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[173798_at] endothelin converting enzyme 1

Description C47810 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T16A9.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C47810 /WB_GENE_ID=T16A9.4 /WP=CE18259 /CHR=5 /FEA=Genomic Cluster /DEF=endothelin converting enzyme 1; C.elegans cDNA clone yk452c2 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 29      6grp_highest_nonorm > 90      6grp_total > 28      4grp_total > 22      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 274
1 2 3 4 range
A 337.2
(3)
P 		103.7
(-3)
M 		125
(-2.5)
P 		240.1
(0.5)
P 		234
B 193.8
(0.5)
P 		140.8
(-0.9)
M 		63.6
(-3)
P 		288.4
(3)
P 		225
C 178.1
(3)
P 		97.3
(-3)
A 		116.8
(-1.6)
P 		161
(1.7)
P 		81
avr 236.4
(3) 		113.9
(-2.5) 		101.8
(-3) 		229.8
(2.7) 		135

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 165
1 2 3 4 5 6 range
A 147.1
(-0)
P 		230.1
(3)
P 		65.6
(-3)
P 		137.1
(-0.4)
P 		120.7
(-1)
P 		162.7
(0.5)
P 		165
B 230.9
(3)
P 		149.4
(-0.2)
P 		79
(-3)
P 		82.8
(-2.8)
P 		154
(-0)
P 		114.8
(-1.6)
P 		152
C 186.5
(3)
P 		85.1
(-3)
P 		133.8
(-0.1)
P 		129.8
(-0.4)
P 		136.6
(0)
P 		98.6
(-2.2)
P 		101
avr 188.2
(3) 		154.9
(0.9) 		92.8
(-3) 		116.6
(-1.5) 		137.1
(-0.2) 		125.4
(-0.9) 		95
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 108
1 2 3 4 range
A 122.8
(3)
P 		22.5
(-3)
M 		32.7
(-2.4)
P 		35.1
(-2.2)
P 		100
B 61.8
(3)
P 		34.8
(-0.4)
M 		14.7
(-3)
P 		61.4
(2.9)
P 		47
C 59
(3)
P 		28
(-2.8)
A 		40.7
(-0.5)
P 		27.2
(-3)
P 		32
avr 81.2
(3) 		28.4
(-3) 		29.4
(-2.9) 		41.2
(-1.5) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 27.1
(-1.9)
P 		81.8
(3)
P 		14.3
(-3)
P 		34.8
(-1.2)
P 		36.4
(-1)
P 		39.6
(-0.8)
P 		68
B 80.3
(3)
P 		49.7
(-0)
P 		19.2
(-3)
P 		21.9
(-2.7)
P 		62.6
(1.3)
P 		42.8
(-0.7)
P 		61
C 69.1
(3)
P 		26
(-3)
P 		34
(-1.9)
P 		26.1
(-3)
P 		44.2
(-0.5)
P 		33
(-2)
P 		43
avr 58.8
(3) 		52.5
(2) 		22.5
(-3) 		27.6
(-2.2) 		47.7
(1.2) 		38.5
(-0.4) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173798_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25444
Sequence Type Exemplar sequence
Representative Public ID C47810 NCBI
Target Description C47810 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T16A9.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C47810 /WB_GENE_ID=T16A9.4 /WP=CE18259 /CHR=5 /FEA=Genomic Cluster /DEF=endothelin converting enzyme 1; C.elegans cDNA clone yk452c2 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074119(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074119 NCBI Caenorhabditis elegans T16A9.4 (T16A9.4) mRNA, complete cds. 11/11 None
T16A9.4 ENSEMBL cdna:known chromosome:CEL140:V:14217965:14223290:-1 gene:T16A9.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14218004-14223055(-) 100.0 100.0
Public Domain and Genome References
Gene Title endothelin converting enzyme 1
Ensembl T16A9.4
Entrez Gene 179925 Entrez gene
SwissProt Q22523 EMBL-EBI
WormBase CE18259 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB00879 Hypothetical protein T16A9.4 [Caenorhabditis elegans] ref|NP_506520.1| T16A9.4 [Caenorhabditis elegans] sp|Q22523|YCYL_CAEEL Hypothetical zinc metalloproteinase T16A9.4 0.0
blast CAE61001 Hypothetical protein CBG04739 [Caenorhabditis briggsae] 0.0
blast BAB33300.1 neutral endopeptidase 24.11 [Bombyx mori] 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR008753 EMBL-EBI Peptidase M13 5.5E-45
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.5E-70
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506520.1 1 27-49
T16A9.4 1 27-49
Sequence
Target Sequence 
>C. ELEGANS:173798_AT
ttgtattatacattttcattttccccaaattataacttctcgcaaccggttttctcaatg
tgcaacaaaaagagttcagtgactttttcaaaatttcccgccaaagttgatttttttcca
aatcatcttcccattgctttattaatttttccccgagtgtgttctataattgtttcaacg
aattttctcttattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGTATTATACATTTTCATTTTCCC 76 707 2345 Antisense
TTTCCCCAAATTATAACTTCTCGCA 277 553 2364 Antisense
CAAATTATAACTTCTCGCAACCGGT 451 193 2370 Antisense
AAGAGTTCAGTGACTTTTTCAAAAT 21 155 2414 Antisense
TCAAAATTTCCCGCCAAAGTTGATT 273 411 2432 Antisense
TTTTTCCAAATCATCTTCCCATTGC 651 673 2457 Antisense
TGCTTTATTAATTTTTCCCCGAGTG 571 577 2479 Antisense
TAATTTTTCCCCGAGTGTGTTCTAT 554 633 2487 Antisense
TTTCCCCGAGTGTGTTCTATAATTG 657 669 2492 Antisense
GAGTGTGTTCTATAATTGTTTCAAC 497 401 2499 Antisense
TGTTTCAACGAATTTTCTCTTATTT 135 561 2515 Antisense
Sequence Source Affymetrix Proprietary Database