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[173751_at] SNAP-25 family

Description C54220_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=Y22F5A.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C54220 /WB_GENE_ID=Y22F5A.3 /WP=CE16604 /CHR=5 /FEA=Genomic Cluster /DEF=SNAP-25 family; C.elegans cDNA clone yk354e6 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 02      4grp_highest_8_8 > 47      4grp_high_8_8 > 20      6grp_highest_nonorm > 12      6grp_total > 24      4grp_total > 20      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2681
1 2 3 4 range
A 2624.6
(0)
P 		2007.6
(-1.3)
P 		1264.8
(-3)
P 		3945.9
(3)
P 		2681
B 2321.8
(-0.9)
P 		2384
(-0.7)
P 		1534.6
(-3)
P 		3746.9
(3)
P 		2212
C 2648.6
(0.2)
P 		2259.5
(-1)
P 		1636.7
(-3)
P 		3546.7
(3)
P 		1910
avr 2531.7
(-0.2) 		2217
(-1) 		1478.7
(-3) 		3746.5
(3) 		2268

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1304
1 2 3 4 5 6 range
A 2445.9
(2.6)
P 		2350.5
(2.2)
P 		2516.3
(3)
P 		1915
(-0)
P 		1411.7
(-2.6)
P 		1332
(-3)
P 		1184
B 2403.5
(3)
P 		1979.8
(0.3)
P 		1591.1
(-2.1)
P 		1565.1
(-2.3)
P 		1446.4
(-3)
P 		1471.7
(-2.8)
P 		957
C 2627.3
(3)
P 		2200.3
(1)
P 		2108.9
(0.6)
P 		1588.2
(-1.8)
P 		1323.3
(-3)
P 		1484.1
(-2.3)
P 		1304
avr 2492.2
(3) 		2176.9
(1.3) 		2072.1
(0.7) 		1689.4
(-1.4) 		1393.8
(-3) 		1429.3
(-2.8) 		1098
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 625
1 2 3 4 range
A 955.6
(3)
P 		435.8
(-2)
P 		330.6
(-3)
P 		577.6
(-0.6)
P 		625
B 740.4
(2.2)
P 		588.8
(0.2)
P 		355.6
(-3)
P 		798.5
(3)
P 		443
C 876.8
(3)
P 		649.6
(-1.4)
P 		570.4
(-3)
P 		598
(-2.5)
P 		306
avr 857.6
(3) 		558.1
(-1.1) 		418.9
(-3) 		658
(0.3) 		439

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 654
1 2 3 4 5 6 range
A 450.9
(-1.5)
P 		836
(3)
P 		548
(-0.4)
P 		485.7
(-1.1)
P 		426
(-1.8)
P 		324.3
(-3)
P 		512
B 836.3
(3)
P 		659
(0.6)
P 		386.4
(-3)
P 		414.5
(-2.6)
P 		587.7
(-0.3)
P 		548.8
(-0.8)
P 		450
C 973.8
(3)
P 		671.4
(0.2)
P 		536.8
(-1)
P 		319.4
(-3)
P 		428.2
(-2)
P 		496.8
(-1.4)
P 		654
avr 753.7
(3) 		722.1
(2.5) 		490.4
(-1.6) 		406.5
(-3) 		480.6
(-1.7) 		456.6
(-2.1) 		347
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173751_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25250
Sequence Type Exemplar sequence
Representative Public ID C54220 NCBI
Target Description C54220_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=Y22F5A.3 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C54220 /WB_GENE_ID=Y22F5A.3 /WP=CE16604 /CHR=5 /FEA=Genomic Cluster /DEF=SNAP-25 family; C.elegans cDNA clone yk354e6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073240(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073240 NCBI Caenorhabditis elegans Resistance to Inhibitors of Cholinesterase family member (ric-4) (ric-4) mRNA, complete cds. 11/11 None
Y22F5A.3 ENSEMBL cdna:known chromosome:CEL140:V:10263713:10272895:-1 gene:Y22F5A.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10263781-10265245(-) 100.0 100.0
Public Domain and Genome References
Gene Title SNAP-25 family
Gene Symbol ric-4
Ensembl Y22F5A.3
Entrez Gene 179427 Entrez gene
SwissProt O62414 EMBL-EBI
WormBase CE32008 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604613_AT similar to Synaptosomal-associated protein 23 (SNAP-23) (Vesicle-membrane fusion protein SNAP-23) (Syndet) cfa
CANINE_2:CFA.18926.1.S1_AT similar to Synaptosomal-associated protein 23 (SNAP-23) (Vesicle-membrane fusion protein SNAP-23) (Syndet) cfa
CANINE_2:CFAAFFX.17344.1.S1_S_AT similar to Synaptosomal-associated protein 23 (SNAP-23) (Vesicle-membrane fusion protein SNAP-23) (Syndet) cfa
HG-U133_PLUS_2:209130_AT synaptosomal-associated protein, 23kDa hs
HG-U133A:209130_AT synaptosomal-associated protein, 23kDa hs
HG-FOCUS:209130_AT synaptosomal-associated protein, 23kDa hs
HG-U133A_2:209130_AT synaptosomal-associated protein, 23kDa hs
HG-U133_PLUS_2:209131_S_AT synaptosomal-associated protein, 23kDa hs
HG-U133A:209131_S_AT synaptosomal-associated protein, 23kDa hs
HG-U133A_2:209131_S_AT synaptosomal-associated protein, 23kDa hs
HU35KSUBB:RC_N25249_AT synaptosomal-associated protein, 23kDa hs
HU35KSUBD:RC_AA342059_S_AT synaptosomal-associated protein, 23kDa hs
HUGENEFL:U55936_AT synaptosomal-associated protein, 23kDa hs
HG-U133_PLUS_2:214544_S_AT synaptosomal-associated protein, 23kDa hs
HG-U133A_2:214544_S_AT synaptosomal-associated protein, 23kDa hs
HG-U133A:214544_S_AT synaptosomal-associated protein, 23kDa hs
U133_X3P:G1374812_3P_A_AT synaptosomal-associated protein, 23kDa hs
U133_X3P:G13277555_3P_AT synaptosomal-associated protein, 23kDa hs
HG-U95AV2:32179_S_AT synaptosomal-associated protein, 23kDa hs
HG-U95AV2:32178_R_AT synaptosomal-associated protein, 23kDa hs
HG-U95B:46749_I_AT synaptosomal-associated protein, 23kDa hs
HG-U95B:42622_AT Synaptosomal-associated protein, 23kDa hs
HG-U95E:88736_AT Synaptosomal-associated protein, 23kDa hs
HU35KSUBB:RC_H02552_AT Synaptosomal-associated protein, 23kDa hs
MU11KSUBA:AB000822_AT synaptosomal-associated protein 23 mm
MU11KSUBA:AB000822_G_AT synaptosomal-associated protein 23 mm
MU11KSUBA:AA125580_AT synaptosomal-associated protein 23 mm
MOE430A:1420896_AT synaptosomal-associated protein 23 mm
MOUSE430A_2:1420896_AT synaptosomal-associated protein 23 mm
MOUSE430_2:1420896_AT synaptosomal-associated protein 23 mm
MOE430A:1420897_AT synaptosomal-associated protein 23 mm
MOUSE430A_2:1420897_AT synaptosomal-associated protein 23 mm
MOUSE430_2:1420897_AT synaptosomal-associated protein 23 mm
MOE430A:1420898_AT synaptosomal-associated protein 23 mm
MOUSE430A_2:1420898_AT synaptosomal-associated protein 23 mm
MOUSE430_2:1420898_AT synaptosomal-associated protein 23 mm
MG-U74AV2:96945_AT synaptosomal-associated protein 23 mm
MU19KSUBB:TC30921_AT Synaptosomal-associated protein 23 (Snap23), mRNA mm
MU19KSUBB:TC30921_G_AT Synaptosomal-associated protein 23 (Snap23), mRNA mm
Protein Similarities
Method ID Description E-Value
blast CAA16322 Hypothetical protein Y22F5A.3 [Caenorhabditis elegans] ref|NP_505641.2| Resistance to Inhibitors of Cholinesterase family member (ric-4) [Caenorhabditis elegans] 1.0E-116
blast CAE64778 Hypothetical protein CBG09569 [Caenorhabditis briggsae] 1.0E-101
blast AAM18191 SNAP25 fusion protein [Loligo pealei] 1.0E-70
Protein Domains
Database ID Description E-Value
Pfam IPR000928 EMBL-EBI SNAP-25 1.8E-25
Pfam IPR000727 EMBL-EBI Target SNARE coiled-coil domain 1.8E-18
Sequence
Target Sequence 
>C. ELEGANS:173751_AT
tgttttcaaaactctctgtgtctcatcttaccattccattttccattgcataatttctca
aaactcccagtatttttctcctactcttggaccagttgtttttttcgattgaaaattcaa
aaattgttattcaatcaacaaattatttttttttaaatcaaaaaaacacccatttccaca
aaaaacttaaaatatagctgaaacttgaaacacctggtagttgtgagtatttacattccc
ccat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTTTTCAAAACTCTCTGTGTCTCA 250 561 727 Antisense
TCAAAACTCTCTGTGTCTCATCTTA 6 631 732 Antisense
TTACCATTCCATTTTCCATTGCATA 262 683 754 Antisense
TCCATTTTCCATTGCATAATTTCTC 509 591 761 Antisense
GCATAATTTCTCAAAACTCCCAGTA 329 307 774 Antisense
TCAAAACTCCCAGTATTTTTCTCCT 569 629 784 Antisense
TTCTCCTACTCTTGGACCAGTTGTT 421 693 802 Antisense
TGGACCAGTTGTTTTTTTCGATTGA 288 547 814 Antisense
ATAGCTGAAACTTGAAACACCTGGT 28 23 920 Antisense
GAAACACCTGGTAGTTGTGAGTATT 149 357 933 Antisense
GTTGTGAGTATTTACATTCCCCCAT 647 435 946 Antisense
Sequence Source Affymetrix Proprietary Database