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[173794_at] Phosphorylase kinase

Description CEK016DYR_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C14B9.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D27279 /WB_GENE_ID=C14B9.8 /WP=CE26870 /CHR=3 /FEA=Genomic Cluster /DEF=Phosphorylase kinase; C.elegans cDNA clone yk16d11 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 42      6grp_high1_10_10 > 25      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 20      6grp_total > 25      4grp_total > 47      6grp_highest_sub > 02      6grp_cgc > 33      6grp_high1_sub > 01_02      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 796
1 2 3 4 range
A 1447.7
(3)
P 		823.9
(-3)
P 		1019.4
(-1.1)
P 		982.1
(-1.5)
P 		624
B 1562.3
(3)
P 		898.6
(-2)
P 		855.3
(-2.3)
P 		766.1
(-3)
P 		796
C 1301.8
(3)
P 		1175.1
(0.7)
P 		1037.3
(-1.7)
P 		966.9
(-3)
P 		335
avr 1437.3
(3) 		965.9
(-2.3) 		970.7
(-2.3) 		905
(-3) 		532

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1109
1 2 3 4 5 6 range
A 1356.1
(3)
P 		1020.2
(0.6)
P 		1043.1
(0.7)
P 		809.3
(-1)
P 		836.2
(-0.8)
P 		532.2
(-3)
P 		824
B 1300.4
(0.9)
P 		1641
(3)
P 		682.1
(-3)
P 		1155.5
(-0)
P 		781.4
(-2.4)
P 		1093.6
(-0.4)
P 		959
C 1102.7
(0.7)
P 		1447.7
(3)
P 		961.1
(-0.3)
P 		670.7
(-2.2)
P 		558.3
(-3)
P 		820.3
(-1.2)
P 		889
avr 1253.1
(1.9) 		1369.6
(3) 		895.4
(-1.4) 		878.5
(-1.6) 		725.3
(-3) 		815.4
(-2.2) 		644
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 383
1 2 3 4 range
A 527.1
(3)
P 		178.8
(-2.5)
P 		266.5
(-1.1)
P 		143.8
(-3)
P 		383
B 498.2
(3)
P 		221.9
(-2)
P 		198.2
(-2.4)
P 		163.3
(-3)
P 		335
C 430.9
(3)
P 		337.8
(0.9)
P 		361.5
(1.4)
P 		163
(-3)
P 		268
avr 485.4
(3) 		246.2
(-1.4) 		275.4
(-0.8) 		156.7
(-3) 		329

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 417
1 2 3 4 5 6 range
A 250
(0.1)
P 		362.8
(3)
P 		227.2
(-0.5)
P 		205.3
(-1.1)
P 		252.3
(0.2)
P 		129.6
(-3)
P 		233
B 452.5
(1.5)
P 		546.2
(3)
P 		165.6
(-3)
P 		306.1
(-0.8)
P 		317.5
(-0.6)
P 		407.8
(0.8)
P 		381
C 408.7
(2.4)
P 		441.7
(3)
P 		244.6
(-0.9)
P 		134.9
(-3)
P 		180.6
(-2.1)
P 		274.6
(-0.3)
P 		307
avr 370.4
(1) 		450.2
(3) 		212.5
(-3) 		215.4
(-2.9) 		250.1
(-2.1) 		270.7
(-1.5) 		238
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173794_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24051
Sequence Type Exemplar sequence
Representative Public ID CEK016DYR NCBI
Target Description CEK016DYR_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C14B9.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D27279 /WB_GENE_ID=C14B9.8 /WP=CE26870 /CHR=3 /FEA=Genomic Cluster /DEF=Phosphorylase kinase; C.elegans cDNA clone yk16d11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066376(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066376 NCBI Caenorhabditis elegans C14B9.8 (C14B9.8) mRNA, complete cds. 11/11 None
C14B9.8 ENSEMBL cdna:known chromosome:CEL140:III:8139546:8144534:-1 gene:C14B9.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8139552-8144714(-) 100.0 100.0
Public Domain and Genome References
Gene Title Phosphorylase kinase
Ensembl C14B9.8
Entrez Gene 176149 Entrez gene
SwissProt P34335 EMBL-EBI
WormBase CE38273 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1605908_AT similar to Phosphorylase b kinase alpha regulatory chain, liver isoform (Phosphorylase kinase alpha L subunit) cfa
CANINE_2:CFA.5653.1.A1_AT similar to Phosphorylase b kinase alpha regulatory chain, liver isoform (Phosphorylase kinase alpha L subunit) cfa
CANINE_2:CFAAFFX.20064.1.S1_S_AT similar to Phosphorylase b kinase alpha regulatory chain, liver isoform (Phosphorylase kinase alpha L subunit) cfa
DROSOPHILA_2:1641396_A_AT dm
DROSGENOME1:142687_AT dm
CHICKEN:GGA.16730.1.S1_AT similar to Phosphorylase B kinase alpha regulatory chain, liver isoform (Phosphorylase kinase alpha L subunit) gga
CHICKEN:GGAAFFX.10567.1.S1_S_AT similar to Phosphorylase B kinase alpha regulatory chain, liver isoform (Phosphorylase kinase alpha L subunit) gga
HG-U133_PLUS_2:209439_S_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U133A:209439_S_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U133A_2:209439_S_AT phosphorylase kinase, alpha 2 (liver) hs
HUGENEFL:X80497_AT phosphorylase kinase, alpha 2 (liver) hs
U133_X3P:G1304117_3P_A_AT phosphorylase kinase, alpha 2 (liver) hs
U133_X3P:HS2.414737.1.S1_3P_S_AT phosphorylase kinase, alpha 2 (liver) hs
U133_X3P:HS2.233163.1.S1_3P_AT phosphorylase kinase, alpha 2 (liver) hs
U133_X3P:HS.54941.0.S2_3P_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U133_PLUS_2:1559705_S_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U133_PLUS_2:1560358_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U133A_2:209438_AT phosphorylase kinase, alpha 2 (liver) hs
HG-FOCUS:209438_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U133_PLUS_2:209438_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U133A:209438_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U95AV2:36480_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U95D:67799_AT phosphorylase kinase, alpha 2 (liver) hs
HG-U95E:73083_AT Phosphorylase kinase, alpha 2 (liver) hs
MOE430A:1434518_AT phosphorylase kinase alpha 2 mm
MOUSE430A_2:1434518_AT phosphorylase kinase alpha 2 mm
MOUSE430_2:1434518_AT phosphorylase kinase alpha 2 mm
MOE430A:1430492_AT phosphorylase kinase alpha 2 mm
MOUSE430A_2:1430492_AT phosphorylase kinase alpha 2 mm
MOUSE430_2:1430492_AT phosphorylase kinase alpha 2 mm
MU11KSUBB:MSA.34023.0_S_AT phosphorylase kinase alpha 2 mm
MU11KSUBB:MSA.31221.0_S_AT phosphorylase kinase alpha 2 mm
MG-U74AV2:104003_AT phosphorylase kinase alpha 2 mm
MOE430B:1437941_AT phosphorylase kinase alpha 2 mm
MOUSE430_2:1437941_AT phosphorylase kinase alpha 2 mm
MOE430B:1441882_AT Phosphorylase kinase alpha 2, mRNA (cDNA clone IMAGE:6465984) mm
MOUSE430_2:1441882_AT Phosphorylase kinase alpha 2, mRNA (cDNA clone IMAGE:6465984) mm
MOE430B:1442147_AT Phosphorylase kinase alpha 2, mRNA (cDNA clone IMAGE:6465984) mm
MOUSE430_2:1442147_AT Phosphorylase kinase alpha 2, mRNA (cDNA clone IMAGE:6465984) mm
MOE430B:1443402_AT Phosphorylase kinase alpha 2, mRNA (cDNA clone IMAGE:6465984) mm
MOUSE430_2:1443402_AT Phosphorylase kinase alpha 2, mRNA (cDNA clone IMAGE:6465984) mm
MU19KSUBB:TC32673_AT Phosphorylase kinase alpha 2, mRNA (cDNA clone IMAGE:6465984) mm
Protein Similarities
Method ID Description E-Value
blast AAA27954 Hypothetical protein C14B9.8 [Caenorhabditis elegans] ref|NP_498777.2| C14B9.8 [Caenorhabditis elegans] sp|P34335|KPBA_CAEEL Probable phosphorylase b kinase alpha regulatory chain (Phosphorylase kinase alpha subunit) 0.0
blast CAE70202 Hypothetical protein CBG16677 [Caenorhabditis briggsae] 0.0
blast XP_623102 PREDICTED: similar to ENSANGP00000001572 [Apis mellifera] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.0E-126
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.0E-126
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:173794_AT
attcttgatttctattatatgtacctacttcttccatcatctgcataatcttctcgtcct
ttgtttttttttaatattttcctgtgaattttgctcataagtttaggcgttttggaaaaa
aatctacagtctagaaatttcgagatttatcatcaattttcgtgtaaagttctattatca
tttgtttttctcacagccatcaccaatggtctagtcttgcatcttgccatttttctaatt
tgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCTTGATTTCTATTATATGTACC 596 11 3780 Antisense
TATATGTACCTACTTCTTCCATCAT 473 661 3795 Antisense
TACTTCTTCCATCATCTGCATAATC 470 645 3805 Antisense
TTCCATCATCTGCATAATCTTCTCG 1 701 3811 Antisense
TTTCCTGTGAATTTTGCTCATAAGT 93 669 3857 Antisense
TGCTCATAAGTTTAGGCGTTTTGGA 625 577 3871 Antisense
TCATCAATTTTCGTGTAAAGTTCTA 54 619 3929 Antisense
AAGTTCTATTATCATTTGTTTTTCT 320 155 3946 Antisense
TTCTCACAGCCATCACCAATGGTCT 601 691 3967 Antisense
CAATGGTCTAGTCTTGCATCTTGCC 297 183 3983 Antisense
GCATCTTGCCATTTTTCTAATTTGT 529 309 3998 Antisense
Sequence Source Affymetrix Proprietary Database