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[173783_at] Aminopeptidase

Description CEK013BXR_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T16G12.1 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D32390 /WB_GENE_ID=T16G12.1 /WP=CE26001 /CHR=3 /FEA=Genomic Cluster /DEF=Aminopeptidase; C.elegans cDNA clone yk13b10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 22      4grp_high_8_8 > 57      6grp_highest_nonorm > 41      6grp_total > 22      4grp_total > 62      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1404
1 2 3 4 range
A 1450.1
(3)
P 		824.4
(0.2)
P 		1193.1
(1.9)
P 		95.2
(-3)
A 		1355
B 1341.7
(3)
P 		879.5
(0.9)
P 		769.3
(0.3)
P 		45.9
(-3)
A 		1296
C 1185.7
(3)
P 		478.3
(-0.9)
P 		875.9
(1.3)
P 		91
(-3)
P 		1095
avr 1325.8
(3) 		727.4
(0.1) 		946.1
(1.2) 		77.4
(-3) 		1248

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 880
1 2 3 4 5 6 range
A 820.2
(1.1)
P 		1096.7
(3)
P 		811.2
(1)
P 		575.4
(-0.7)
P 		365.1
(-2.1)
P 		240.7
(-3)
P 		856
B 749.1
(1.3)
P 		931.6
(3)
P 		478
(-1.2)
P 		648.4
(0.4)
P 		444.9
(-1.5)
P 		281.1
(-3)
P 		651
C 820.6
(1.8)
P 		805.9
(1.7)
P 		970.7
(3)
P 		421.3
(-1.4)
P 		414.9
(-1.4)
P 		217.1
(-3)
P 		754
avr 796.6
(1.7) 		944.7
(3) 		753.3
(1.4) 		548.4
(-0.4) 		408.3
(-1.6) 		246.3
(-3) 		698
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 518
1 2 3 4 range
A 528
(3)
P 		179
(-1.1)
P 		311.9
(0.5)
P 		13.9
(-3)
A 		514
B 427.9
(3)
P 		217.2
(-0)
P 		178.3
(-0.6)
P 		9.8
(-3)
A 		418
C 392.5
(3)
P 		137.5
(-1.1)
P 		305.3
(1.6)
P 		15.4
(-3)
P 		377
avr 449.5
(3) 		177.9
(-0.7) 		265.2
(0.5) 		13
(-3) 		437

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 331
1 2 3 4 5 6 range
A 151.2
(-1.3)
P 		390
(3)
P 		176.7
(-0.9)
P 		145.9
(-1.4)
P 		110.2
(-2.1)
P 		58.6
(-3)
P 		331
B 260.6
(1.6)
P 		310.1
(3)
P 		116.1
(-2.7)
P 		171.8
(-1)
P 		180.7
(-0.8)
P 		104.8
(-3)
P 		205
C 304.2
(3)
P 		245.9
(1.5)
P 		247.1
(1.5)
P 		84.7
(-2.7)
P 		134.2
(-1.4)
P 		72.7
(-3)
P 		232
avr 238.7
(1.1) 		315.3
(3) 		180
(-0.4) 		134.1
(-1.6) 		141.7
(-1.4) 		78.7
(-3) 		237
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173783_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23850
Sequence Type Exemplar sequence
Representative Public ID CEK013BXR NCBI
Target Description CEK013BXR_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T16G12.1 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D32390 /WB_GENE_ID=T16G12.1 /WP=CE26001 /CHR=3 /FEA=Genomic Cluster /DEF=Aminopeptidase; C.elegans cDNA clone yk13b10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066829(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066829 NCBI Caenorhabditis elegans T16G12.1 (Aminopeptidase) mRNA, complete cds. 11/11 None
GENEFINDER00000010177 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10038512:10050731:-1 10/11 None
SNAP00000010162 ENSEMBL cdna:SNAP chromosome:CEL140:III:10040140:10048601:-1 11/11 None
T16G12.1 ENSEMBL cdna:known chromosome:CEL140:III:10040080:10049670:-1 gene:T16G12.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10040086-10049629(-) 99.74 100.0
Public Domain and Genome References
Gene Title Aminopeptidase
Ensembl T16G12.1
Entrez Gene 176416 Entrez gene
SwissProt Q22531 EMBL-EBI
WormBase CE36716 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4179 membrane alanyl aminopeptidase activity inferred from electronic annotation QuickGO AmiGO
5507 copper ion binding inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA82971 Hypothetical protein T16G12.1 [Caenorhabditis elegans] ref|NP_499230.2| T16G12.1 [Caenorhabditis elegans] 0.0
blast CAE65056 Hypothetical protein CBG09901 [Caenorhabditis briggsae] 0.0
blast CAA82973 Hypothetical protein T16G12.7 [Caenorhabditis elegans] emb|CAA83616.1| Hypothetical protein T16G12.7 [Caenorhabditis elegans] ref|NP_499229.1| T16G12.7 [Caenorhabditis elegans] 1.0E-171
blast XP_393122 PREDICTED: similar to CG14516-PA, isoform A, partial [Apis mellifera] 1.0E-124
Protein Domains
Database ID Description E-Value
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 4.0E-39
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 1.0E-126
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 1.0E-126
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 1.0E-126
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 1.0E-126
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 1.0E-126
Pfam IPR001930 EMBL-EBI Peptidase M1, membrane alanine aminopeptidase 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499230.2 1 5-24
T16G12.1 1 5-24
Sequence
Target Sequence 
>C. ELEGANS:173783_AT
tccgtttgacttctctctatacgtcaaagccatgttccaaaattggtcaacttcaaacca
actagaattggcaaaggatttccagaattcttccaattataatcttcttaatgcggctca
aaagaacatttataaacaaggaattttaacagttaatagcaatgccaactggatgttgat
ctacaaaactccgttccttgagtggattcagaagaattttggagcacctaatttgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCGTTTGACTTCTCTCTATACGTC 248 595 5453 Antisense
GACTTCTCTCTATACGTCAAAGCCA 37 371 5460 Antisense
ATACGTCAAAGCCATGTTCCAAAAT 283 23 5471 Antisense
GGTCAACTTCAAACCAACTAGAATT 277 501 5497 Antisense
GGATTTCCAGAATTCTTCCAATTAT 6 511 5528 Antisense
AATTCTTCCAATTATAATCTTCTTA 625 175 5538 Antisense
ATAATCTTCTTAATGCGGCTCAAAA 653 25 5551 Antisense
AGCAATGCCAACTGGATGTTGATCT 629 75 5610 Antisense
GTTGATCTACAAAACTCCGTTCCTT 553 437 5627 Antisense
AACTCCGTTCCTTGAGTGGATTCAG 51 143 5639 Antisense
AGAATTTTGGAGCACCTAATTTGTA 6 21 5665 Antisense
Sequence Source Affymetrix Proprietary Database