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[173880_s_at] myosin heavy chain

Description CEK030B1R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=R06C7.10 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D33383 /WB_GENE_ID=R06C7.10 /WP=CE06253 /CHR=1 /FEA=Genomic Cluster /GEN=myo-1 /DEF=myosin heavy chain; C.elegans cDNA clone yk30b1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 15      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 04      6grp_total > 15      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5936
1 2 3 4 range
A 7552.9
(3)
P 		3406.4
(-2.5)
P 		3036.7
(-3)
P 		3991.3
(-1.7)
P 		4516
B 8509.3
(3)
P 		3845.3
(-1.7)
P 		2573.4
(-3)
P 		3418.5
(-2.1)
P 		5936
C 7556.4
(3)
P 		2912.2
(-3)
P 		3010.7
(-2.9)
P 		3102.2
(-2.8)
P 		4644
avr 7872.9
(3) 		3388
(-2.4) 		2873.6
(-3) 		3504
(-2.2) 		4999

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6195
1 2 3 4 5 6 range
A 7114.8
(3)
P 		6120.6
(2)
P 		2556.3
(-1.7)
P 		2377.4
(-1.9)
P 		2157
(-2.2)
P 		1341.7
(-3)
P 		5773
B 7238.4
(3)
P 		5793.7
(1.6)
P 		2571.3
(-1.5)
P 		2682
(-1.4)
P 		1461.3
(-2.6)
P 		1043.4
(-3)
P 		6195
C 6486.7
(3)
P 		5393.9
(1.8)
P 		4115.4
(0.3)
P 		2210.1
(-1.8)
P 		1885.4
(-2.2)
P 		1188.6
(-3)
P 		5298
avr 6946.6
(3) 		5769.4
(1.8) 		3081
(-1) 		2423.2
(-1.7) 		1834.6
(-2.3) 		1191.2
(-3) 		5755
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2227
1 2 3 4 range
A 2750
(3)
P 		739.4
(-2.6)
P 		793.9
(-2.4)
P 		584.2
(-3)
P 		2166
B 2713.6
(3)
P 		949.7
(-2)
P 		596.3
(-3)
P 		728.5
(-2.6)
P 		2117
C 2501.4
(3)
P 		837.3
(-2)
P 		1049.3
(-1.4)
P 		523.1
(-3)
P 		1978
avr 2655
(3) 		842.1
(-2.3) 		813.2
(-2.4) 		611.9
(-3) 		2043

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2192
1 2 3 4 5 6 range
A 1311.6
(0.2)
P 		2176.8
(3)
P 		556.7
(-2.3)
P 		603
(-2.1)
P 		650.9
(-1.9)
P 		326.7
(-3)
P 		1850
B 2518.6
(3)
P 		1928.5
(1.3)
P 		624.4
(-2.3)
P 		710.4
(-2.1)
P 		593.7
(-2.4)
P 		389.1
(-3)
P 		2130
C 2404.3
(3)
P 		1645.8
(0.7)
P 		1047.4
(-1.1)
P 		444.5
(-2.9)
P 		610.1
(-2.4)
P 		397.9
(-3)
P 		2006
avr 2078.2
(3) 		1917
(2.4) 		742.8
(-1.7) 		586
(-2.2) 		618.2
(-2.1) 		371.2
(-3) 		1707
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173880_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23027
Sequence Type Exemplar sequence
Representative Public ID CEK030B1R NCBI
Target Description CEK030B1R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=R06C7.10 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D33383 /WB_GENE_ID=R06C7.10 /WP=CE06253 /CHR=1 /FEA=Genomic Cluster /GEN=myo-1 /DEF=myosin heavy chain; C.elegans cDNA clone yk30b1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059652(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059652 NCBI Caenorhabditis elegans LEThal family member (let-75) (let-75) mRNA, complete cds. 11/11 None
R06C7.10.2 ENSEMBL cdna:known chromosome:CEL140:I:7267035:7275210:-1 gene:R06C7.10 11/11 A 
R06C7.10.1 ENSEMBL cdna:known chromosome:CEL140:I:7267046:7275210:-1 gene:R06C7.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7267009-7274998(-) 100.0 100.0
Public Domain and Genome References
Gene Title myosin heavy chain
Gene Symbol let-75
Ensembl R06C7.10
Entrez Gene 172471 Entrez gene
SwissProt P02567 EMBL-EBI
WormBase CE06253 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.17595.1.S1_S_AT similar to myosin, heavy polypeptide 7B, cardiac muscle, beta cfa
CANINE_2:CFA.17613.1.S1_S_AT similar to myosin, heavy polypeptide 7B, cardiac muscle, beta cfa
CANINE_2:CFAAFFX.12636.1.S1_S_AT similar to myosin, heavy polypeptide 7B, cardiac muscle, beta cfa
CANINE_2:CFAAFFX.12665.1.S1_S_AT similar to myosin, heavy polypeptide 7B, cardiac muscle, beta cfa
CHICKEN:GGA.103.1.S1_AT myosin, heavy polypeptide 7B, cardiac muscle, beta gga
HG-U133_PLUS_2:233949_S_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
HG-U133B:233949_S_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
HG-U133_PLUS_2:215795_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
HG-U133A_2:215795_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
HG-FOCUS:215795_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
HG-U133A:215795_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
HU35KSUBA:HUM_ALU_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
HU35KSUBB:HUM_ALU_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
HU35KSUBC:HUM_ALU_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
HU35KSUBD:HUM_ALU_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
HUGENEFL:HUM_ALU_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
HC-G110:HUM_ALU_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
U133_X3P:HS.272207.1.S1_3P_X_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
U133_X3P:HS.272207.1.S1_3P_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
U133_X3P:HS.154788.1.S1_3P_A_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
U133_X3P:215795_3P_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
HG-U95E:83515_AT myosin, heavy polypeptide 7B, cardiac muscle, beta hs
U133_X3P:HS2.375849.1.A1_3P_AT Myosin, heavy polypeptide 7B, cardiac muscle, beta hs
HG-U133_PLUS_2:1557744_AT Myosin, heavy polypeptide 7B, cardiac muscle, beta hs
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16459 myosin inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3774 motor activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA95848 Hypothetical protein R06C7.10 [Caenorhabditis elegans] emb|CAA95806.1| Hypothetical protein R06C7.10 [Caenorhabditis elegans] ref|NP_492053.1| LEThal family member (let-75) [Caenorhabditis elegans] 0.0
blast CAA30854 myosin 1 [Caenorhabditis elegans] sp|P02567|MYO1_CAEEL Myosin-1 (Myosin heavy chain D) (MHC D) 0.0
blast CAE74227 Hypothetical protein CBG21911 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.7.Methicillin resistance protein FemA probable tRNA-binding arm All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Methicillin resistance protein FemA probable tRNA-binding arm 0.589999973773956
Pfam IPR004009 EMBL-EBI Myosin N-terminal SH3-like domain 1.1E-16
Pfam IPR002928 EMBL-EBI Myosin tail 1.0E-126
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:173880_S_AT
cagtctcaatcatattctctcactctctgtttgaacgattcgcgcgccgagtctccgttg
atctttaaattctgttgatttctcccgacgctccacacgtctgaaattgatataatctga
aagtctcgttgacatcataattctttcgattctagcgatcgaccctccacattatcaatc
agtaaactgccttccaaaagccccaggctctagaaaatcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGTCTCAATCATATTCTCTCACTC 670 207 6047 Antisense
TTCTCTCACTCTCTGTTTGAACGAT 676 691 6061 Antisense
TCTCTGTTTGAACGATTCGCGCGCC 539 609 6070 Antisense
GAGTCTCCGTTGATCTTTAAATTCT 612 399 6095 Antisense
TAAATTCTGTTGATTTCTCCCGACG 556 641 6112 Antisense
CCGACGCTCCACACGTCTGAAATTG 385 263 6131 Antisense
GTCTCGTTGACATCATAATTCTTTC 390 471 6169 Antisense
CTTTCGATTCTAGCGATCGACCCTC 16 223 6189 Antisense
GACCCTCCACATTATCAATCAGTAA 701 381 6207 Antisense
ATCAATCAGTAAACTGCCTTCCAAA 651 25 6220 Antisense
AAGCCCCAGGCTCTAGAAAATCAAA 93 149 6244 Antisense
Sequence Source Affymetrix Proprietary Database