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[173825_at] f-spondin like protein

Description CEK026F7R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T21B6.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D33135 /WB_GENE_ID=T21B6.3 /WP=CE03681 /CHR=X /FEA=Genomic Cluster /DEF=f-spondin like protein; C.elegans cDNA clone yk26f7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 15      4grp_highest_8_8 > 40      4grp_high_8_8 > 50      6grp_highest_nonorm > 02      6grp_total > 15      4grp_total > 53      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5242
1 2 3 4 range
A 5360.9
(3)
P 		4257.1
(1.7)
P 		3109.3
(0.4)
P 		118.5
(-3)
P 		5242
B 5289.1
(3)
P 		4106.7
(1.6)
P 		2867.5
(0.2)
P 		133.2
(-3)
P 		5156
C 4827.4
(3)
P 		4263
(2.3)
P 		3348.9
(1.1)
P 		163.2
(-3)
P 		4664
avr 5159.1
(3) 		4208.9
(1.9) 		3108.6
(0.5) 		138.3
(-3) 		5021

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4804
1 2 3 4 5 6 range
A 4212.8
(3)
P 		3831.3
(2.4)
P 		1924.7
(-0.5)
P 		865.6
(-2.1)
P 		875.5
(-2.1)
P 		298.7
(-3)
P 		3914
B 5102.5
(3)
P 		4197.7
(1.8)
P 		1935.9
(-1.2)
P 		1117.2
(-2.2)
P 		851.8
(-2.6)
P 		530.4
(-3)
P 		4572
C 5051
(3)
P 		3884.4
(1.5)
P 		2059.6
(-0.8)
P 		885.3
(-2.3)
P 		832.1
(-2.4)
P 		354.3
(-3)
P 		4697
avr 4788.8
(3) 		3971.1
(1.9) 		1973.4
(-0.8) 		956
(-2.2) 		853.1
(-2.4) 		394.5
(-3) 		4394
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1935
1 2 3 4 range
A 1951.9
(3)
P 		924.1
(-0.2)
P 		812.8
(-0.5)
P 		17.4
(-3)
P 		1935
B 1686.6
(3)
P 		1014.2
(0.6)
P 		664.5
(-0.7)
P 		28.4
(-3)
P 		1658
C 1598
(3)
P 		1225.6
(1.6)
P 		1167.2
(1.4)
P 		27.5
(-3)
P 		1571
avr 1745.5
(3) 		1054.6
(0.6) 		881.5
(-0) 		24.4
(-3) 		1721

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1800
1 2 3 4 5 6 range
A 776.6
(0.3)
P 		1362.6
(3)
P 		419.2
(-1.4)
P 		219.5
(-2.3)
P 		264.2
(-2.1)
P 		72.7
(-3)
P 		1290
B 1775.4
(3)
P 		1397.2
(1.6)
P 		470.1
(-2)
P 		295.9
(-2.6)
P 		346.1
(-2.4)
P 		197.8
(-3)
P 		1578
C 1872.2
(3)
P 		1185.3
(0.6)
P 		524.2
(-1.6)
P 		178
(-2.8)
P 		269.2
(-2.5)
P 		118.6
(-3)
P 		1754
avr 1474.7
(3) 		1315
(2.3) 		471.2
(-1.5) 		231.1
(-2.5) 		293.2
(-2.3) 		129.7
(-3) 		1345
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173825_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25996
Sequence Type Exemplar sequence
Representative Public ID CEK026F7R NCBI
Target Description CEK026F7R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T21B6.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D33135 /WB_GENE_ID=T21B6.3 /WP=CE03681 /CHR=X /FEA=Genomic Cluster /DEF=f-spondin like protein; C.elegans cDNA clone yk26f7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077428(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077428 NCBI Caenorhabditis elegans T21B6.3 (T21B6.3) mRNA, complete cds. 11/11 None
T21B6.3 ENSEMBL cdna:known chromosome:CEL140:X:10945076:10948892:-1 gene:T21B6.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:10945101-10948825(-) 91.0 100.0
Public Domain and Genome References
Gene Title f-spondin like protein
Ensembl T21B6.3
Entrez Gene 181284 Entrez gene
SwissProt Q22631 EMBL-EBI
WormBase CE31621 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92014 Hypothetical protein T21B6.3 [Caenorhabditis elegans] ref|NP_509829.2| T21B6.3 [Caenorhabditis elegans] 0.0
blast CAE70791 Hypothetical protein CBG17547 [Caenorhabditis briggsae] 0.0
blast NP_509195 C36B7.5b [Caenorhabditis elegans] gb|AAK68232.1| Hypothetical protein C36B7.5b [Caenorhabditis elegans] 2.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 1.1E-11
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 8.9E-12
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 8.4E-14
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 8.6E-15
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 2.2E-7
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 1.1E-4
Pfam IPR003014 EMBL-EBI N/apple PAN 2.4E-10
Sequence
Target Sequence 
>C. ELEGANS:173825_AT
aatcagtcagacagggttacatcgcccatttctttcatatttatgtttttcaatttgtac
ttatattcaatactttcatacaacaccgcctcaaatagtcgaatctctatatctagtcaa
ttaattgtatttttcacgtcttccattatgcaaatgcctttcaacgataggttaaattaa
agtttgttttatctttgaaatttatgttttaatagcattcactgccataatttgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCAGTCAGACAGGGTTACATCGC 646 169 2398 Antisense
ATCGCCCATTTCTTTCATATTTATG 15 39 2418 Antisense
TGTTTTTCAATTTGTACTTATATTC 598 561 2441 Antisense
TATTCAATACTTTCATACAACACCG 345 663 2461 Antisense
CATACAACACCGCCTCAAATAGTCG 18 213 2474 Antisense
ACACCGCCTCAAATAGTCGAATCTC 27 111 2480 Antisense
GTCGAATCTCTATATCTAGTCAATT 640 473 2495 Antisense
TTAATTGTATTTTTCACGTCTTCCA 308 685 2518 Antisense
CACGTCTTCCATTATGCAAATGCCT 477 199 2532 Antisense
GCAAATGCCTTTCAACGATAGGTTA 118 317 2547 Antisense
AATAGCATTCACTGCCATAATTTGC 103 173 2608 Antisense
Sequence Source Affymetrix Proprietary Database