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[173843_s_at] protein kinase

Description AV197656 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T07A9.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV197656 /WB_GENE_ID=T07A9.3 /WP=CE17212 /CHR=4 /FEA=Genomic Cluster /DEF=protein kinase; Caenorhabditis elegans cDNA clone:yk669b10 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 06      4grp_highest_8_8 > 42      4grp_high_8_8 > 41      6grp_highest_nonorm > 40      6grp_total > 07      4grp_total > 55      6grp_highest_sub > De      6grp_cgc > 20      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 419
1 2 3 4 range
A 927.1
(3)
P 		641.6
(-1.4)
P 		602.8
(-2)
P 		536.6
(-3)
P 		391
B 899.6
(3)
P 		657.8
(-0.7)
P 		630.6
(-1.1)
P 		508.1
(-3)
P 		392
C 918.2
(3)
P 		633.6
(-2)
P 		774.4
(0.5)
P 		573.6
(-3)
P 		345
avr 915
(3) 		644.3
(-1.3) 		669.3
(-0.9) 		539.4
(-3) 		376

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 424
1 2 3 4 5 6 range
A 732.8
(2.9)
P 		736.6
(3)
P 		545
(-2.2)
P 		701.9
(2.1)
P 		537.4
(-2.4)
P 		514.7
(-3)
P 		222
B 887.8
(3)
P 		650
(-1.4)
P 		636.9
(-1.6)
P 		604
(-2.2)
P 		560.7
(-3)
P 		645.4
(-1.4)
P 		327
C 938.8
(3)
P 		693.7
(-0.6)
P 		818.8
(1.2)
P 		569.5
(-2.4)
P 		529
(-3)
P 		575.1
(-2.3)
P 		410
avr 853.1
(3) 		693.4
(-0.1) 		666.9
(-0.6) 		625.1
(-1.4) 		542.4
(-3) 		578.4
(-2.3) 		311
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 259
1 2 3 4 range
A 337.5
(3)
P 		139.3
(-1.6)
P 		157.6
(-1.2)
P 		78.5
(-3)
P 		259
B 286.9
(3)
P 		162.5
(-1.2)
P 		146.1
(-1.7)
P 		108.3
(-3)
P 		179
C 304
(3)
P 		182.2
(-0.5)
P 		269.9
(2)
P 		96.7
(-3)
P 		207
avr 309.5
(3) 		161.3
(-1.1) 		191.2
(-0.3) 		94.5
(-3) 		215

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 234
1 2 3 4 5 6 range
A 135.1
(-2.3)
P 		262
(3)
P 		118.7
(-3)
P 		178
(-0.5)
P 		162.2
(-1.2)
P 		125.3
(-2.7)
P 		143
B 308.9
(3)
P 		216.4
(-0.6)
P 		154.7
(-3)
P 		160
(-2.8)
P 		227.8
(-0.2)
P 		240.7
(0.3)
P 		154
C 348
(3)
P 		211.7
(-0.5)
P 		208.4
(-0.6)
P 		114.5
(-3)
P 		171.2
(-1.5)
P 		192.5
(-1)
P 		234
avr 264
(3) 		230
(1.2) 		160.6
(-2.5) 		150.8
(-3) 		187.1
(-1.1) 		186.2
(-1.1) 		113
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173843_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24953
Sequence Type Exemplar sequence
Representative Public ID AV197656 NCBI
Target Description AV197656 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T07A9.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV197656 /WB_GENE_ID=T07A9.3 /WP=CE17212 /CHR=4 /FEA=Genomic Cluster /DEF=protein kinase; Caenorhabditis elegans cDNA clone:yk669b10 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067521(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067521 NCBI Caenorhabditis elegans Kinase, GLH-Binding family member (kgb-1) (kgb-1) mRNA, complete cds. 11/11 None
SNAP00000017765 ENSEMBL cdna:SNAP chromosome:CEL140:IV:405948:408034:-1 11/11 None
GENEFINDER00000017771 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:405948:408886:-1 11/11 None
T07A9.3 ENSEMBL cdna:known chromosome:CEL140:IV:405683:410026:-1 gene:T07A9.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:405948-408898(-) 95.56 95.56
Public Domain and Genome References
Gene Title protein kinase
Gene Symbol kgb-1
Ensembl T07A9.3
Entrez Gene 176866 Entrez gene
SwissProt O44408 EMBL-EBI
WormBase CE29466 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from sequence similarity QuickGO AmiGO
5737 cytoplasm inferred from sequence similarity QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4707 MAP kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
17151 DEAD/H-box RNA helicase binding inferred from physical interaction QuickGO AmiGO
4705 JUN kinase activity inferred from direct assay QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK39276 Kinase, glh-binding protein 1 [Caenorhabditis elegans] ref|NP_499922.1| Kinase, GLH-Binding family member (kgb-1) [Caenorhabditis elegans] 0.0
blast T32577 hypothetical protein T07A9.3 - Caenorhabditis elegans 0.0
blast CAE67936 Hypothetical protein CBG13536 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-65
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 8.0E-72
hanks 3.1.1 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; CDC2Hs 6.0E-67
hanks 3.1.1 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; CDC2Hs 3.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.4E-72
Pfam IPR000719 EMBL-EBI Protein kinase 8.3E-69
Sequence
Target Sequence 
>C. ELEGANS:173843_S_AT
agactttggattggcccggaagaagaatgtggacacatcgatgagaatgtcagattatgt
ggtgacaagatactacagagcccccgaagttatattaggtcttccctattcggaaaaagt
agatatttggtcagtcggatgtatatttgctgaaatgatcaaccataccgtacttttccc
tggaaaagatcgaattgatcaatggactaaaatttatagtgttttgggaacaccagatga
tcatttcattagtcaattgggtcagtcagctgctatgtatgttcgatcgttgccacgtca
tcaggcacgagcattcagtgaaatcgttcctgataccaattttctgccggagactgaaaa
tccaagggtccatttgacagcacacgtggctcgtgatctcctgttcaatatgttgaaaat
caatcctgaagaaaggtattcagttgaagatgcactgaatcatccatatgttaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGACTTTGGATTGGCCCGGAAGAAG 376 1 622 Antisense
CTACAGAGCCCCCGAAGTTATATTA 650 225 694 Antisense
TATATTAGGTCTTCCCTATTCGGAA 40 661 712 Antisense
TGGGAACACCAGATGATCATTTCAT 394 553 846 Antisense
TAGTCAATTGGGTCAGTCAGCTGCT 20 653 871 Antisense
GTCAGCTGCTATGTATGTTCGATCG 676 465 886 Antisense
CCACGTCATCAGGCACGAGCATTCA 331 275 914 Antisense
CGAGCATTCAGTGAAATCGTTCCTG 675 243 929 Antisense
CGTTCCTGATACCAATTTTCTGCCG 102 247 946 Antisense
TGAAAATCCAAGGGTCCATTTGACA 562 571 976 Antisense
ATGCACTGAATCATCCATATGTTAA 226 43 1071 Antisense
Sequence Source Affymetrix Proprietary Database