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[173772_at]

Description C45838 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C16D9.2 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C45838 /WB_GENE_ID=C16D9.2 /WP=CE06838 /CHR=5 /FEA=Genomic Cluster /DEF=tyrosine-protein kinase; C.elegans cDNA clone yk402e9 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 01      4grp_highest_8_8 > 32      4grp_high_8_8 > 50      6grp_highest_nonorm > 90      6grp_total > 13      4grp_total > 61      6grp_highest_sub > De      6grp_cgc > 30      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 129
1 2 3 4 range
A 184.4
(3)
P 		164.4
(1.8)
P 		177.1
(2.6)
P 		85.2
(-3)
P 		99
B 189.6
(3)
P 		171.2
(1.9)
P 		113.1
(-1.7)
P 		91
(-3)
P 		99
C 199.6
(3)
P 		179.8
(2.1)
P 		185.1
(2.3)
P 		70.6
(-3)
P 		129
avr 191.2
(3) 		171.8
(1.9) 		158.4
(1.2) 		82.3
(-3) 		109

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 181
1 2 3 4 5 6 range
A 223.1
(3)
P 		198.7
(2)
P 		171.8
(0.8)
P 		131
(-0.9)
P 		108.7
(-1.9)
P 		81.8
(-3)
P 		141
B 251
(3)
P 		178.4
(0.3)
P 		144.5
(-0.9)
P 		232.4
(2.3)
P 		119.2
(-1.8)
P 		87.3
(-3)
P 		164
C 237.6
(3)
P 		199.8
(1.6)
P 		186.6
(1.2)
P 		162.2
(0.3)
P 		105.6
(-1.7)
P 		70.4
(-3)
P 		167
avr 237.2
(3) 		192.3
(1.3) 		167.6
(0.3) 		175.2
(0.6) 		111.2
(-1.8) 		79.8
(-3) 		157
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 67.1
(3)
P 		35.7
(-0.5)
P 		46.3
(0.7)
P 		12.5
(-3)
P 		55
B 60.4
(3)
P 		42.3
(0.4)
P 		26.2
(-2)
P 		19.4
(-3)
P 		41
C 66.1
(3)
P 		51.7
(1.4)
P 		64.5
(2.8)
P 		11.9
(-3)
P 		54
avr 64.5
(3) 		43.2
(0.4) 		45.7
(0.7) 		14.6
(-3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 41.1
(-0.5)
P 		70.7
(3)
P 		37.4
(-0.9)
P 		33.2
(-1.4)
P 		32.8
(-1.5)
P 		19.9
(-3)
P 		51
B 87.3
(3)
P 		59.4
(-0.1)
P 		35.1
(-2.7)
P 		61.6
(0.2)
P 		48.4
(-1.3)
P 		32.6
(-3)
P 		55
C 88.1
(3)
P 		61
(0.5)
P 		47.5
(-0.8)
P 		32.6
(-2.2)
P 		34.2
(-2)
P 		23.6
(-3)
P 		65
avr 72.2
(3) 		63.7
(1.9) 		40
(-1.1) 		42.5
(-0.8) 		38.5
(-1.3) 		25.4
(-3) 		47
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173772_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25213
Sequence Type Exemplar sequence
Representative Public ID C45838 NCBI
Target Description C45838 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C16D9.2 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C45838 /WB_GENE_ID=C16D9.2 /WP=CE06838 /CHR=5 /FEA=Genomic Cluster /DEF=tyrosine-protein kinase; C.elegans cDNA clone yk402e9 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182327(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182327 NCBI Caenorhabditis elegans ROLler: helically twisted, animals roll when moving family member (rol-3) (rol-3) mRNA, complete cds. 11/11 None
C16D9.2c ENSEMBL cdna:known chromosome:CEL140:V:8230891:8235929:-1 gene:C16D9.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179204 3 1 NM_072721 173773_s_at (11)
NM_182327 173772_at (11), 173773_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8230945-8240943(-) 100.0 100.0
Public Domain and Genome References
Ensembl C16D9.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN84864 Roller: helically twisted, animals roll when moving protein 3, isoform a [Caenorhabditis elegans] ref|NP_505122.3| ROLler: helically twisted, animals roll when moving family member (rol-3) [Caenorhabditis elegans] 0.0
blast AAN84866 Roller: helically twisted, animals roll when moving protein 3, isoform c [Caenorhabditis elegans] ref|NP_872127.1| ROLler: helically twisted, animals roll when moving family member (rol-3) [Caenorhabditis elegans] 0.0
blast CAE56943 Hypothetical protein CBG24788 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003961 EMBL-EBI Fibronectin, type III 2.8E-5
Pfam IPR003961 EMBL-EBI Fibronectin, type III 1.8E-5
Pfam IPR000719 EMBL-EBI Protein kinase 4.8E-17
Pfam IPR000719 EMBL-EBI Protein kinase 8.7E-24
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_872127.1 1 702-724
C16D9.2c 1 702-724
Sequence
Target Sequence 
>C. ELEGANS:173772_AT
atcagtcaagtctagaatccataattaaatatttatagcatgacatcaaagaatctttgt
tttctatctcaactccggtcttctcttcattttcattcacgaccactaattttctttccc
atttggacggttgctaggcgccaattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCAGTCAAGTCTAGAATCCATAAT 648 29 7142 Antisense
AAATATTTATAGCATGACATCAAAG 7 115 7168 Antisense
GAATCTTTGTTTTCTATCTCAACTC 51 329 7192 Antisense
ATCTTTGTTTTCTATCTCAACTCCG 18 33 7194 Antisense
TCTTCTCTTCATTTTCATTCACGAC 540 617 7220 Antisense
TCTCTTCATTTTCATTCACGACCAC 313 613 7223 Antisense
CTTCATTTTCATTCACGACCACTAA 415 221 7226 Antisense
CATTCACGACCACTAATTTTCTTTC 47 217 7235 Antisense
TTTCTTTCCCATTTGGACGGTTGCT 91 671 7252 Antisense
CCCATTTGGACGGTTGCTAGGCGCC 683 257 7259 Antisense
TTGGACGGTTGCTAGGCGCCAATTT 251 683 7264 Antisense
Sequence Source Affymetrix Proprietary Database