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[173851_at]

Description C51112 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C06E7.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C51112 /WB_GENE_ID=C06E7.3 /WP=CE03959 /CHR=4 /FEA=Genomic Cluster /DEF=S-adenosylmethionine synthetase; C.elegans cDNA clone yk491c6 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 69      4grp_highest_8_8 > 12      4grp_high_8_8 > 39      6grp_highest_nonorm > 87      6grp_total > 79      4grp_total > 40      6grp_highest_sub > Cu      6grp_high1_sub > Cu      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 566
1 2 3 4 range
A 824.4
(-3)
P 		1175.7
(2.2)
P 		1229.3
(3)
P 		1118.3
(1.4)
P 		405
B 842.6
(-3)
P 		925.7
(-1)
P 		1096.1
(3)
P 		1092.8
(2.9)
P 		254
C 722.8
(-3)
P 		915.9
(-1)
P 		1288.9
(3)
P 		972.1
(-0.4)
P 		566
avr 796.6
(-3) 		1005.8
(0.1) 		1204.8
(3) 		1061.1
(0.9) 		408

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 918
1 2 3 4 5 6 range
A 1157.5
(-0.3)
P 		1189.2
(-0.1)
P 		762.4
(-3)
P 		916.3
(-1.9)
P 		1343.6
(1)
P 		1634.3
(3)
P 		872
B 1001.1
(-0.9)
P 		1142.3
(0.1)
P 		726.8
(-3)
P 		1146.8
(0.2)
P 		1158.3
(0.3)
P 		1522.3
(3)
P 		796
C 716.2
(-3)
P 		1217.2
(1)
P 		991.2
(-0.8)
P 		944.8
(-1.2)
P 		1066.7
(-0.2)
P 		1476.7
(3)
P 		761
avr 958.3
(-1.9) 		1182.9
(-0) 		826.8
(-3) 		1002.6
(-1.5) 		1189.5
(0) 		1544.4
(3) 		718
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 286
1 2 3 4 range
A 300.2
(2.2)
P 		255.2
(0.5)
P 		321.4
(3)
P 		163.7
(-3)
P 		158
B 268.7
(3)
P 		228.6
(-3)
P 		254
(0.8)
P 		232.9
(-2.4)
P 		40
C 239.3
(-1.4)
P 		263.3
(-0.9)
P 		449.2
(3)
P 		163.9
(-3)
P 		285
avr 269.4
(0.2) 		249
(-0.6) 		341.5
(3) 		186.8
(-3) 		155

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 402
1 2 3 4 5 6 range
A 213.4
(-1.9)
P 		423
(3)
P 		166
(-3)
P 		232.4
(-1.4)
P 		405.4
(2.6)
P 		397.9
(2.4)
P 		257
B 348.4
(-0.4)
P 		380.2
(0.1)
P 		176.5
(-3)
P 		303.7
(-1)
P 		470.6
(1.5)
P 		567.7
(3)
P 		391
C 265.5
(-1.5)
P 		371.4
(0.6)
P 		252.3
(-1.8)
P 		190
(-3)
P 		345.2
(0.1)
P 		494.4
(3)
P 		304
avr 275.8
(-1.4) 		391.5
(1) 		198.3
(-3) 		242
(-2.1) 		407.1
(1.3) 		486.7
(3) 		288
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173851_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24925
Sequence Type Exemplar sequence
Representative Public ID C51112 NCBI
Target Description C51112 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C06E7.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C51112 /WB_GENE_ID=C06E7.3 /WP=CE03959 /CHR=4 /FEA=Genomic Cluster /DEF=S-adenosylmethionine synthetase; C.elegans cDNA clone yk491c6 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182286(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182286 NCBI Caenorhabditis elegans C06E7.3b (C06E7.3) mRNA, complete cds. 11/11 None
C06E7.3b.1 ENSEMBL cdna:known chromosome:CEL140:IV:5843967:5846354:-1 gene:C06E7.3 11/11 A 
C06E7.3b.2 ENSEMBL cdna:known chromosome:CEL140:IV:5844059:5846354:-1 gene:C06E7.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
C06E7.3b.3 8/11 Cross Hyb Matching Probes A 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177354 2 1 NM_182286 173851_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5844112-5846327(-) 100.0 100.0
Public Domain and Genome References
Ensembl C06E7.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO44916 Hypothetical protein C06E7.3b [Caenorhabditis elegans] ref|NP_872086.1| C06E7.3b [Caenorhabditis elegans] 0.0
blast AAA82279 Hypothetical protein C06E7.3a [Caenorhabditis elegans] ref|NP_500871.1| C06E7.3a [Caenorhabditis elegans] sp|P50306|METL_CAEEL Probable S-adenosylmethionine synthetase C06E7.3 (Methionine adenosyltransferase) (AdoMet synthetase) 0.0
blast AAA82280 Hypothetical protein C06E7.1a [Caenorhabditis elegans] ref|NP_500872.1| C06E7.1a [Caenorhabditis elegans] sp|P50305|METK_CAEEL Probable S-adenosylmethionine synthetase C06E7.1 (Methionine adenosyltransferase) (AdoMet synthetase) 1.0E-178
Protein Domains
Database ID Description E-Value
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.8E-27
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 9.9E-99
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 9.3E-95
Sequence
Target Sequence 
>C. ELEGANS:173851_AT
attgcccattaaaattctgagattattgtaataatcaacttgataaaagtgcttgtaagc
cttctagtaatcacatacatttattttggtttcattttcacttgttcccctaaattccat
taattctattcccctgagcctcatttagattcaaaatcagtagcccccac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTGCCCATTAAAATTCTGAGATTA 385 7 1217 Antisense
AAAAGTGCTTGTAAGCCTTCTAGTA 376 131 1261 Antisense
GCTTGTAAGCCTTCTAGTAATCACA 161 307 1267 Antisense
AAGCCTTCTAGTAATCACATACATT 467 149 1273 Antisense
ACATACATTTATTTTGGTTTCATTT 332 105 1289 Antisense
TATTTTGGTTTCATTTTCACTTGTT 584 661 1298 Antisense
TGTTCCCCTAAATTCCATTAATTCT 134 563 1319 Antisense
AATTCCATTAATTCTATTCCCCTGA 603 175 1329 Antisense
CTATTCCCCTGAGCCTCATTTAGAT 28 223 1342 Antisense
CCCCTGAGCCTCATTTAGATTCAAA 585 261 1347 Antisense
TAGATTCAAAATCAGTAGCCCCCAC 28 653 1362 Antisense
Sequence Source Affymetrix Proprietary Database