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[173868_s_at] glycine cleavage system H protein like

Description C43005 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F52A8.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C43005 /WB_GENE_ID=F52A8.5 /WP=CE05912 /CHR=1 /FEA=Genomic Cluster /DEF=glycine cleavage system H protein like; C.elegans cDNA clone yk333a3 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 52      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 25      6grp_total > 52      4grp_total > 57      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3363
1 2 3 4 range
A 2598.4
(-2.2)
P 		3898.6
(1.4)
P 		4503.3
(3)
P 		2297
(-3)
P 		2206
B 2821.1
(-1.9)
P 		4602
(1.3)
P 		5558.1
(3)
P 		2195.6
(-3)
P 		3363
C 2637.3
(-2.4)
P 		4223.3
(1.6)
P 		4766.9
(3)
P 		2420.5
(-3)
P 		2346
avr 2685.6
(-2.1) 		4241.3
(1.4) 		4942.8
(3) 		2304.4
(-3) 		2638

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7029
1 2 3 4 5 6 range
A 3575.5
(-2)
P 		4959.3
(-0.8)
P 		9462.4
(3)
P 		5942.4
(-0)
P 		3312.1
(-2.2)
P 		2433.4
(-3)
P 		7029
B 3798.7
(-1.8)
P 		6094.8
(0.6)
P 		8361.7
(3)
P 		6043.7
(0.5)
P 		3682.1
(-2)
P 		2701
(-3)
P 		5661
C 3229.2
(-2.7)
P 		5329.5
(1.5)
P 		5475.2
(1.8)
P 		6087.2
(3)
P 		3201.2
(-2.8)
P 		3085
(-3)
P 		3002
avr 3534.5
(-2.1) 		5461.2
(0.2) 		7766.4
(3) 		6024.4
(0.9) 		3398.5
(-2.2) 		2739.8
(-3) 		5027
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1325
1 2 3 4 range
A 946.1
(1.4)
P 		846.3
(0.6)
P 		1177.3
(3)
P 		336.2
(-3)
P 		841
B 899.6
(0.2)
P 		1136.6
(1.9)
P 		1287.9
(3)
P 		467.9
(-3)
P 		820
C 873
(-0.8)
P 		1214.2
(0.9)
P 		1661.4
(3)
P 		408.1
(-3)
P 		1253
avr 906.2
(0.1) 		1065.7
(1.1) 		1375.5
(3) 		404.1
(-3) 		971

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1468
1 2 3 4 5 6 range
A 659.1
(-2.7)
P 		1763.8
(1.8)
P 		2060.7
(3)
P 		1507.1
(0.7)
P 		999.5
(-1.3)
P 		592.5
(-3)
P 		1468
B 1321.8
(-1.2)
P 		2028.7
(3)
P 		2030.5
(3)
P 		1600.8
(0.5)
P 		1496
(-0.1)
P 		1007.2
(-3)
P 		1023
C 1196.9
(-1.3)
P 		1626.2
(3)
P 		1393.5
(0.6)
P 		1224.1
(-1.1)
P 		1035.9
(-3)
P 		1032.7
(-3)
P 		594
avr 1059.3
(-1.9) 		1806.2
(2.9) 		1828.2
(3) 		1444
(0.6) 		1177.1
(-1.1) 		877.5
(-3) 		951
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173868_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23062
Sequence Type Exemplar sequence
Representative Public ID C43005 NCBI
Target Description C43005 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F52A8.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C43005 /WB_GENE_ID=F52A8.5 /WP=CE05912 /CHR=1 /FEA=Genomic Cluster /DEF=glycine cleavage system H protein like; C.elegans cDNA clone yk333a3 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059674(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059674 NCBI Caenorhabditis elegans F52A8.5 (F52A8.5) mRNA, complete cds. 11/11 None
SNAP00000013993 ENSEMBL cdna:SNAP chromosome:CEL140:I:7354203:7354851:-1 11/11 None
GENEFINDER00000013995 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7354203:7354851:-1 11/11 None
F52A8.5.2 ENSEMBL cdna:known chromosome:CEL140:I:7354078:7354861:-1 gene:F52A8.5 11/11 None
F52A8.5.1 ENSEMBL cdna:known chromosome:CEL140:I:7354147:7355468:-1 gene:F52A8.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7354088-7354783(-) 100.0 100.0
Public Domain and Genome References
Gene Title glycine cleavage system H protein like
Ensembl F52A8.5
Entrez Gene 172486 Entrez gene
SwissProt Q20634 EMBL-EBI
WormBase CE05912 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:266517_AT glycine cleavage system H protein, mitochondrial, putative at
CANINE:1590648_AT similar to Glycine cleavage system H protein, mitochondrial precursor cfa
CANINE_2:CFA.8514.1.S1_AT similar to Glycine cleavage system H protein, mitochondrial precursor cfa
CANINE_2:CFA.8514.2.S1_S_AT similar to Glycine cleavage system H protein, mitochondrial precursor cfa
DROSOPHILA_2:1626366_AT pumpless dm
DROSGENOME1:141370_AT pumpless dm
CHICKEN:GGA.3298.1.S1_AT glycine cleavage system protein H (aminomethyl carrier) gga
HG-U95AV2:37357_AT glycine cleavage system protein H (aminomethyl carrier) hs
HU35KSUBD:RC_W92771_S_AT glycine cleavage system protein H (aminomethyl carrier) hs
HUGENEFL:D00723_AT glycine cleavage system protein H (aminomethyl carrier) hs
U133_X3P:HS.77631.1.A2_3P_A_AT glycine cleavage system protein H (aminomethyl carrier) hs
U133_X3P:HS.77631.1.A1_3P_A_AT glycine cleavage system protein H (aminomethyl carrier) hs
HG-U133A_2:213129_S_AT glycine cleavage system protein H (aminomethyl carrier) hs
HG-FOCUS:213129_S_AT glycine cleavage system protein H (aminomethyl carrier) hs
HG-U133_PLUS_2:213129_S_AT glycine cleavage system protein H (aminomethyl carrier) hs
HG-U133A:213129_S_AT glycine cleavage system protein H (aminomethyl carrier) hs
HG-U133A_2:213133_S_AT glycine cleavage system protein H (aminomethyl carrier) hs
HG-U133_PLUS_2:213133_S_AT glycine cleavage system protein H (aminomethyl carrier) hs
HG-U133A:213133_S_AT glycine cleavage system protein H (aminomethyl carrier) hs
HG-U133_PLUS_2:235613_AT IQ motif and WD repeats 1 hs
HG-U133B:235613_AT IQ motif and WD repeats 1 hs
HG-U133_PLUS_2:241209_AT IQ motif and WD repeats 1 hs
HG-U133B:241209_AT IQ motif and WD repeats 1 hs
HG-U133_PLUS_2:242717_AT IQ motif and WD repeats 1 hs
HG-U133B:242717_AT IQ motif and WD repeats 1 hs
HG-U95B:43790_R_AT IQ motif and WD repeats 1 hs
HG-U95C:62583_R_AT IQ motif and WD repeats 1 hs
HG-U95E:77373_AT IQ motif and WD repeats 1 hs
HG-U95D:87644_R_AT IQ motif and WD repeats 1 hs
HG-U95D:89417_AT IQ motif and WD repeats 1 hs
HU35KSUBA:RC_AA004274_AT IQ motif and WD repeats 1 hs
U133_X3P:HS.164064.0.A1_3P_AT IQ motif and WD repeats 1 hs
U133_X3P:HS.172249.0.A1_3P_AT IQ motif and WD repeats 1 hs
U133_X3P:HS.280168.0.A1_3P_AT IQ motif and WD repeats 1 hs
MOUSE430_2:1432164_A_AT glycine cleavage system protein H (aminomethyl carrier) mm
MOUSE430A_2:1432164_A_AT glycine cleavage system protein H (aminomethyl carrier) mm
MOE430A:1432164_A_AT glycine cleavage system protein H (aminomethyl carrier) mm
MU11KSUBA:AA422527_S_AT glycine cleavage system protein H (aminomethyl carrier) mm
MG-U74AV2:160385_AT glycine cleavage system protein H (aminomethyl carrier) mm
MU19KSUBC:TC40359_AT Glycine cleavage system protein H (aminomethyl carrier), mRNA (cDNA clone MGC:25958 IMAGE:4239181) mm
RAE230A:1367908_AT glycine cleavage system protein H (aminomethyl carrier) rn
RAT230_2:1367908_AT glycine cleavage system protein H (aminomethyl carrier) rn
RG-U34C:RC_AI103550_AT glycine cleavage system protein H (aminomethyl carrier) rn
RG-U34C:RC_AI172253_S_AT glycine cleavage system protein H (aminomethyl carrier) rn
YEAST_2:1770632_AT H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6546 glycine catabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5960 glycine cleavage complex inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA95820 Hypothetical protein F52A8.5 [Caenorhabditis elegans] ref|NP_492075.1| F52A8.5 [Caenorhabditis elegans] 2.0E-77
blast CAE66592 Hypothetical protein CBG11916 [Caenorhabditis briggsae] 6.0E-72
Protein Domains
Database ID Description E-Value
Pfam IPR002930 EMBL-EBI Glycine cleavage H-protein 1.8E-76
Sequence
Target Sequence 
>C. ELEGANS:173868_S_AT
gaaccaactttgtcgtgctgttccatctcttcgttccgctgtcagattggcctcatctgg
tcgtctatacacgaagaaacatgaatgggtgctcgtagagaacggtgttggaaccgttgg
aattacggactttgcaacggaacaactcggagatgtcgtcttcattgagctgccggatga
gggaactgaaatttcaaaaggagactcaactggagctgttgagagtgtgaaagccgcttc
tgacatttatgcaccaatctctggaactgtaacccagaaaaatgtgaaattggaggaaga
agccggtctgatcaacaagagtccattcgaaaagggatggttgtacaaattgaaagttaa
gtcagcagacgagctggaaaaacttcttaacgaggaacagtatgcaaagttcaagaagga
cgaagaagctgcccactaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACCAACTTTGTCGTGCTGTTCCA 516 345 16 Antisense
GCTGTCAGATTGGCCTCATCTGGTC 591 295 53 Antisense
CCTCATCTGGTCGTCTATACACGAA 114 269 66 Antisense
GGGTGCTCGTAGAGAACGGTGTTGG 387 495 102 Antisense
CGGACTTTGCAACGGAACAACTCGG 308 241 141 Antisense
GAGATGTCGTCTTCATTGAGCTGCC 583 395 165 Antisense
TGAGCTGCCGGATGAGGGAACTGAA 324 563 181 Antisense
TGAAAGCCGCTTCTGACATTTATGC 350 573 243 Antisense
GCACCAATCTCTGGAACTGTAACCC 294 315 266 Antisense
GAAGCCGGTCTGATCAACAAGAGTC 626 343 314 Antisense
GAAGGACGAAGAAGCTGCCCACTAA 475 333 430 Antisense
Sequence Source Affymetrix Proprietary Database