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[173834_at] protein kinase

Description AV186138 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F28C10.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV186138 /WB_GENE_ID=F28C10.3 /WP=CE07156 /CHR=X /FEA=Genomic Cluster /DEF=protein kinase; Caenorhabditis elegans cDNA clone:yk495f3 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 82      6grp_high1_10_10 > 28      4grp_high_8_8 > 34      6grp_highest_nonorm > 90      6grp_total > 28      4grp_total > 45      6grp_highest_sub > Cu      6grp_high1_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 117.1
(0.7)
P 		153.5
(3)
P 		96.8
(-0.6)
P 		58
(-3)
A 		96
B 153.8
(3)
P 		128.7
(1.1)
P 		74.2
(-3)
A 		92.2
(-1.6)
P 		80
C 138.2
(3)
P 		82.2
(-3)
P 		98.1
(-1.3)
P 		97.7
(-1.3)
P 		56
avr 136.4
(3) 		121.5
(1.3) 		89.7
(-2.2) 		82.6
(-3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 150
1 2 3 4 5 6 range
A 219.4
(3)
P 		106.7
(-1.5)
P 		80.7
(-2.6)
P 		70.5
(-3)
P 		113.3
(-1.3)
P 		131.8
(-0.5)
P 		149
B 211.2
(3)
P 		128.6
(-0.9)
P 		83.6
(-3)
P 		103.7
(-2.1)
P 		114
(-1.6)
P 		136.6
(-0.5)
P 		128
C 209.3
(3)
P 		123.3
(-0.7)
P 		88.4
(-2.2)
P 		69.9
(-3)
P 		102
(-1.6)
P 		118.5
(-0.9)
P 		139
avr 213.3
(3) 		119.5
(-1.3) 		84.2
(-2.9) 		81.4
(-3) 		109.8
(-1.7) 		129
(-0.8) 		132
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 42.6
(3)
P 		33.3
(1.4)
P 		25.3
(-0)
P 		8.5
(-3)
A 		34
B 49
(3)
P 		31.8
(-0.2)
P 		17.2
(-3)
A 		19.6
(-2.5)
P 		32
C 45.8
(3)
P 		23.6
(-1.5)
P 		34.2
(0.6)
P 		16.5
(-3)
P 		29
avr 45.8
(3) 		29.6
(-0.1) 		25.6
(-0.9) 		14.9
(-3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 40.5
(3)
P 		38
(2.3)
P 		17.6
(-3)
P 		17.9
(-2.9)
P 		34.2
(1.3)
P 		32.1
(0.8)
P 		23
B 73.5
(3)
P 		42.8
(-0.5)
P 		20.3
(-3)
P 		27.5
(-2.2)
P 		46.3
(-0.1)
P 		50.9
(0.5)
P 		53
C 77.6
(3)
P 		37.6
(-0.8)
P 		22.5
(-2.2)
P 		14.1
(-3)
P 		33
(-1.2)
P 		39.7
(-0.6)
P 		64
avr 63.9
(3) 		39.5
(-0.3) 		20.1
(-3) 		19.8
(-3) 		37.8
(-0.6) 		40.9
(-0.1) 		44
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173834_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.26197
Sequence Type Exemplar sequence
Representative Public ID AV186138 NCBI
Target Description AV186138 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F28C10.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV186138 /WB_GENE_ID=F28C10.3 /WP=CE07156 /CHR=X /FEA=Genomic Cluster /DEF=protein kinase; Caenorhabditis elegans cDNA clone:yk495f3 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075713(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075713 NCBI Caenorhabditis elegans F28C10.3 (F28C10.3) mRNA, complete cds. 10/11 None
F28C10.3 ENSEMBL cdna:known chromosome:CEL140:X:531886:535750:-1 gene:F28C10.3 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:531896-535749(-) 94.14 100.0
Public Domain and Genome References
Gene Title protein kinase
Ensembl F28C10.3
Entrez Gene 180405 Entrez gene
SwissProt Q19858 EMBL-EBI
WormBase CE07156 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB00607 Hypothetical protein F28C10.3 [Caenorhabditis elegans] ref|NP_508114.1| F28C10.3 [Caenorhabditis elegans] 0.0
blast CAE63652 Hypothetical protein CBG08151 [Caenorhabditis briggsae] 5.0E-93
blast XP_699952 PREDICTED: similar to ribosomal protein S6 kinase, 90kDa, polypeptide 2 isoform a [Danio rerio] 5.0E-49
Protein Families
Method ID Description E-Value
hanks 1.4.5 AGC group; AGC IV GPCR kinases; IT11 3.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.0E-53
Sequence
Target Sequence 
>C. ELEGANS:173834_AT
atgcatttttatacgacaccctgtcccactttactggactgatattgacttcacggaccc
atccctgtgcaacaactagtcatccaacaagaatggatttcaatatcattttctattgcc
atgccaagtattcactggactttttgaccaataggttccccttttgccacttctcccgtt
tattgaaccctattttcccgattccaacgttttgaaaacataaacctctacgttcattct
aatttttcaatttaaaatgttaccctttttctaggattttcaattgaaactcttatgggc
cctttttccgttttgtcattggttttttatcagcatttcagccgacttttaaagtcattc
actttcccgtttacatgtactttcatgggttcacccct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCATTTTTATACGACACCCTGTC 473 43 978 Antisense
AACATAAACCTCTACGTTCATTCTA 264 137 1194 Antisense
CTCTACGTTCATTCTAATTTTTCAA 63 231 1203 Antisense
TCAATTGAAACTCTTATGGGCCCTT 628 631 1257 Antisense
TTTGTCATTGGTTTTTTATCAGCAT 533 667 1289 Antisense
TTATCAGCATTTCAGCCGACTTTTA 635 679 1304 Antisense
CAGCATTTCAGCCGACTTTTAAAGT 194 205 1308 Antisense
AGCCGACTTTTAAAGTCATTCACTT 243 83 1317 Antisense
TCATTCACTTTCCCGTTTACATGTA 597 619 1332 Antisense
TCACTTTCCCGTTTACATGTACTTT 249 625 1336 Antisense
CATGTACTTTCATGGGTTCACCCCT 451 209 1351 Antisense
Sequence Source Affymetrix Proprietary Database