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[173870_s_at] putative protein tyrosine kinase

Description CEK124B3R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F57B9.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D66820 /WB_GENE_ID=F57B9.8 /WP=CE01343 /CHR=3 /FEA=Genomic Cluster /DEF=putative protein tyrosine kinase; C.elegans cDNA clone yk124b3 : 3prime end, single read.
Cluster 6grp_total > 89      4grp_total > 46      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 29.3
(-0.3)
A 		15.3
(-3)
A 		45.9
(3)
A 		17.9
(-2.5)
A 		31
B 65.2
(3)
A 		61
(2.5)
A 		34.1
(-0.4)
A 		10.3
(-3)
A 		55
C 30.8
(-0.7)
A 		25.6
(-1.4)
A 		12.4
(-3)
A 		61
(3)
A 		49
avr 41.8
(3) 		34
(-0.9) 		30.8
(-2.5) 		29.7
(-3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 135
1 2 3 4 5 6 range
A 43
(-1.3)
A 		7.4
(-2.9)
A 		6
(-3)
A 		15.7
(-2.6)
A 		79.1
(0.3)
A 		138.5
(3)
A 		133
B 17.3
(-2.4)
A 		19.4
(-2.3)
A 		7.9
(-3)
A 		35.7
(-1.2)
A 		97.3
(2.8)
A 		101.1
(3)
A 		93
C 31.2
(-0.3)
A 		31.2
(-0.3)
A 		22.5
(-1.1)
A 		3.5
(-3)
A 		57.7
(2.3)
A 		64.6
(3)
A 		61
avr 30.5
(-1.8) 		19.3
(-2.5) 		12.1
(-3) 		18.3
(-2.6) 		78
(1.4) 		101.4
(3) 		89
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 10.7
(2.2)
A 		3.3
(-2.6)
A 		12
(3)
A 		2.6
(-3)
A 		9
B 20.8
(3)
A 		15.1
(1.2)
A 		7.9
(-1.2)
A 		2.2
(-3)
A 		19
C 10.2
(2.9)
A 		7.4
(0.1)
A 		4.3
(-3)
A 		10.3
(3)
A 		6
avr 13.9
(3) 		8.6
(-0.6) 		8.1
(-0.9) 		5
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 7.9
(-1.8)
A 		2.6
(-2.8)
A 		1.3
(-3)
A 		4
(-2.5)
A 		23.9
(1.2)
A 		33.7
(3)
A 		32
B 6
(-2.3)
A 		6.5
(-2.3)
A 		1.9
(-3)
A 		9.5
(-1.8)
A 		39.5
(3)
A 		37.7
(2.7)
A 		38
C 11.6
(0.1)
A 		9.5
(-0.5)
A 		5.7
(-1.6)
A 		0.7
(-3)
A 		18.7
(2.2)
A 		21.6
(3)
A 		21
avr 8.5
(-1.8) 		6.2
(-2.3) 		3
(-3) 		4.7
(-2.6) 		27.4
(2.2) 		31
(3) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173870_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23989
Sequence Type Exemplar sequence
Representative Public ID CEK124B3R NCBI
Target Description CEK124B3R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F57B9.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D66820 /WB_GENE_ID=F57B9.8 /WP=CE01343 /CHR=3 /FEA=Genomic Cluster /DEF=putative protein tyrosine kinase; C.elegans cDNA clone yk124b3 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066110(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066110 NCBI Caenorhabditis elegans F57B9.8 (F57B9.8) mRNA, complete cds. 11/11 None
SNAP00000019898 ENSEMBL cdna:SNAP chromosome:CEL140:III:6933642:6935209:-1 11/11 None
GENEFINDER00000019907 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6933642:6935209:-1 11/11 None
F57B9.8 ENSEMBL cdna:known chromosome:CEL140:III:6933608:6935209:-1 gene:F57B9.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6933617-6935209(-) 100.0 100.0
Public Domain and Genome References
Gene Title putative protein tyrosine kinase
Ensembl F57B9.8
Entrez Gene 175967 Entrez gene
SwissProt Q20933 EMBL-EBI
WormBase CE29411 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:18176_AT protein kinase family protein at
ATH1-121501:252991_AT protein kinase family protein at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA21175 Hypothetical protein F57B9.8 [Caenorhabditis elegans] ref|NP_498511.1| F57B9.8 [Caenorhabditis elegans] 0.0
blast CAE70048 Hypothetical protein CBG16480 [Caenorhabditis briggsae] 1.0E-161
blast CAE63903 Hypothetical protein CBG08474 [Caenorhabditis briggsae] 1.0E-94
Protein Families
Method ID Description E-Value
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 5.0E-67
Protein Domains
Database ID Description E-Value
Pfam IPR000980 EMBL-EBI SH2 motif 5.9E-21
Pfam IPR000719 EMBL-EBI Protein kinase 2.6E-72
Sequence
Target Sequence 
>C. ELEGANS:173870_S_AT
aagtctctgacttcggtttatctcgagttggaacggagtatcagatggatccgagcaaaa
gagttccaatcagatggctctcaccagaaacgatcgtttccttcctttatactccacaaa
ctgatgtgtttgctttctcgattctctgctgggaggtgatcgagaatggagctcagccgt
atccggagatgttggtggtgcaggtacatcagaaggttggacgcgatgattaccgtatgc
cgattagtcaaaaggctccagctatgcttgccgatgttatgaaaaagtgctggattcgcg
atcctcaacagcgccccactatgagccaagttgtccagatgatgtacgaaatcacaggaa
agaaagataaaacctcggtcaacaagtcgacaggacagctccgtgcaaaaatgatgtcta
ctggaccaatgtcagatccgatgagagataaaactgctaaaaagactggaagacgtaaca
agaagaaaccgtcaagcgcatcgaacggtccaggtggcaactctgggcctagttcgggtc
cattgtctggta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTCTCTGACTTCGGTTTATCTCG 22 157 816 Antisense
AACTGATGTGTTTGCTTTCTCGATT 249 143 934 Antisense
GAGCTCAGCCGTATCCGGAGATGTT 119 389 984 Antisense
GGACGCGATGATTACCGTATGCCGA 178 523 1034 Antisense
CTCCAGCTATGCTTGCCGATGTTAT 14 237 1071 Antisense
AAAGTGCTGGATTCGCGATCCTCAA 496 121 1099 Antisense
CCCCACTATGAGCCAAGTTGTCCAG 687 261 1129 Antisense
ACAAGTCGACAGGACAGCTCCGTGC 606 113 1197 Antisense
GAAGAAACCGTCAAGCGCATCGAAC 116 339 1297 Antisense
ACGGTCCAGGTGGCAACTCTGGGCC 17 97 1320 Antisense
CCTAGTTCGGGTCCATTGTCTGGTA 37 261 1343 Antisense
Sequence Source Affymetrix Proprietary Database