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[173750_at]

Description AV198655_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C29E4.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV198655 /WB_GENE_ID=C29E4.5 /WP=CE25794 /CHR=3 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk191g10 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 53      6grp_high1_10_10 > 35      4grp_highest_8_8 > 21      4grp_high_8_8 > 15      6grp_highest_nonorm > 74      6grp_total > 36      4grp_total > 16      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 671
1 2 3 4 range
A 717.7
(-3)
P 		895.7
(-1.4)
P 		984.2
(-0.6)
P 		1388.3
(3)
P 		671
B 781.4
(-3)
P 		786.2
(-2.9)
P 		832.2
(-2.2)
P 		1148.6
(3)
P 		367
C 768.6
(-2.6)
P 		755.3
(-3)
P 		886.4
(0.6)
P 		975.4
(3)
P 		220
avr 755.9
(-3) 		812.4
(-2.2) 		900.9
(-0.9) 		1170.8
(3) 		415

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 600
1 2 3 4 5 6 range
A 871.3
(2.7)
P 		668.2
(0.2)
P 		679.3
(0.3)
P 		420.9
(-3)
P 		891.6
(3)
P 		711.1
(0.7)
P 		471
B 800.8
(-0.2)
P 		1020.7
(3)
P 		673.3
(-2.1)
P 		1017.7
(3)
P 		614.1
(-3)
P 		802.3
(-0.2)
P 		407
C 588.6
(-1.6)
P 		822.3
(3)
P 		520.6
(-3)
P 		611.5
(-1.2)
P 		528.1
(-2.9)
P 		668
(-0.1)
P 		302
avr 753.6
(0.6) 		837.1
(3) 		624.4
(-3) 		683.4
(-1.3) 		677.9
(-1.5) 		727.1
(-0.1) 		213
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 145
1 2 3 4 range
A 261.3
(3)
P 		194.4
(-3)
P 		257.3
(2.6)
P 		203.2
(-2.2)
P 		67
B 249.2
(3)
P 		194.2
(-2.9)
P 		192.8
(-3)
P 		244.8
(2.5)
P 		56
C 254.4
(0.7)
P 		217.2
(-0.8)
P 		309
(3)
P 		164.5
(-3)
P 		145
avr 255
(3) 		201.9
(-3) 		253
(2.8) 		204.2
(-2.7) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 233
1 2 3 4 5 6 range
A 160.6
(-1)
P 		237.6
(1.8)
P 		147.9
(-1.5)
P 		106.7
(-3)
P 		269.1
(3)
P 		173.1
(-0.5)
P 		162
B 278.6
(0.9)
P 		339.7
(3)
P 		163.5
(-3)
P 		269.6
(0.6)
P 		249.5
(-0.1)
P 		299.2
(1.6)
P 		176
C 218.2
(1.5)
P 		250.9
(3)
P 		132.5
(-2.6)
P 		123
(-3)
P 		170.9
(-0.8)
P 		223.6
(1.7)
P 		128
avr 219.1
(0.3) 		276.1
(3) 		148
(-3) 		166.4
(-2.1) 		229.8
(0.8) 		232
(0.9) 		128
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173750_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24191
Sequence Type Exemplar sequence
Representative Public ID AV198655 NCBI
Target Description AV198655_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C29E4.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV198655 /WB_GENE_ID=C29E4.5 /WP=CE25794 /CHR=3 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk191g10 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026017(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026017 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-250) (tag-250) mRNA, complete cds. 11/11 None
C29E4.5 ENSEMBL cdna:known chromosome:CEL140:III:7917918:7924791:-1 gene:C29E4.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176113 2 1 NM_001026017 173750_at (11), 175951_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7917986-7924790(-) 100.0 100.0
Public Domain and Genome References
Ensembl C29E4.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA27964 Temporarily assigned gene name protein 250, isoform a [Caenorhabditis elegans] ref|NP_001021188.1| Temporarily Assigned Gene name family member (tag-250) [Caenorhabditis elegans] sp|P34344|TG250_CAEEL Hypothetical protein tag-250 0.0
blast CAE66904 Hypothetical protein CBG12287 [Caenorhabditis briggsae] 0.0
blast AAY44021 Temporarily assigned gene name protein 250, isoform b [Caenorhabditis elegans] ref|NP_001021189.1| Temporarily Assigned Gene name family member (tag-250) [Caenorhabditis elegans] 4.0E-57
Protein Domains
Database ID Description E-Value
Pfam IPR008191 EMBL-EBI Maternal tudor protein 2.9E-11
Pfam IPR008191 EMBL-EBI Maternal tudor protein 4.0E-17
Sequence
Target Sequence 
>C. ELEGANS:173750_AT
gaatattttctgatccgtcttcattacaaagcttctaatcaattttgtgctttatttttt
tctaatttttgaatattgtatattttcataattcctcagttttgccgattagtagtcttt
ctctgaaaattcaaaaatatttgctcagttttccacttttctcttcagtttttattct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATATTTTCTGATCCGTCTTCATT 146 327 1806 Antisense
TCTGATCCGTCTTCATTACAAAGCT 578 603 1814 Antisense
AGCTTCTAATCAATTTTGTGCTTTA 452 79 1835 Antisense
TATTGTATATTTTCATAATTCCTCA 703 665 1879 Antisense
TTCATAATTCCTCAGTTTTGCCGAT 2 687 1890 Antisense
AATTCCTCAGTTTTGCCGATTAGTA 488 177 1895 Antisense
GTTTTGCCGATTAGTAGTCTTTCTC 173 449 1904 Antisense
GCCGATTAGTAGTCTTTCTCTGAAA 239 283 1909 Antisense
ATATTTGCTCAGTTTTCCACTTTTC 672 19 1942 Antisense
TTCCACTTTTCTCTTCAGTTTTTAT 671 699 1956 Antisense
CACTTTTCTCTTCAGTTTTTATTCT 695 195 1959 Antisense
Sequence Source Affymetrix Proprietary Database