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[173829_at] Kinesin heavy chain

Description CEK010G3F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=R05D3.7 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D34677 /WB_GENE_ID=R05D3.7 /WP=CE26945 /CHR=3 /FEA=Genomic Cluster /GEN=unc-116 /DEF=Kinesin heavy chain; C.elegans cDNA clone yk10g3 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 07      4grp_highest_8_8 > 34      4grp_high_8_8 > 57      6grp_highest_nonorm > 14      6grp_total > 06      4grp_total > 62      6grp_highest_sub > De      6grp_cgc > 13      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3026
1 2 3 4 range
A 4483
(3)
P 		3047.3
(-0.7)
P 		3809.3
(1.3)
P 		2125.3
(-3)
P 		2358
B 5151.3
(3)
P 		3469.5
(-2.4)
P 		4109.5
(-0.3)
P 		3281.1
(-3)
P 		1870
C 4311.7
(3)
P 		3474.5
(0.1)
P 		3907.3
(1.6)
P 		2572
(-3)
P 		1740
avr 4648.7
(3) 		3330.4
(-1) 		3942
(0.9) 		2659.5
(-3) 		1989

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2680
1 2 3 4 5 6 range
A 5565.1
(3)
P 		4269
(0.1)
P 		3180.4
(-2.3)
P 		2885.4
(-3)
P 		3933.4
(-0.7)
P 		3215.4
(-2.3)
P 		2680
B 4120.5
(1.5)
P 		3513.2
(-0.8)
P 		3203.5
(-2)
P 		4525.4
(3)
P 		3096.5
(-2.4)
P 		2928.6
(-3)
P 		1597
C 4320.2
(3)
P 		4122.6
(2.1)
P 		3573.7
(-0.3)
P 		3395.8
(-1)
P 		2945.6
(-3)
P 		3195.6
(-1.9)
P 		1375
avr 4668.6
(3) 		3968.3
(0.3) 		3319.2
(-2.2) 		3602.2
(-1.1) 		3325.2
(-2.2) 		3113.2
(-3) 		1555
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1332
1 2 3 4 range
A 1632.2
(3)
P 		661.5
(-1.4)
P 		995.8
(0.1)
P 		311.1
(-3)
P 		1321
B 1642.7
(3)
P 		856.9
(-2)
P 		952.3
(-1.4)
P 		699.2
(-3)
P 		944
C 1427.3
(3)
P 		998.9
(0.4)
P 		1361.8
(2.6)
P 		433.7
(-3)
P 		994
avr 1567.4
(3) 		839.1
(-1) 		1103.3
(0.4) 		481.3
(-3) 		1086

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 918
1 2 3 4 5 6 range
A 1025.9
(-0.6)
P 		1518.3
(3)
P 		692.6
(-3)
P 		731.8
(-2.7)
P 		1186.9
(0.6)
P 		782.9
(-2.3)
P 		826
B 1433.7
(3)
P 		1169.4
(0.6)
P 		777.9
(-3)
P 		1198.6
(0.8)
P 		1258.1
(1.4)
P 		1092.1
(-0.1)
P 		656
C 1601.3
(3)
P 		1257.9
(0.8)
P 		909.6
(-1.5)
P 		682.9
(-3)
P 		953.2
(-1.2)
P 		1069.8
(-0.5)
P 		918
avr 1353.6
(3) 		1315.2
(2.6) 		793.4
(-3) 		871.1
(-2.2) 		1132.7
(0.6) 		981.6
(-1) 		560
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173829_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23886
Sequence Type Exemplar sequence
Representative Public ID CEK010G3F NCBI
Target Description CEK010G3F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=R05D3.7 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D34677 /WB_GENE_ID=R05D3.7 /WP=CE26945 /CHR=3 /FEA=Genomic Cluster /GEN=unc-116 /DEF=Kinesin heavy chain; C.elegans cDNA clone yk10g3 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066441(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066441 NCBI Caenorhabditis elegans UNCoordinated family member (unc-116) (unc-116) mRNA, complete cds. 10/11 A 
R05D3.7 ENSEMBL cdna:known chromosome:CEL140:III:8351164:8355089:-1 gene:R05D3.7 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8351108-8355007(-) 99.48 100.0
Public Domain and Genome References
Gene Title Kinesin heavy chain
Gene Symbol unc-116
Ensembl R05D3.7
Entrez Gene 176179 Entrez gene
SwissProt P34540 EMBL-EBI
WormBase CE26945 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:17350_AT kinesin motor protein-related at
ATH1-121501:263762_AT kinesin motor protein-related at
CANINE:1597685_AT similar to Kinesin heavy chain (Ubiquitous kinesin heavy chain) (UKHC) cfa
CANINE_2:CFA.20889.1.S1_AT similar to Kinesin heavy chain (Ubiquitous kinesin heavy chain) (UKHC) cfa
CANINE_2:CFA.21106.1.S1_S_AT similar to Kinesin heavy chain (Ubiquitous kinesin heavy chain) (UKHC) cfa
CANINE_2:CFA.922.1.A1_AT similar to Kinesin heavy chain (Ubiquitous kinesin heavy chain) (UKHC) cfa
CANINE_2:CFAAFFX.7041.1.S1_S_AT similar to Kinesin heavy chain (Ubiquitous kinesin heavy chain) (UKHC) cfa
DROSOPHILA_2:1641312_AT Kinesin heavy chain dm
DROSGENOME1:141504_AT Kinesin heavy chain dm
CHICKEN:GGA.1342.1.S1_AT kinesin family member 5B gga
CHICKEN:GGA.9857.1.S1_S_AT kinesin family member 5B gga
CHICKEN:GGAAFFX.4520.1.S1_S_AT kinesin family member 5B gga
HG-FOCUS:201991_S_AT kinesin family member 5B hs
HG-U133A:201991_S_AT kinesin family member 5B hs
HG-U133A_2:201991_S_AT kinesin family member 5B hs
HG-U133_PLUS_2:201991_S_AT kinesin family member 5B hs
HG-U133A:201992_S_AT kinesin family member 5B hs
HG-U133A_2:201992_S_AT kinesin family member 5B hs
HG-U133_PLUS_2:201992_S_AT kinesin family member 5B hs
HU35KSUBD:RC_H29042_S_AT kinesin family member 5B hs
HUGENEFL:X65873_AT kinesin family member 5B hs
U133_X3P:G4758647_3P_A_AT kinesin family member 5B hs
U133_X3P:HS.149436.1.A1_3P_AT kinesin family member 5B hs
U133_X3P:HS.149436.0.A1_3P_A_AT kinesin family member 5B hs
HG-U133_PLUS_2:224662_AT kinesin family member 5B hs
HG-U133B:224662_AT kinesin family member 5B hs
HG-U95AV2:34732_AT kinesin family member 5B hs
HG-U95B:57037_AT Kinesin family member 5B hs
HG-U95C:62885_AT Kinesin family member 5B hs
HG-U95D:85403_R_AT Kinesin family member 5B hs
HU35KSUBA:RC_AA416546_AT Kinesin family member 5B hs
MOE430A:1418428_AT kinesin family member 5B mm
MOUSE430_2:1418428_AT kinesin family member 5B mm
MOUSE430A_2:1418428_AT kinesin family member 5B mm
MOUSE430A_2:1418429_AT kinesin family member 5B mm
MOUSE430_2:1418429_AT kinesin family member 5B mm
MOE430A:1418429_AT kinesin family member 5B mm
MOUSE430_2:1418431_AT kinesin family member 5B mm
MOUSE430A_2:1418431_AT kinesin family member 5B mm
MOE430A:1418431_AT kinesin family member 5B mm
MOUSE430_2:1418427_AT kinesin family member 5B mm
MOUSE430A_2:1418427_AT kinesin family member 5B mm
MOE430A:1418427_AT kinesin family member 5B mm
MOUSE430A_2:1418430_AT kinesin family member 5B mm
MOE430A:1418430_AT kinesin family member 5B mm
MOUSE430_2:1418430_AT kinesin family member 5B mm
MU11KSUBA:U86090_S_AT kinesin family member 5B mm
MU11KSUBB:MSA.40717.0_S_AT kinesin family member 5B mm
MU11KSUBB:MSA.30643.0_S_AT kinesin family member 5B mm
MU11KSUBB:MSA.29755.0_S_AT kinesin family member 5B mm
MU11KSUBB:MSA.28565.0_S_AT kinesin family member 5B mm
MG-U74BV2:113589_R_AT kinesin family member 5B mm
MG-U74BV2:113588_F_AT kinesin family member 5B mm
MG-U74AV2:160417_AT kinesin family member 5B mm
MG-U74CV2:168342_R_AT Kinesin family member 5B (Kif5b), mRNA mm
MU19KSUBB:TC30353_AT Kinesin family member 5B (Kif5b), mRNA mm
MU19KSUBC:TC39604_AT Kinesin family member 5B (Kif5b), mRNA mm
RG-U34B:RC_AA924572_AT kinesin family member 5B rn
RAE230A:1372444_AT kinesin family member 5B rn
RAT230_2:1372444_AT kinesin family member 5B rn
RAE230B:1378096_AT kinesin family member 5B rn
RAT230_2:1378096_AT kinesin family member 5B rn
RG-U34B:RC_AI043798_AT Kinesin family member 5B rn
RG-U34C:RC_AI102819_AT Kinesin family member 5B rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5875 microtubule associated complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3774 motor activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK21446 Uncoordinated protein 116 [Caenorhabditis elegans] ref|NP_498842.1| UNCoordinated family member (unc-116) [Caenorhabditis elegans] sp|P34540|KINH_CAEEL Kinesin heavy chain dbj|BAA32594.1| kinesin Heavy chain [Caenorhabditis elegans] gb|AAA28155.1| kinesin heavy chain 0.0
blast CAE71280 Hypothetical protein CBG18164 [Caenorhabditis briggsae] 0.0
blast XP_877731 PREDICTED: similar to Kinesin heavy chain (Ubiquitous kinesin heavy chain) (UKHC) isoform 6 [Bos taurus] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001752 EMBL-EBI Kinesin, motor region 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:173829_AT
ttccgcctttttgttctaatagtctgtttctatacgattttcctgtttcagttttactaa
tctgacacgaggttttgtctggttcttccccccgtcacccaccaacactcctatgattgt
tttttgcatgcgtttgagtgtctttaaagcttgcttgctaaatccccctatcattcttca
taagaaatcaacttgtttcgtttctgcacaattcggcccccaaatccccgcacatcccaa
ttgcgtaatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCCGCCTTTTTGTTCTAATAGTCT 233 697 2776 Antisense
AGTCTGTTTCTATACGATTTTCCTG 141 59 2796 Antisense
CTATACGATTTTCCTGTTTCAGTTT 50 225 2805 Antisense
AGTTTTACTAATCTGACACGAGGTT 584 1 2825 Antisense
GACACGAGGTTTTGTCTGGTTCTTC 225 365 2839 Antisense
ACCCACCAACACTCCTATGATTGTT 135 89 2872 Antisense
TTTGCATGCGTTTGAGTGTCTTTAA 386 667 2898 Antisense
TAAAGCTTGCTTGCTAAATCCCCCT 651 639 2920 Antisense
ATCCCCCTATCATTCTTCATAAGAA 48 39 2937 Antisense
AACTTGTTTCGTTTCTGCACAATTC 592 137 2965 Antisense
CCCCGCACATCCCAATTGCGTAATT 470 261 3001 Antisense
Sequence Source Affymetrix Proprietary Database