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[173785_at] DNA binding protein

Description C49162 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T08D10.1 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C49162 /WB_GENE_ID=T08D10.1 /WP=CE03642 /CHR=X /FEA=Genomic Cluster /DEF=DNA binding protein; C.elegans cDNA clone yk468e11 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 85      6grp_high1_10_10 > 56      4grp_high_8_8 > 61      6grp_highest_nonorm > 91      6grp_total > 57      4grp_total > 59      6grp_highest_sub > Cu      6grp_cgc > 27      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 228
1 2 3 4 range
A 163.4
(-2.9)
P 		329.8
(3)
P 		216.7
(-1)
P 		160.8
(-3)
A 		169
B 301.4
(0)
P 		213.6
(-3)
P 		388.7
(3)
P 		292
(-0.3)
P 		175
C 384.4
(3)
P 		336.9
(1.5)
P 		356.5
(2.1)
P 		195.8
(-3)
P 		189
avr 283.1
(0.8) 		293.4
(1.4) 		320.6
(3) 		216.2
(-3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 284
1 2 3 4 5 6 range
A 332.5
(3)
P 		235.9
(-0.8)
P 		178.3
(-3)
P 		178.4
(-3)
P 		293
(1.5)
P 		293.4
(1.5)
P 		154
B 284.1
(3)
P 		260.1
(1.7)
P 		259.3
(1.6)
P 		200.3
(-1.6)
P 		174.4
(-3)
P 		253.4
(1.3)
P 		110
C 95.7
(-3)
P 		310.3
(1.5)
P 		138.2
(-2.1)
P 		288
(1.1)
P 		116
(-2.6)
P 		380.1
(3)
P 		284
avr 237.4
(-0.7) 		268.8
(0.9) 		191.9
(-3) 		222.2
(-1.4) 		194.5
(-2.9) 		309
(3) 		117
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 104
1 2 3 4 range
A 59.5
(1.5)
P 		71.6
(3)
P 		56.7
(1.1)
P 		23.5
(-3)
A 		48
B 96.1
(3)
P 		52.7
(-3)
P 		90.1
(2.2)
P 		62.2
(-1.7)
P 		43
C 127.3
(3)
P 		96.9
(1.1)
P 		124.3
(2.8)
P 		33
(-3)
P 		94
avr 94.3
(3) 		73.7
(0.7) 		90.4
(2.6) 		39.6
(-3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 61.3
(-0.3)
P 		83.9
(2.5)
P 		38.8
(-3)
P 		45.3
(-2.2)
P 		88.4
(3)
P 		71.4
(0.9)
P 		50
B 98.9
(3)
P 		86.6
(1.4)
P 		63
(-1.7)
P 		53.1
(-3)
P 		70.9
(-0.7)
P 		94.5
(2.4)
P 		46
C 35.5
(-3)
P 		94.7
(0.9)
P 		35.2
(-3)
P 		57.9
(-1.5)
P 		37.5
(-2.9)
P 		127.2
(3)
P 		92
avr 65.2
(-0.8) 		88.4
(1.9) 		45.7
(-3) 		52.1
(-2.3) 		65.6
(-0.7) 		97.7
(3) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173785_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25993
Sequence Type Exemplar sequence
Representative Public ID C49162 NCBI
Target Description C49162 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T08D10.1 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C49162 /WB_GENE_ID=T08D10.1 /WP=CE03642 /CHR=X /FEA=Genomic Cluster /DEF=DNA binding protein; C.elegans cDNA clone yk468e11 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077598(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077598 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-295) (tag-295) mRNA, complete cds. 10/11 None
T08D10.1 ENSEMBL cdna:known chromosome:CEL140:X:11944350:11946755:-1 gene:T08D10.1 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11944303-11946724(-) 100.0 100.0
Public Domain and Genome References
Gene Title DNA binding protein
Gene Symbol tag-295
Ensembl T08D10.1
Entrez Gene 181368 Entrez gene
SwissProt Q20858 EMBL-EBI
WormBase CE03642 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA90639 Hypothetical protein T08D10.1 [Caenorhabditis elegans] emb|CAA90612.1| Hypothetical protein T08D10.1 [Caenorhabditis elegans] ref|NP_509999.1| Temporarily Assigned Gene name family member (tag-295) [Caenorhabditis elegans] 0.0
blast CAE69400 Hypothetical protein CBG15536 [Caenorhabditis briggsae] 4.0E-62
blast CAH60767 Hypothetical protein Y53H1A.5 [Caenorhabditis elegans] ref|NP_001021787.1| Y53H1A.5 [Caenorhabditis elegans] 3.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR001289 EMBL-EBI CCAAT-binding transcription factor, subunit B 4.4E-43
Sequence
Target Sequence 
>C. ELEGANS:173785_AT
gcttaatgtgtattgcaaccgttgccaaacaaaattcacctcaccaaaatcattcatgtt
ccctacgctttttgccacagaaatgtaccttctcttattgaccgatcttttcttcgggat
tatgcccatttctctttcaatgttttcaaattttttatcctcattatgcattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTTAATGTGTATTGCAACCGTTGC 369 305 1474 Antisense
GTTGCCAAACAAAATTCACCTCACC 52 441 1494 Antisense
AATTCACCTCACCAAAATCATTCAT 303 175 1506 Antisense
AAAATCATTCATGTTCCCTACGCTT 348 131 1519 Antisense
CCTACGCTTTTTGCCACAGAAATGT 198 269 1535 Antisense
GAAATGTACCTTCTCTTATTGACCG 267 361 1553 Antisense
CTTCTCTTATTGACCGATCTTTTCT 379 221 1562 Antisense
CGATCTTTTCTTCGGGATTATGCCC 441 249 1576 Antisense
CGGGATTATGCCCATTTCTCTTTCA 187 245 1588 Antisense
GCCCATTTCTCTTTCAATGTTTTCA 417 287 1597 Antisense
AATTTTTTATCCTCATTATGCATTT 326 175 1622 Antisense
Sequence Source Affymetrix Proprietary Database