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[173813_at] glutamate-cysteine ligase

Description CEC8844 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F37B12.2 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C11884 /WB_GENE_ID=F37B12.2 /WP=CE03290 /CHR=2 /FEA=Genomic Cluster /DEF=glutamate-cysteine ligase; C.elegans cDNA clone yk135c5 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 63      6grp_high1_10_10 > 36      4grp_highest_8_8 > 35      4grp_high_8_8 > 56      6grp_highest_nonorm > 73      6grp_total > 36      4grp_total > 63      6grp_highest_sub > Cu      6grp_cgc > 16      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 561
1 2 3 4 range
A 695.7
(3)
P 		363.9
(-2.6)
P 		568.7
(0.9)
P 		337.5
(-3)
P 		358
B 648.8
(3)
P 		501.8
(-3)
P 		582.4
(0.3)
P 		520.8
(-2.2)
P 		147
C 898
(3)
P 		483.1
(-2.5)
P 		698.6
(0.3)
P 		448.9
(-3)
P 		449
avr 747.5
(3) 		449.6
(-2.7) 		616.6
(0.5) 		435.7
(-3) 		312

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 297
1 2 3 4 5 6 range
A 448
(-2.5)
P 		591.8
(3)
P 		435.4
(-3)
P 		506.7
(-0.3)
P 		525
(0.4)
P 		481.9
(-1.2)
P 		156
B 668.4
(3)
P 		652.1
(2.6)
P 		633.2
(2)
P 		464.7
(-2.5)
P 		447.7
(-3)
P 		585.4
(0.7)
P 		221
C 732.6
(3)
P 		578.7
(-0.8)
P 		487
(-3)
P 		512.4
(-2.4)
P 		527.2
(-2)
P 		673.5
(1.6)
P 		246
avr 616.3
(3) 		607.5
(2.6) 		518.5
(-1.8) 		494.6
(-3) 		500
(-2.7) 		580.3
(1.2) 		122
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 248
1 2 3 4 range
A 253.3
(3)
P 		79
(-2.1)
P 		148.7
(-0.1)
P 		49.4
(-3)
P 		204
B 206.9
(3)
P 		123.9
(-2.2)
P 		135
(-1.5)
P 		111
(-3)
P 		96
C 297.3
(3)
P 		138.9
(-1.3)
P 		243.5
(1.5)
P 		75.7
(-3)
P 		222
avr 252.5
(3) 		113.9
(-1.8) 		175.7
(0.3) 		78.7
(-3) 		174

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 189
1 2 3 4 5 6 range
A 82.6
(-3)
P 		210.5
(3)
P 		94.8
(-2.4)
P 		128.5
(-0.8)
P 		158.4
(0.6)
P 		117.3
(-1.4)
P 		128
B 232.6
(3)
P 		217
(2.1)
P 		153.8
(-1.3)
P 		123.1
(-3)
P 		181.9
(0.2)
P 		218.3
(2.2)
P 		110
C 271.5
(3)
P 		176.6
(-0.4)
P 		124
(-2.3)
P 		103
(-3)
P 		170.6
(-0.6)
P 		225.5
(1.4)
P 		169
avr 195.6
(2.6) 		201.4
(3) 		124.2
(-2.6) 		118.2
(-3) 		170.3
(0.8) 		187
(2) 		83
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173813_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23489
Sequence Type Exemplar sequence
Representative Public ID CEC8844 NCBI
Target Description CEC8844 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F37B12.2 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C11884 /WB_GENE_ID=F37B12.2 /WP=CE03290 /CHR=2 /FEA=Genomic Cluster /DEF=glutamate-cysteine ligase; C.elegans cDNA clone yk135c5 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063526(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063526 NCBI Caenorhabditis elegans gamma GlutamylCysteine Synthetase family member (gcs-1) (gcs-1) mRNA, complete cds. 11/11 None
F37B12.2.2 ENSEMBL cdna:known chromosome:CEL140:II:9035485:9038212:-1 gene:F37B12.2 11/11 None
F37B12.2.1 ENSEMBL cdna:known chromosome:CEL140:II:9035485:9038211:-1 gene:F37B12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9035536-9038208(-) 100.0 100.0
Public Domain and Genome References
Gene Title glutamate-cysteine ligase
Gene Symbol gcs-1
Ensembl F37B12.2
Entrez Gene 174438 Entrez gene
SwissProt Q20117 EMBL-EBI
WormBase CE32886 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.4308.1.S1_AT similar to Glutamate--cysteine ligase catalytic subunit (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS heavy chain) cfa
CANINE_2:CFAAFFX.4309.1.S1_S_AT similar to Glutamate--cysteine ligase catalytic subunit (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS heavy chain) cfa
DROSOPHILA_2:1625362_AT Glutamate-cysteine ligase catalytic subunit dm
DROSGENOME1:144677_AT Glutamate-cysteine ligase catalytic subunit dm
CHICKEN:GGA.10006.1.S1_AT similar to Glutamate--cysteine ligase catalytic subunit (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS heavy chain) gga
CHICKEN:GGAAFFX.24934.1.S1_AT similar to Glutamate--cysteine ligase catalytic subunit (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS heavy chain) gga
CHICKEN:GGAAFFX.24934.1.S1_S_AT similar to Glutamate--cysteine ligase catalytic subunit (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS heavy chain) gga
HG-U95AV2:31850_AT glutamate-cysteine ligase, catalytic subunit hs
HG-U133_PLUS_2:202923_S_AT glutamate-cysteine ligase, catalytic subunit hs
HG-U133A:202923_S_AT glutamate-cysteine ligase, catalytic subunit hs
HG-U133A_2:202923_S_AT glutamate-cysteine ligase, catalytic subunit hs
HU35KSUBA:RC_AA449306_AT glutamate-cysteine ligase, catalytic subunit hs
HU35KSUBC:RC_H93053_S_AT glutamate-cysteine ligase, catalytic subunit hs
HU35KSUBC:RC_H93482_AT glutamate-cysteine ligase, catalytic subunit hs
HUGENEFL:M90656_AT glutamate-cysteine ligase, catalytic subunit hs
U133_X3P:G4557624_3P_A_AT glutamate-cysteine ligase, catalytic subunit hs
U133_X3P:HS2.151393.2.S1_3P_S_AT glutamate-cysteine ligase, catalytic subunit hs
U133_X3P:HS.151393.0.S2_3P_AT glutamate-cysteine ligase, catalytic subunit hs
HG-U133_PLUS_2:1555330_AT glutamate-cysteine ligase, catalytic subunit hs
HG-U133A_2:202922_AT glutamate-cysteine ligase, catalytic subunit hs
HG-FOCUS:202922_AT glutamate-cysteine ligase, catalytic subunit hs
HG-U133_PLUS_2:202922_AT glutamate-cysteine ligase, catalytic subunit hs
HG-U133A:202922_AT glutamate-cysteine ligase, catalytic subunit hs
HG-U95B:49163_AT glutamate-cysteine ligase, catalytic subunit hs
HG-U95C:51519_AT glutamate-cysteine ligase, catalytic subunit hs
HG-U95C:52591_S_AT glutamate-cysteine ligase, catalytic subunit hs
HG-U95C:52592_R_AT glutamate-cysteine ligase, catalytic subunit hs
HG-U95C:56626_R_AT glutamate-cysteine ligase, catalytic subunit hs
HG-U95D:86240_AT Glutamate-cysteine ligase, catalytic subunit hs
MU11KSUBA:U85498_S_AT glutamate-cysteine ligase, catalytic subunit mm
MG-U74AV2:99649_AT glutamate-cysteine ligase, catalytic subunit mm
MOE430A:1424296_AT glutamate-cysteine ligase, catalytic subunit mm
MOUSE430A_2:1424296_AT glutamate-cysteine ligase, catalytic subunit mm
MOUSE430_2:1424296_AT glutamate-cysteine ligase, catalytic subunit mm
MOE430A:1455959_S_AT glutamate-cysteine ligase, catalytic subunit mm
MOUSE430A_2:1455959_S_AT glutamate-cysteine ligase, catalytic subunit mm
MOUSE430_2:1455959_S_AT glutamate-cysteine ligase, catalytic subunit mm
MG-U74BV2:111650_AT Glutamate-cysteine ligase, catalytic subunit, mRNA (cDNA clone MGC:30487 IMAGE:4195425) mm
MU11KSUBA:AA408634_RC_AT Glutamate-cysteine ligase, catalytic subunit, mRNA (cDNA clone MGC:30487 IMAGE:4195425) mm
MU19KSUBC:TC36493_AT Glutamate-cysteine ligase, catalytic subunit, mRNA (cDNA clone MGC:30487 IMAGE:4195425) mm
MU19KSUBC:TC36493_G_AT Glutamate-cysteine ligase, catalytic subunit, mRNA (cDNA clone MGC:30487 IMAGE:4195425) mm
RAT230_2:1370688_AT glutamate-cysteine ligase, catalytic subunit rn
RAE230A:1370688_AT glutamate-cysteine ligase, catalytic subunit rn
RT-U34:J05181_AT glutamate-cysteine ligase, catalytic subunit rn
RG-U34A:J05181_AT glutamate-cysteine ligase, catalytic subunit rn
RG-U34C:RC_AI232266_AT glutamate-cysteine ligase, catalytic subunit rn
RAE230A:1372523_AT glutamate-cysteine ligase, catalytic subunit rn
RAT230_2:1372523_AT glutamate-cysteine ligase, catalytic subunit rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6750 glutathione biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4357 glutamate-cysteine ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA90955 Hypothetical protein F37B12.2 [Caenorhabditis elegans] ref|NP_495927.2| gamma GlutamylCysteine Synthetase family member (gcs-1) [Caenorhabditis elegans] sp|Q20117|GSH1_CAEEL Glutamate--cysteine ligase (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS) 0.0
blast CAE59789 Hypothetical protein CBG03249 [Caenorhabditis briggsae] 0.0
blast AAB96970 gamma-glutamylcysteine synthetase [Onchocerca volvulus] sp|Q9NFN6|GSH1_ONCVO Glutamate--cysteine ligase (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004308 EMBL-EBI Glutamate-cysteine ligase catalytic subunit 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:173813_AT
aaacgtgcacattaggcgataattgattgattatgtgattttaatttatttatgttctat
acgtcgtgtttcccattccttctaggccttccatgattcacaatttttcgatgccatatc
aatttagttggccatctacattaaattactgatatgttgatgctattttctagtaagcag
atgtcagtgtttagtaattcaaaaatttaaactctgaatttctaaatgcttgttttttga
gtagtagga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAACGTGCACATTAGGCGATAATTG 507 125 1940 Antisense
AATTTATTTATGTTCTATACGTCGT 143 175 1982 Antisense
TGTTCTATACGTCGTGTTTCCCATT 541 561 1992 Antisense
CTTCTAGGCCTTCCATGATTCACAA 361 221 2018 Antisense
GATTCACAATTTTTCGATGCCATAT 377 431 2034 Antisense
ATATCAATTTAGTTGGCCATCTACA 338 19 2055 Antisense
GTTGGCCATCTACATTAAATTACTG 338 435 2066 Antisense
ACTGATATGTTGATGCTATTTTCTA 62 99 2087 Antisense
GCAGATGTCAGTGTTTAGTAATTCA 703 311 2116 Antisense
AACTCTGAATTTCTAAATGCTTGTT 499 141 2149 Antisense
AATGCTTGTTTTTTGAGTAGTAGGA 552 165 2164 Antisense
Sequence Source Affymetrix Proprietary Database