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[173888_at] phosphatidylinositol 3-kinase like

Description CEK017B8R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F39B1.1 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D32461 /WB_GENE_ID=F39B1.1 /WP=CE05832 /CHR=X /FEA=Genomic Cluster /DEF=phosphatidylinositol 3-kinase like; C.elegans cDNA clone yk17b8 : 3prime end, single read.
Cluster 6grp_high1_10_10 > 07      4grp_high_8_8 > 10      6grp_total > 05      4grp_total > 22      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 87.9
(2.6)
P 		41.3
(-2.5)
P 		36.3
(-3)
A 		91.4
(3)
P 		55
B 54
(3)
P 		44.5
(0.5)
P 		31.5
(-3)
A 		43.9
(0.3)
P 		23
C 71
(2)
P 		48.3
(-1)
P 		32.7
(-3)
P 		78.4
(3)
P 		46
avr 71
(3) 		44.7
(-1.2) 		33.5
(-3) 		71.2
(3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 66.2
(2.8)
P 		67.7
(3)
P 		48.4
(0.3)
P 		24.4
(-3)
A 		60.7
(2)
P 		29.3
(-2.3)
A 		43
B 72.5
(2.4)
P 		76.5
(3)
P 		55.4
(0)
M 		54.7
(-0)
P 		33.6
(-3)
P 		43.2
(-1.7)
P 		43
C 91.7
(3)
P 		46.8
(-0.8)
P 		19.9
(-3)
P 		36.6
(-1.6)
A 		37.6
(-1.5)
P 		33.6
(-1.9)
A 		72
avr 76.8
(3) 		63.7
(1.1) 		41.2
(-2.2) 		38.6
(-2.5) 		44
(-1.8) 		35.4
(-3) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 32
(3)
P 		9
(-3)
P 		9.5
(-2.9)
A 		13.4
(-1.9)
P 		23
B 17.2
(3)
P 		11
(-0.8)
P 		7.3
(-3)
A 		9.4
(-1.7)
P 		10
C 23.5
(3)
P 		13.9
(-1.8)
P 		11.4
(-3)
P 		13.2
(-2.1)
P 		12
avr 24.2
(3) 		11.3
(-2.2) 		9.4
(-3) 		12
(-1.9) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 12.2
(-1)
P 		24.1
(3)
P 		10.5
(-1.6)
P 		6.2
(-3)
A 		18.3
(1.1)
P 		7.1
(-2.7)
A 		18
B 25.2
(2.9)
P 		25.5
(3)
P 		13.5
(-3)
M 		14.5
(-2.5)
P 		13.6
(-3)
P 		16.1
(-1.7)
P 		12
C 34
(3)
P 		14.3
(-1.1)
P 		5.1
(-3)
P 		7.4
(-2.5)
A 		12.2
(-1.5)
P 		11.2
(-1.7)
A 		29
avr 23.8
(3) 		21.3
(2) 		9.7
(-2.9) 		9.4
(-3) 		14.7
(-0.8) 		11.5
(-2.1) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173888_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26077
Sequence Type Exemplar sequence
Representative Public ID CEK017B8R NCBI
Target Description CEK017B8R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F39B1.1 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D32461 /WB_GENE_ID=F39B1.1 /WP=CE05832 /CHR=X /FEA=Genomic Cluster /DEF=phosphatidylinositol 3-kinase like; C.elegans cDNA clone yk17b8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078128(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078128 NCBI Caenorhabditis elegans F39B1.1 (F39B1.1) mRNA, complete cds. 11/11 None
F39B1.1 ENSEMBL cdna:known chromosome:CEL140:X:15234764:15246282:-1 gene:F39B1.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:15234774-15246283(-) 100.0 100.0
Public Domain and Genome References
Gene Title phosphatidylinositol 3-kinase like
Ensembl F39B1.1
Entrez Gene 181618 Entrez gene
SwissProt Q20187 EMBL-EBI
WormBase CE05832 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.13770.1.S1_S_AT similar to phosphoinositide-3-kinase, class 2, alpha polypeptide cfa
DROSOPHILA_2:1625233_AT Phosphotidylinositol 3 kinase 68D dm
DROSOPHILA_2:1641006_S_AT Phosphotidylinositol 3 kinase 68D dm
DROSGENOME1:151675_S_AT Phosphotidylinositol 3 kinase 68D dm
DROSGENOME1:142750_AT Phosphotidylinositol 3 kinase 68D dm
CHICKEN:GGA.12762.1.S1_AT similar to phosphoinositide-3-kinase, class 2, alpha polypeptide; C2-containing phosphatidylinositol kinase; PI3K-C2alpha gga
CHICKEN:GGAAFFX.26752.1.S1_S_AT similar to phosphoinositide-3-kinase, class 2, alpha polypeptide; C2-containing phosphatidylinositol kinase; PI3K-C2alpha gga
HG-U133_PLUS_2:1553694_A_AT phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U133_PLUS_2:1569022_A_AT phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U133A:213070_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U133A_2:213070_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U133_PLUS_2:213070_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HU35KSUBB:RC_H13179_S_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
U133_X3P:HS2.249235.2.S1_3P_X_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
U133_X3P:HS2.249235.2.S1_3P_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
U133_X3P:HS2.249235.1.S1_3P_S_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
U133_X3P:HS.5848.0.S1_3P_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
U133_X3P:HS.259718.0.S1_3P_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
U133_X3P:HS.249235.0.S2_3P_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
U133_X3P:HS.207212.0.A1_3P_S_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
U133_X3P:HS.207212.0.A1_3P_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
U133_X3P:235792_3P_X_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U133_PLUS_2:1569021_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U133_PLUS_2:226094_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U133B:226094_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U133_PLUS_2:235792_X_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U133B:235792_X_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U133_PLUS_2:241905_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U133B:241905_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U95AV2:36509_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U95E:76325_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U95E:77866_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U95E:86523_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U95E:68966_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
HG-U95E:77869_S_AT Phosphoinositide-3-kinase, class 2, alpha polypeptide hs
MG-U74AV2:92312_AT phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide mm
MOUSE430_2:1425862_A_AT phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide mm
MOE430A:1425862_A_AT phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide mm
MOUSE430A_2:1425862_A_AT phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide mm
MU11KSUBA:U52193_S_AT phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide mm
MG-U74AV2:92311_S_AT phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide mm
MOE430A:1421023_AT phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide mm
MOUSE430A_2:1421023_AT phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide mm
MOUSE430_2:1421023_AT phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide mm
MOE430B:1443655_S_AT phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide mm
MOUSE430_2:1443655_S_AT phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide mm
MG-U74BV2:111831_AT Phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide (Pik3c2a), mRNA mm
MG-U74CV2:140944_AT Phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide (Pik3c2a), mRNA mm
MU19KSUBC:TC35015_AT Phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide (Pik3c2a), mRNA mm
MU19KSUBC:TC35421_AT Phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide (Pik3c2a), mRNA mm
RAE230B:1379433_AT Phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide (predicted) rn
RAT230_2:1379433_AT Phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide (predicted) rn
RG-U34B:RC_AI013939_AT Phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide (predicted) rn
RG-U34B:RC_AI059449_AT Phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5942 phosphoinositide 3-kinase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4428 inositol or phosphatidylinositol kinase activity inferred from electronic annotation QuickGO AmiGO
16303 phosphatidylinositol 3-kinase activity inferred from electronic annotation QuickGO AmiGO
16773 phosphotransferase activity, alcohol group as acceptor inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA93776 Hypothetical protein F39B1.1 [Caenorhabditis elegans] emb|CAA93489.1| Hypothetical protein F39B1.1 [Caenorhabditis elegans] ref|NP_510529.1| F39B1.1 [Caenorhabditis elegans] 0.0
blast CAE63290 Hypothetical protein CBG07670 [Caenorhabditis briggsae] 0.0
blast XP_396869 PREDICTED: similar to GA11104-PA [Apis mellifera] 1.0E-163
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 3.1E-17
Pfam IPR000403 EMBL-EBI Phosphatidylinositol 3- and 4-kinase 6.2E-86
Pfam IPR003903 EMBL-EBI Ubiquitin interacting motif 0.0014
Pfam IPR001683 EMBL-EBI Phox-like 3.1E-24
Pfam IPR002420 EMBL-EBI Phosphoinositide 3-kinase, C2 domain 1.0E-34
Pfam IPR001263 EMBL-EBI Phosphoinositide 3-kinase accessory domain PIK 2.5E-17
Sequence
Target Sequence 
>C. ELEGANS:173888_AT
atgctggtatatttttttgttttgtttttcaaaatttatttcggatatgtatatatgttt
attatttggactttttacttatccaattttttactgagttttccacaacaccgtcatttt
ccatttcctttcatctgtgatatagtattttactccaaatccaaacagtacgaatacttg
ttagttttcaactttcagtttatcacaaagtctagtcaattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCTGGTATATTTTTTTGTTTTGT 45 41 4850 Antisense
TATTTGGACTTTTTACTTATCCAAT 597 659 4912 Antisense
TTTACTTATCCAATTTTTTACTGAG 447 673 4923 Antisense
TTTTACTGAGTTTTCCACAACACCG 585 671 4938 Antisense
TTTCCATTTCCTTTCATCTGTGATA 612 669 4967 Antisense
ATTTCCTTTCATCTGTGATATAGTA 472 17 4972 Antisense
GATATAGTATTTTACTCCAAATCCA 492 423 4988 Antisense
CTCCAAATCCAAACAGTACGAATAC 545 193 5002 Antisense
TACGAATACTTGTTAGTTTTCAACT 595 647 5018 Antisense
GTTTTCAACTTTCAGTTTATCACAA 138 449 5033 Antisense
GTTTATCACAAAGTCTAGTCAATTC 518 449 5047 Antisense
Sequence Source Affymetrix Proprietary Database