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[173784_at] folate transporter

Description CEC5041 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C50E3.11 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C08504 /WB_GENE_ID=C50E3.11 /WP=CE08904 /CHR=5 /FEA=Genomic Cluster /DEF=folate transporter; C.elegans cDNA clone yk107g11 : 5prime end, single read.
Cluster 6grp_total > 87      4grp_total > 04      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 27.8
(-1)
A 		29.2
(-0.9)
A 		3.4
(-3)
A 		76.2
(3)
P 		73
B 54.9
(3)
A 		8.9
(-2.5)
A 		38.9
(1.1)
A 		5
(-3)
A 		50
C 6.3
(-3)
A 		30.1
(-1)
A 		23
(-1.6)
A 		76.1
(3)
A 		70
avr 29.7
(-1.5) 		22.7
(-2.8) 		21.8
(-3) 		52.4
(3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 200
1 2 3 4 5 6 range
A 40.7
(-1.4)
A 		5.5
(-3)
A 		28.1
(-2)
P 		58.2
(-0.6)
P 		102.7
(1.5)
P 		135.6
(3)
P 		130
B 34.9
(-3)
A 		44.8
(-2.6)
A 		35.5
(-3)
A 		50.9
(-2.4)
A 		104
(-0.2)
P 		185.6
(3)
P 		151
C 33.3
(-2.9)
A 		35.4
(-2.9)
A 		31.5
(-3)
A 		34.8
(-2.9)
A 		105
(-0.5)
P 		205.3
(3)
P 		174
avr 36.3
(-2.7) 		28.6
(-3) 		31.7
(-2.9) 		48
(-2.2) 		103.9
(0.1) 		175.5
(3) 		147
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 10.1
(2.4)
A 		6.3
(0.1)
A 		0.9
(-3)
A 		11.2
(3)
P 		10
B 17.5
(3)
A 		2.2
(-2.6)
A 		9
(-0.1)
A 		1.1
(-3)
A 		16
C 2.1
(-3)
A 		8.7
(0.7)
A 		8
(0.3)
A 		12.8
(3)
A 		11
avr 9.9
(3) 		5.7
(-3) 		6
(-2.6) 		8.4
(0.9) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 67
1 2 3 4 5 6 range
A 7.5
(-1.9)
A 		1.9
(-3)
A 		6.1
(-2.2)
P 		14.8
(-0.5)
P 		31
(2.6)
P 		33
(3)
P 		31
B 12.1
(-2.7)
A 		14.9
(-2.4)
A 		8.6
(-3)
A 		13.5
(-2.5)
A 		42.3
(0.3)
P 		69.2
(3)
P 		61
C 12.3
(-2.5)
A 		10.8
(-2.6)
A 		8
(-2.9)
A 		7
(-3)
A 		34
(-0.4)
P 		68.7
(3)
P 		62
avr 10.6
(-2.6) 		9.2
(-2.8) 		7.6
(-3) 		11.8
(-2.5) 		35.8
(0.4) 		57
(3) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173784_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25266
Sequence Type Exemplar sequence
Representative Public ID CEC5041 NCBI
Target Description CEC5041 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C50E3.11 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C08504 /WB_GENE_ID=C50E3.11 /WP=CE08904 /CHR=5 /FEA=Genomic Cluster /DEF=folate transporter; C.elegans cDNA clone yk107g11 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072563(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072563 NCBI Caenorhabditis elegans C50E3.11 (C50E3.11) mRNA, complete cds. 11/11 None
C50E3.11 ENSEMBL cdna:known chromosome:CEL140:V:7604915:7612330:-1 gene:C50E3.11 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000028536 6/11 Cross Hyb Matching Probes None
GENEFINDER00000028564 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7604926-7612332(-) 100.0 100.0
Public Domain and Genome References
Gene Title folate transporter
Ensembl C50E3.11
Entrez Gene 179144 Entrez gene
SwissProt Q22931 EMBL-EBI
WormBase CE08904 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5542 folic acid binding inferred from electronic annotation QuickGO AmiGO
8518 reduced folate carrier activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_504964 C50E3.11 [Caenorhabditis elegans] gb|AAB04886.1| Hypothetical protein C50E3.11 [Caenorhabditis elegans] sp|Q22931|FOLT2_CAEEL Folate-like transporter 0.0
blast CAE70448 Hypothetical protein CBG17027 [Caenorhabditis briggsae] 1.0E-169
blast CAE58079 Hypothetical protein CBG01160 [Caenorhabditis briggsae] 1.0E-158
Protein Domains
Database ID Description E-Value
Pfam IPR002666 EMBL-EBI Reduced folate carrier 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504964.1 7 44-66,81-100,113-135,139-161,212-231,294-316,337-359
C50E3.11 7 44-66,81-100,113-135,139-161,212-231,294-316,337-359
Sequence
Target Sequence 
>C. ELEGANS:173784_AT
tctacggatgcggattctgattatgtgactgatgacaaaactggaactgcaaatattgaa
aaaccgccaaaaaacaatttgaagcgcaagaacaatagaaaagacgatgacgaggaggat
ggtcccagtgcgaaaatcccgtcaatctccacaggccggccaactgtagcctaaattttg
tatattttataatacttgactcttccttcctcgaacgtattctcatcatccatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTACGGATGCGGATTCTGATTATG 231 615 1624 Antisense
GGATTCTGATTATGTGACTGATGAC 696 507 1635 Antisense
GAACTGCAAATATTGAAAAACCGCC 631 347 1667 Antisense
TGACGAGGAGGATGGTCCCAGTGCG 678 563 1731 Antisense
GGATGGTCCCAGTGCGAAAATCCCG 224 513 1740 Antisense
CCCAGTGCGAAAATCCCGTCAATCT 576 257 1747 Antisense
AGGCCGGCCAACTGTAGCCTAAATT 252 1 1776 Antisense
AACTGTAGCCTAAATTTTGTATATT 253 143 1785 Antisense
TTATAATACTTGACTCTTCCTTCCT 30 681 1810 Antisense
CTTCCTCGAACGTATTCTCATCATC 579 219 1829 Antisense
CTCGAACGTATTCTCATCATCCATT 36 233 1833 Antisense
Sequence Source Affymetrix Proprietary Database