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[173771_s_at] importin beta, nuclear transport factor

Description C64804 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C53D5.6 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C64804 /WB_GENE_ID=C53D5.6 /WP=CE26971 /CHR=1 /FEA=Genomic Cluster /DEF=Ran_GTP binding protein; C.elegans cDNA clone yk360c7 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 58      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 68      6grp_total > 48      4grp_total > 02      6grp_highest_sub > Cu      6grp_cgc > 18      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7516
1 2 3 4 range
A 2917.1
(-3)
P 		3062.9
(-2.9)
P 		3087.5
(-2.9)
P 		10042
(3)
P 		7125
B 2795.9
(-3)
P 		2875.2
(-2.9)
P 		2869.4
(-2.9)
P 		9943.7
(3)
P 		7148
C 2526.4
(-3)
P 		2967.9
(-2.6)
P 		3031.1
(-2.5)
P 		8551.1
(3)
P 		6025
avr 2746.5
(-3) 		2968.7
(-2.8) 		2996
(-2.8) 		9512.3
(3) 		6766

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1510
1 2 3 4 5 6 range
A 1914.5
(0.1)
P 		2051
(0.7)
P 		1178.4
(-2.9)
P 		1161.2
(-3)
P 		1693.7
(-0.8)
P 		2617.3
(3)
P 		1456
B 2236.7
(2.1)
P 		1982.1
(0.7)
P 		1307.8
(-3)
P 		1390.5
(-2.5)
P 		1581.6
(-1.5)
P 		2396.4
(3)
P 		1089
C 2437.3
(3)
P 		1915.2
(0.6)
P 		1537.4
(-1.1)
P 		1107.7
(-3)
P 		2024.7
(1.1)
P 		2370.9
(2.7)
P 		1330
avr 2196.2
(1.7) 		1982.8
(0.7) 		1341.2
(-2.4) 		1219.8
(-3) 		1766.7
(-0.4) 		2461.5
(3) 		1242
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1454
1 2 3 4 range
A 1062.1
(-0)
P 		664.9
(-3)
P 		807.1
(-1.9)
P 		1469.9
(3)
P 		805
B 891.6
(-2.1)
P 		710.1
(-2.8)
P 		664.9
(-3)
P 		2119
(3)
P 		1454
C 836.3
(-3)
P 		853.3
(-2.8)
P 		1056.4
(-0.8)
P 		1441.9
(3)
P 		606
avr 930
(-1.8) 		742.8
(-3) 		842.8
(-2.4) 		1676.9
(3) 		934

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 681
1 2 3 4 5 6 range
A 352.9
(-1.8)
P 		729.4
(3)
P 		256.6
(-3)
P 		294.5
(-2.5)
P 		511.1
(0.2)
P 		637.2
(1.8)
P 		473
B 778.3
(1.8)
P 		659.7
(0.6)
P 		317.6
(-3)
P 		368.3
(-2.5)
P 		642.6
(0.4)
P 		893.6
(3)
P 		576
C 903.4
(3)
P 		584.4
(0.2)
P 		391.3
(-1.5)
P 		222.8
(-3)
P 		655.2
(0.8)
P 		793.7
(2)
P 		681
avr 678.2
(1.8) 		657.8
(1.5) 		321.8
(-2.7) 		295.2
(-3) 		603
(0.9) 		774.8
(3) 		480
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173771_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23168
Sequence Type Exemplar sequence
Representative Public ID C64804 NCBI
Target Description C64804 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C53D5.6 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C64804 /WB_GENE_ID=C53D5.6 /WP=CE26971 /CHR=1 /FEA=Genomic Cluster /DEF=Ran_GTP binding protein; C.elegans cDNA clone yk360c7 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058314(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058314 NCBI Caenorhabditis elegans IMportin Beta family member (imb-3) (imb-3) mRNA, complete cds. 11/11 None
C53D5.6.1 ENSEMBL cdna:known chromosome:CEL140:I:310982:314990:-1 gene:C53D5.6 11/11 None
C53D5.6.2 ENSEMBL cdna:known chromosome:CEL140:I:310993:314992:-1 gene:C53D5.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:311353-314569(-) 100.0 100.0
Public Domain and Genome References
Gene Title importin beta, nuclear transport factor
Gene Symbol imb-3
Ensembl C53D5.6
Entrez Gene 3565440 Entrez gene
SwissProt Q9N5V3 EMBL-EBI
WormBase CE26971 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:245941_AT PBS lyase HEAT-like repeat-containing protein at
CANINE:1594347_AT similar to RAN binding protein 5 cfa
CANINE:1588870_AT similar to RAN binding protein 5 cfa
CANINE_2:CFA.10369.1.S1_AT similar to RAN binding protein 5 cfa
CANINE_2:CFA.18531.1.S1_S_AT similar to RAN binding protein 5 cfa
CANINE_2:CFAAFFX.9307.1.S1_S_AT similar to RAN binding protein 5 cfa
DROSOPHILA_2:1623739_AT Karyopherin 3 dm
DROSGENOME1:141618_AT Karyopherin 3 dm
CHICKEN:GGA.17602.1.S1_S_AT RAN binding protein 5 gga
CHICKEN:GGA.2487.1.S1_AT RAN binding protein 5 gga
CHICKEN:GGAAFFX.25111.1.S1_S_AT RAN binding protein 5 gga
HG-U133_PLUS_2:211952_AT RAN binding protein 5 hs
HG-U133A_2:211952_AT RAN binding protein 5 hs
HG-U133A:211952_AT RAN binding protein 5 hs
HG-U133_PLUS_2:211953_S_AT RAN binding protein 5 hs
HG-U133A_2:211953_S_AT RAN binding protein 5 hs
HG-U133A:211953_S_AT RAN binding protein 5 hs
HG-U133_PLUS_2:211955_AT RAN binding protein 5 hs
HG-U133A_2:211955_AT RAN binding protein 5 hs
HG-U133A:211955_AT RAN binding protein 5 hs
HG-U133_PLUS_2:211954_S_AT RAN binding protein 5 hs
HG-U133A_2:211954_S_AT RAN binding protein 5 hs
HG-FOCUS:211954_S_AT RAN binding protein 5 hs
HG-U133A:211954_S_AT RAN binding protein 5 hs
HG-U95AV2:39028_AT RAN binding protein 5 hs
HU35KSUBA:RC_AA056451_AT RAN binding protein 5 hs
HU35KSUBA:RC_AA283922_AT RAN binding protein 5 hs
HUGENEFL:U72761_AT RAN binding protein 5 hs
U133_X3P:HS.113503.0.S4_3P_AT RAN binding protein 5 hs
U133_X3P:HS.113503.0.S3_3P_AT RAN binding protein 5 hs
U133_X3P:HS.113503.0.S3_3P_A_AT RAN binding protein 5 hs
U133_X3P:HS.113503.0.S2_3P_A_AT RAN binding protein 5 hs
U133_X3P:HS.113503.0.S1_3P_AT RAN binding protein 5 hs
U133_X3P:211953_3P_S_AT RAN binding protein 5 hs
HG-U95B:44108_AT RAN binding protein 5 hs
HG-U95E:87848_S_AT RAN binding protein 5 hs
HG-U133_PLUS_2:236906_X_AT RAN binding protein 5 hs
HG-U133B:236906_X_AT RAN binding protein 5 hs
MOE430A:1426945_AT RAN binding protein 5 mm
MOUSE430A_2:1426945_AT RAN binding protein 5 mm
MOUSE430_2:1426945_AT RAN binding protein 5 mm
MOE430A:1426946_AT RAN binding protein 5 mm
MOUSE430_2:1426946_AT RAN binding protein 5 mm
MOUSE430A_2:1426946_AT RAN binding protein 5 mm
MOUSE430A_2:1431706_AT RAN binding protein 5 mm
MOE430A:1431706_AT RAN binding protein 5 mm
MOUSE430_2:1431706_AT RAN binding protein 5 mm
MU11KSUBA:AA409333_RC_S_AT RAN binding protein 5 mm
MU11KSUBA:AA120563_G_AT RAN binding protein 5 mm
MU11KSUBA:AA120563_AT RAN binding protein 5 mm
MG-U74AV2:93070_AT RAN binding protein 5 mm
MG-U74AV2:161757_F_AT RAN binding protein 5 mm
MU19KSUBA:TC24224_AT RAN binding protein 5, mRNA (cDNA clone IMAGE:30464366) mm
MU19KSUBB:TC27232_AT RAN binding protein 5, mRNA (cDNA clone IMAGE:30464366) mm
MU19KSUBB:TC27232_G_AT RAN binding protein 5, mRNA (cDNA clone IMAGE:30464366) mm
MU19KSUBC:TC36343_AT RAN binding protein 5, mRNA (cDNA clone IMAGE:30464366) mm
RAT230_2:1396742_AT karyopherin (importin) beta 3 (predicted) rn
RAE230B:1396742_AT karyopherin (importin) beta 3 (predicted) rn
RG-U34B:RC_AA925863_AT karyopherin (importin) beta 3 (predicted) rn
RG-U34C:RC_AI137586_AT karyopherin (importin) beta 3 (predicted) rn
RAE230A:1373955_AT karyopherin (importin) beta 3 (predicted) rn
RAT230_2:1373955_AT karyopherin (importin) beta 3 (predicted) rn
RAE230B:1391470_AT Karyopherin (importin) beta 3 (predicted) rn
RAT230_2:1391470_AT Karyopherin (importin) beta 3 (predicted) rn
RG-U34C:RC_AI236903_AT Karyopherin (importin) beta 3 (predicted) rn
RG-U34B:RC_AA964226_AT Karyopherin (importin) beta 3 (predicted) rn
YEAST_2:1775694_AT Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p Sc
Protein Similarities
Method ID Description E-Value
blast AAF39782 Importin beta family protein 3 [Caenorhabditis elegans] ref|NP_490715.1| IMportin Beta family member (imb-3) [Caenorhabditis elegans] 0.0
blast CAE74344 Hypothetical protein CBG22062 [Caenorhabditis briggsae] 0.0
blast XP_542647 PREDICTED: similar to RAN binding protein 5 isoform 1 [Canis familiaris] 0.0
Sequence
Target Sequence 
>C. ELEGANS:173771_S_AT
gacaagcagcgagctacggtttcggagttatggccatcaactaccaccagatcagcgact
accgaaacgagattttgagctgtttggagccactggccgccatgattcaacgagaggacg
cccgagccaccgaggagagcactgttgccacggaaaacgcgatttctgcatttgccaaaa
tcatcggcaatgtgccacttcctgctgaagcctacggaaaggtcgtcgagatgttcctga
gctggttgccaacatattcagacaccgaggaaagcccctacatttacacgtgtctcgccg
aattgtttgataaacaagacgccgcactttttggaccagaaaatcagaatcttccgagaa
tcttccttgttggcttgctctcaatcgccaacgatgctttcaacgacgacgatagcggca
accaaaccaagcaaagaattgtcacaatcctgaagcaaatctacgcatcgttcccacaat
tcgcgcagcaagatggaatcgacgagcaccttaccagtgtccttcaacaaactctc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAAGCAGCGAGCTACGGTTTCGG 99 365 2382 Antisense
GGAGTTATGGCCATCAACTACCACC 458 515 2405 Antisense
CTGGCCGCCATGATTCAACGAGAGG 58 241 2474 Antisense
TCGAGATGTTCCTGAGCTGGTTGCC 300 603 2607 Antisense
TTTACACGTGTCTCGCCGAATTGTT 552 671 2664 Antisense
GAATCTTCCGAGAATCTTCCTTGTT 693 327 2728 Antisense
CCAACGATGCTTTCAACGACGACGA 638 275 2769 Antisense
GAAGCAAATCTACGCATCGTTCCCA 84 341 2833 Antisense
TTCCCACAATTCGCGCAGCAAGATG 20 699 2852 Antisense
TGGAATCGACGAGCACCTTACCAGT 221 547 2875 Antisense
TACCAGTGTCCTTCAACAAACTCTC 218 651 2893 Antisense
Sequence Source Affymetrix Proprietary Database