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[173768_at] fucosyltransferase

Description CEK078DYR_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=K08F8.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D71575 /WB_GENE_ID=K08F8.3 /WP=CE03469 /CHR=2 /FEA=Genomic Cluster /DEF=fucosyltransferase; C.elegans cDNA clone yk78d11 : 3prime end, single read.
Cluster 6grp_high1_10_10 > 44      4grp_highest_8_8 > 14      4grp_high_8_8 > 15      6grp_total > 0      4grp_total > 08      6grp_total_sub > Hat      6grp_cgc > 55      6grp_high1_sub > 02      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 868
1 2 3 4 range
A 301.7
(-2.2)
P 		194.5
(-3)
P 		441
(-1.1)
P 		968.9
(3)
P 		774
B 238.9
(-3)
P 		424.3
(-1.6)
P 		458.7
(-1.4)
P 		1062.8
(3)
P 		824
C 445.6
(-3)
P 		510.8
(-1.9)
P 		482.2
(-2.4)
P 		794.6
(3)
P 		349
avr 328.7
(-3) 		376.5
(-2.5) 		460.6
(-1.7) 		942.1
(3) 		613

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 572
1 2 3 4 5 6 range
A 73
(-3)
M 		332.3
(-0)
P 		600.3
(3)
P 		229.4
(-1.2)
P 		384
(0.5)
P 		221.5
(-1.3)
P 		527
B 332.3
(-0.8)
P 		618.7
(2.7)
P 		155.7
(-3)
P 		363.9
(-0.4)
P 		378.2
(-0.3)
P 		644.9
(3)
P 		489
C 225.1
(-1.6)
P 		501.6
(3)
P 		218.7
(-1.7)
P 		142.4
(-3)
P 		289.3
(-0.5)
P 		453.5
(2.2)
P 		359
avr 210.1
(-3) 		484.2
(3) 		324.9
(-0.5) 		245.2
(-2.2) 		350.5
(0.1) 		440
(2) 		274
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 109.8
(1.1)
P 		42.2
(-3)
P 		115.3
(1.4)
P 		141.8
(3)
P 		100
B 76.2
(-3)
P 		104.8
(-1.9)
P 		106.3
(-1.8)
P 		226.5
(3)
P 		150
C 147.5
(-0.6)
P 		146.8
(-0.7)
P 		168.1
(3)
P 		134
(-3)
P 		34
avr 111.2
(-1.9) 		97.9
(-3) 		129.9
(-0.2) 		167.4
(3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 227
1 2 3 4 5 6 range
A 13.5
(-3)
M 		118.2
(2.4)
P 		130.7
(3)
P 		58.2
(-0.7)
P 		115.9
(2.2)
P 		53.9
(-0.9)
P 		117
B 115.6
(-0.7)
P 		206
(2)
P 		37.8
(-3)
P 		96.4
(-1.3)
P 		153.7
(0.4)
P 		240.5
(3)
P 		203
C 83.4
(-0.4)
P 		153
(3)
P 		55.7
(-1.7)
P 		28.6
(-3)
P 		93.6
(0.1)
P 		151.8
(2.9)
P 		124
avr 70.8
(-2.4) 		159.1
(3) 		74.7
(-2.2) 		61.1
(-3) 		121.1
(0.7) 		148.7
(2.4) 		98
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173768_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23243
Sequence Type Exemplar sequence
Representative Public ID CEK078DYR NCBI
Target Description CEK078DYR_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=K08F8.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D71575 /WB_GENE_ID=K08F8.3 /WP=CE03469 /CHR=2 /FEA=Genomic Cluster /DEF=fucosyltransferase; C.elegans cDNA clone yk78d11 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063461(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063461 NCBI Caenorhabditis elegans K08F8.3 (fucosyltransferase) mRNA, complete cds. 11/11 None
K08F8.3.1 ENSEMBL cdna:known chromosome:CEL140:II:8756236:8758550:-1 gene:K08F8.3 11/11 None
K08F8.3.2 ENSEMBL cdna:known chromosome:CEL140:II:8756278:8758642:-1 gene:K08F8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8756243-8758665(-) 100.0 100.0
Public Domain and Genome References
Gene Title fucosyltransferase
Gene Symbol fut-1
Ensembl K08F8.3
Entrez Gene 174400 Entrez gene
SwissProt Q21362 EMBL-EBI
WormBase CE32907 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6486 protein amino acid glycosylation inferred from electronic annotation QuickGO AmiGO
16051 carbohydrate biosynthesis inferred from direct assay QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8417 fucosyltransferase activity inferred from electronic annotation QuickGO AmiGO
46920 alpha(1,3)-fucosyltransferase activity inferred from direct assay QuickGO AmiGO
46920 alpha(1,3)-fucosyltransferase activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91285 Hypothetical protein K08F8.3 [Caenorhabditis elegans] ref|NP_495862.2| K08F8.3 [Caenorhabditis elegans] emb|CAG32977.1| core alpha1,3-fucosyltransferase [Caenorhabditis elegans] 0.0
blast CAE59716 Hypothetical protein CBG03148 [Caenorhabditis briggsae] 0.0
blast AAX26665 SJCHGC04420 protein [Schistosoma japonicum] 3.0E-43
Protein Domains
Database ID Description E-Value
Pfam IPR001503 EMBL-EBI Glycosyl transferase, family 10 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495862.2 1 13-32
K08F8.3.1 1 13-32
K08F8.3.2 1 13-32
Sequence
Target Sequence 
>C. ELEGANS:173768_AT
aagctagagtattttcccaattattatttcaaagcagaactatttctgttagaatttctt
ctgtatttttcttaattgttcttgttccatctattcttcacgggttttattctttaagac
tacatgattgttttcacatttactgatatcggtattgattcttcatttatttattcagtt
gggttttctcaactggtatttaatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGCTAGAGTATTTTCCCAATTATT 680 147 1380 Antisense
ATTTCTGTTAGAATTTCTTCTGTAT 398 17 1421 Antisense
TCTTCTGTATTTTTCTTAATTGTTC 583 617 1436 Antisense
AATTGTTCTTGTTCCATCTATTCTT 302 179 1453 Antisense
TCCATCTATTCTTCACGGGTTTTAT 195 591 1465 Antisense
GGGTTTTATTCTTTAAGACTACATG 403 495 1481 Antisense
AAGACTACATGATTGTTTTCACATT 78 153 1495 Antisense
GTTTTCACATTTACTGATATCGGTA 376 449 1509 Antisense
GATATCGGTATTGATTCTTCATTTA 122 427 1524 Antisense
TTATTTATTCAGTTGGGTTTTCTCA 377 679 1546 Antisense
GGTTTTCTCAACTGGTATTTAATAT 421 505 1561 Antisense
Sequence Source Affymetrix Proprietary Database