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[173864_at] N-heparan sulfate sulfotransferase

Description CEK040F1R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F08B4.6 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D34077 /WB_GENE_ID=F08B4.6 /WP=CE07047 /CHR=4 /FEA=Genomic Cluster /DEF=N-heparan sulfate sulfotransferase; C.elegans cDNA clone yk40f1 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 75      4grp_highest_8_8 > 43      4grp_high_8_8 > 40      6grp_highest_nonorm > 77      6grp_total > 76      4grp_total > 55      6grp_highest_sub > In      6grp_cgc > 57      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 664
1 2 3 4 range
A 1233.2
(3)
P 		783.9
(-1.7)
P 		857.5
(-0.9)
P 		658.7
(-3)
P 		575
B 1322.9
(3)
P 		796.7
(-3)
P 		917.6
(-1.6)
P 		940.8
(-1.4)
P 		526
C 1255
(3)
P 		1029.3
(-1.3)
P 		1054.5
(-0.9)
P 		943.2
(-3)
P 		312
avr 1270.4
(3) 		870
(-2.7) 		943.2
(-1.6) 		847.6
(-3) 		423

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1044
1 2 3 4 5 6 range
A 1352.3
(-0.2)
P 		1137.9
(-3)
P 		1222.5
(-1.9)
P 		1189.3
(-2.3)
P 		1603.9
(3)
P 		1547.3
(2.3)
P 		466
B 1167
(-3)
P 		1251.5
(-2.3)
P 		1606.5
(0.7)
P 		1390.7
(-1.1)
P 		1275.2
(-2.1)
P 		1876.3
(3)
P 		709
C 832.5
(-3)
P 		1287.6
(-0.3)
P 		1207.5
(-0.8)
P 		1541.9
(1.1)
P 		1007.6
(-2)
P 		1861.4
(3)
P 		1029
avr 1117.3
(-3) 		1225.7
(-2) 		1345.5
(-0.9) 		1374
(-0.6) 		1295.6
(-1.3) 		1761.7
(3) 		644
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 353
1 2 3 4 range
A 449
(3)
P 		170.2
(-1.7)
P 		224.2
(-0.8)
P 		96.4
(-3)
P 		353
B 421.9
(3)
P 		196.8
(-3)
P 		212.6
(-2.6)
P 		200.5
(-2.9)
P 		225
C 415.5
(3)
P 		295.9
(0.2)
P 		367.5
(1.9)
P 		159
(-3)
P 		257
avr 428.8
(3) 		221
(-1.5) 		268.1
(-0.5) 		152
(-3) 		277

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 450
1 2 3 4 5 6 range
A 249.3
(-3)
P 		404.7
(1)
P 		266.2
(-2.6)
P 		301.6
(-1.7)
P 		484
(3)
P 		376.7
(0.3)
P 		235
B 406.1
(-2.3)
P 		416.6
(-2.1)
P 		390.1
(-2.6)
P 		368.3
(-3)
P 		518.1
(-0.3)
P 		699.7
(3)
P 		331
C 308.6
(-3)
P 		392.9
(-1.4)
P 		307.3
(-3)
P 		310.1
(-2.9)
P 		326.1
(-2.6)
P 		623.1
(3)
P 		316
avr 321.3
(-3) 		404.7
(-1) 		321.2
(-3) 		326.7
(-2.9) 		442.7
(-0) 		566.5
(3) 		245
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173864_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24736
Sequence Type Exemplar sequence
Representative Public ID CEK040F1R NCBI
Target Description CEK040F1R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F08B4.6 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D34077 /WB_GENE_ID=F08B4.6 /WP=CE07047 /CHR=4 /FEA=Genomic Cluster /DEF=N-heparan sulfate sulfotransferase; C.elegans cDNA clone yk40f1 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069090(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069090 NCBI Caenorhabditis elegans Heparan SulphoTransferase family member (hst-1) (hst-1) mRNA, complete cds. 11/11 A 
F08B4.6 ENSEMBL cdna:known chromosome:CEL140:IV:8688255:8691495:-1 gene:F08B4.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8688292-8691434(-) 99.85 99.85
Public Domain and Genome References
Gene Title N-heparan sulfate sulfotransferase
Gene Symbol hst-1
Ensembl F08B4.6
Entrez Gene 177675 Entrez gene
SwissProt Q19197 EMBL-EBI
WormBase CE33047 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.19125.1.S1_AT similar to N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 cfa
CHICKEN:GGA.15685.1.S1_S_AT similar to N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 gga
CHICKEN:GGAAFFX.23733.1.S1_AT similar to N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 gga
HG-U133_PLUS_2:220429_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 hs
HG-U133A:220429_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 hs
HG-FOCUS:220429_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 hs
HG-U133A_2:220429_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 hs
HU35KSUBB:RC_H11065_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 hs
U133_X3P:G4758765_3P_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 hs
HG-U95B:43658_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 hs
HG-U95B:47712_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 hs
HU35KSUBB:RC_H17308_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 hs
MG-U74CV2:137704_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 mm
MG-U74CV2:167325_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 mm
MOE430A:1453777_A_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 mm
MOUSE430A_2:1453777_A_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 mm
MOUSE430_2:1453777_A_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 mm
MOE430B:1443277_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 mm
MOUSE430_2:1443277_AT N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
8146 sulfotransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB37730 Heparan sulphotransferase protein 1 [Caenorhabditis elegans] ref|NP_501491.3| Heparan SulphoTransferase family member (hst-1) [Caenorhabditis elegans] 0.0
blast BAB62394.1 N-deacetylase/N-sulfotransferase [Caenorhabditis elegans] 0.0
blast BAB61757.1 N-deacetylase/N-sulfotransferase [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000863 EMBL-EBI Sulfotransferase 3.9E-69
Sequence
Target Sequence 
>C. ELEGANS:173864_AT
gaattcgtgcataatttacactactttttcttgtactttgccataactgcttgctcattt
ttgtaacaaatgcctgtacctattgatctttcgcttgtttat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATTCGTGCATAATTTACACTACT 42 325 2460 Antisense
GCATAATTTACACTACTTTTTCTTG 413 303 2468 Antisense
CACTACTTTTTCTTGTACTTTGCCA 568 197 2478 Antisense
CTTTGCCATAACTGCTTGCTCATTT 390 223 2495 Antisense
GCCATAACTGCTTGCTCATTTTTGT 77 281 2499 Antisense
ACTGCTTGCTCATTTTTGTAACAAA 200 99 2505 Antisense
GTAACAAATGCCTGTACCTATTGAT 328 461 2522 Antisense
AAATGCCTGTACCTATTGATCTTTC 336 119 2527 Antisense
GCCTGTACCTATTGATCTTTCGCTT 339 283 2531 Antisense
GTACCTATTGATCTTTCGCTTGTTT 167 459 2535 Antisense
ACCTATTGATCTTTCGCTTGTTTAT 463 87 2537 Antisense
Sequence Source Affymetrix Proprietary Database