromosome:CEL140:I:10187961:10192105:1 gene:C25A1.7 A 
C25A1.7.3 ENSEMBL cdna:known chromosome:CEL140:I:10188048:10192105:1 gene:C25A1.7 None
SNAP00000000656 ENSEMBL cdna:SNAP chromosome:CEL140:I:10187966:10192053:1 None
NM_001025836 NCBI Caenorhabditis elegans Isoleucyl tRNA Synthetase family member (irs-2) (irs-2) mRNA, complete cds. None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000010995 1/11 Cross Hyb Matching Probes None
Y38C1AA.12 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10187897-10192276(+) 100.0 100.0
Public Domain and Genome References
Gene Title isoleucyl-tRNA synthetase (mitochondrial)
Gene Symbol irs-2
Ensembl C25A1.7
Entrez Gene 172884 Entrez gene
SwissProt O02222 EMBL-EBI
WormBase CE35957 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:248634_AT tRNA synthetase class I (I, L, M and V) family protein at
CANINE:1602811_AT similar to mitochondrial isoleucine tRNA synthetase cfa
CANINE_2:CFA.15958.1.S1_AT similar to mitochondrial isoleucine tRNA synthetase cfa
CANINE_2:CFA.18217.1.S1_S_AT similar to mitochondrial isoleucine tRNA synthetase cfa
CANINE_2:CFA.19161.1.S1_S_AT similar to mitochondrial isoleucine tRNA synthetase cfa
CANINE_2:CFA.19161.2.S1_S_AT similar to mitochondrial isoleucine tRNA synthetase cfa
DROSOPHILA_2:1633557_AT dm
DROSGENOME1:148847_AT dm
CHICKEN:GGA.8402.1.S1_S_AT isoleucine-tRNA synthetase 2, mitochondrial gga
CHICKEN:GGA.8402.2.S1_AT isoleucine-tRNA synthetase 2, mitochondrial gga
HG-U133_PLUS_2:217900_AT isoleucine-tRNA synthetase 2, mitochondrial hs
HG-U133A:217900_AT isoleucine-tRNA synthetase 2, mitochondrial hs
HG-U133A_2:217900_AT isoleucine-tRNA synthetase 2, mitochondrial hs
HG-U95AV2:36647_AT isoleucine-tRNA synthetase 2, mitochondrial hs
HU35KSUBA:RC_AA236516_AT isoleucine-tRNA synthetase 2, mitochondrial hs
U133_X3P:G8922355_3P_AT isoleucine-tRNA synthetase 2, mitochondrial hs
HG-U95C:63913_AT isoleucine-tRNA synthetase 2, mitochondrial hs
HU35KSUBA:RC_AA428121_R_AT Isoleucine-tRNA synthetase 2, mitochondrial hs
HU35KSUBA:AA293868_S_AT Isoleucine-tRNA synthetase 2, mitochondrial hs
U133_X3P:HS.197289.0.A3_3P_AT Isoleucine-tRNA synthetase 2, mitochondrial hs
HG-U133A_2:202372_AT Isoleucine-tRNA synthetase 2, mitochondrial hs
HG-U133_PLUS_2:202372_AT Isoleucine-tRNA synthetase 2, mitochondrial hs
HG-U133A:202372_AT Isoleucine-tRNA synthetase 2, mitochondrial hs
HG-U95AV2:32763_R_AT Isoleucine-tRNA synthetase 2, mitochondrial hs
HG-U95B:53864_AT Isoleucine-tRNA synthetase 2, mitochondrial hs
HG-U95B:47291_AT Isoleucine-tRNA synthetase 2, mitochondrial hs
HU35KSUBC:RC_AA297739_AT Isoleucine-tRNA synthetase 2, mitochondrial hs
MU11KSUBB:MSA.3490.0_S_AT isoleucine-tRNA synthetase 2, mitochondrial mm
MU11KSUBB:W18438_S_AT isoleucine-tRNA synthetase 2, mitochondrial mm
MG-U74AV2:97422_AT isoleucine-tRNA synthetase 2, mitochondrial mm
MG-U74BV2:110536_AT isoleucine-tRNA synthetase 2, mitochondrial mm
MOE430A:1426735_AT isoleucine-tRNA synthetase 2, mitochondrial mm
MOUSE430A_2:1426735_AT isoleucine-tRNA synthetase 2, mitochondrial mm
MOUSE430_2:1426735_AT isoleucine-tRNA synthetase 2, mitochondrial mm
MOE430B:1441665_AT isoleucine-tRNA synthetase 2, mitochondrial mm
MOUSE430_2:1441665_AT isoleucine-tRNA synthetase 2, mitochondrial mm
MU19KSUBB:TC26055_AT Isoleucine-tRNA synthetase 2, mitochondrial, mRNA (cDNA clone IMAGE:3982770) mm
MU19KSUBB:TC26055_G_AT Isoleucine-tRNA synthetase 2, mitochondrial, mRNA (cDNA clone IMAGE:3982770) mm
MU19KSUBB:TC29867_AT Isoleucine-tRNA synthetase 2, mitochondrial, mRNA (cDNA clone IMAGE:3982770) mm
MU19KSUBC:TC35460_AT Isoleucine-tRNA synthetase 2, mitochondrial, mRNA (cDNA clone IMAGE:3982770) mm
MU19KSUBC:TC36295_AT Isoleucine-tRNA synthetase 2, mitochondrial, mRNA (cDNA clone IMAGE:3982770) mm
RAE230B:1380561_AT similar to hypothetical protein FLJ10326 (predicted) rn
RAT230_2:1380561_AT similar to hypothetical protein FLJ10326 (predicted) rn
RAE230A:1374609_AT Similar to hypothetical protein FLJ10326 (predicted) rn
RAT230_2:1374609_AT Similar to hypothetical protein FLJ10326 (predicted) rn
RAE230A:1376817_AT Similar to hypothetical protein FLJ10326 (predicted) rn
RAT230_2:1376817_AT Similar to hypothetical protein FLJ10326 (predicted) rn
RG-U34B:RC_AI011183_AT Similar to hypothetical protein FLJ10326 (predicted) rn
RG-U34B:RC_AI058285_AT Similar to hypothetical protein FLJ10326 (predicted) rn
YEAST_2:1776686_AT Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6418 tRNA aminoacylation for protein translation inferred from electronic annotation QuickGO AmiGO
6428 isoleucyl-tRNA aminoacylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4812 tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
4822 isoleucine-tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02766 Hypothetical protein C25A1.7 [Caenorhabditis elegans] ref|NP_001021007.1| Isoleucyl tRNA Synthetase family member (irs-2) [Caenorhabditis elegans] 0.0
blast BAC76731.1 isoleucyl-tRNA synthetase (mitochondrial) [Caenorhabditis elegans] 0.0
blast CAE66853 Hypothetical protein CBG12225 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002300 EMBL-EBI Aminoacyl-tRNA synthetase, class Ia 3.2E-80
Pfam IPR002300 EMBL-EBI Aminoacyl-tRNA synthetase, class Ia 1.6E-47
Pfam IPR002300 EMBL-EBI Aminoacyl-tRNA synthetase, class Ia 3.2E-80
Pfam IPR002300 EMBL-EBI Aminoacyl-tRNA synthetase, class Ia 1.6E-47
Pfam IPR002300 EMBL-EBI Aminoacyl-tRNA synthetase, class Ia 3.2E-80
Pfam IPR002300 EMBL-EBI Aminoacyl-tRNA synthetase, class Ia 1.6E-47
Sequence
Target Sequence 
>C. ELEGANS:173901_S_AT
aagatcttcattatggacttctcactccacatgatcagctttcaaagcacatgaaattcg
tatacgaagttcgaaatcttttagagtcaactgctggtccaaagattgatacttctaaaa
aaggaattgttcttcaagtgacaccagaataccacacaattcttcgcatttacgaatcag
agctgccagaattgttaaatgtttcacaagtgatactgcatgaaaatcgggatgaaaatt
ctgttgagctcgtggaaagccggttgcgatattgtgagagatgccggaagcacactcgga
atgaaagtgatgctctatgcggaagatgctcagatgcgatgggactttctcgcgccacgt
tttcaagatctgttcaaaaacaagatttacgaaaacaagatccaccaaaacaagatctac
attttcaagatctaccaaaacaagatctacgttttcaagatctggcaccgtgccaactgt
ggttttctcgaagaaaaccaaacaacgatgctccgatgttacgcgtcgcgtgttgtttag
cgttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGATCTTCATTATGGACTTCTCAC 429 155 2833 Antisense
TCACTCCACATGATCAGCTTTCAAA 12 627 2854 Antisense
TAGAGTCAACTGCTGGTCCAAAGAT 487 637 2914 Antisense
ATACCACACAATTCTTCGCATTTAC 102 23 2981 Antisense
GAGCTCGTGGAAAGCCGGTTGCGAT 216 387 3078 Antisense
GAGAGATGCCGGAAGCACACTCGGA 39 395 3108 Antisense
GATGCTCTATGCGGAAGATGCTCAG 41 415 3141 Antisense
AGATGCGATGGGACTTTCTCGCGCC 263 67 3164 Antisense
GTGCCAACTGTGGTTTTCTCGAAGA 60 479 3302 Antisense
ACAACGATGCTCCGATGTTACGCGT 526 113 3334 Antisense
CGCGTCGCGTGTTGTTTAGCGTTCA 456 253 3354 Antisense
Sequence Source Affymetrix Proprietary Database