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[173743_at] elongation factor

Description CEC3362 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=H19N07.1 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C09336 /WB_GENE_ID=H19N07.1 /WP=CE16181 /CHR=5 /FEA=Genomic Cluster /DEF=elongation factor; C.elegans cDNA clone yk167a5 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 17      4grp_highest_8_8 > 18      4grp_high_8_8 > 63      6grp_highest_nonorm > 21      6grp_total > 06      4grp_total > 56      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2261
1 2 3 4 range
A 1293.6
(3)
P 		603.8
(-3)
P 		1115
(1.4)
P 		855.9
(-0.8)
P 		690
B 703.9
(-3)
P 		1983.8
(3)
P 		1939.3
(2.8)
P 		1275.8
(-0.3)
P 		1280
C 1826
(0.6)
P 		1151.5
(-1.2)
P 		2741.4
(3)
P 		480.7
(-3)
P 		2261
avr 1274.5
(-0.7) 		1246.4
(-0.9) 		1931.9
(3) 		870.8
(-3) 		1061

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1548
1 2 3 4 5 6 range
A 1232
(0.9)
P 		1414.3
(2.2)
P 		1535.2
(3)
P 		905.2
(-1.4)
P 		876.7
(-1.6)
P 		669.2
(-3)
P 		866
B 2145.5
(3)
P 		1110.3
(-2.2)
P 		1863.2
(1.6)
P 		958
(-3)
P 		1262.7
(-1.5)
P 		956.2
(-3)
P 		1189
C 1717.7
(3)
P 		791.6
(-2)
P 		766.2
(-2.1)
P 		597.7
(-3)
P 		1523
(2)
P 		1195.7
(0.2)
P 		1120
avr 1698.4
(3) 		1105.4
(-1.1) 		1388.2
(0.9) 		820.3
(-3) 		1220.8
(-0.3) 		940.4
(-2.2) 		878
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 874
1 2 3 4 range
A 471
(3)
P 		131.1
(-2.9)
P 		291.5
(-0.1)
P 		125.3
(-3)
P 		346
B 224.5
(-3)
P 		489.9
(3)
P 		449.4
(2.1)
P 		271.9
(-1.9)
P 		265
C 604.5
(0.6)
P 		331.1
(-1.3)
P 		955.5
(3)
P 		81.1
(-3)
P 		874
avr 433.3
(1) 		317.4
(-0.7) 		565.5
(3) 		159.4
(-3) 		406

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 626
1 2 3 4 5 6 range
A 227.1
(-1.9)
P 		503
(3)
P 		334.3
(0)
P 		229.6
(-1.8)
P 		264.5
(-1.2)
P 		162.9
(-3)
P 		340
B 746.5
(3)
P 		369.6
(-1.6)
P 		452.4
(-0.6)
P 		253.7
(-3)
P 		513
(0.2)
P 		356.6
(-1.7)
P 		493
C 636.7
(3)
P 		241.5
(-1.6)
P 		195
(-2.1)
P 		120.2
(-3)
P 		492.8
(1.3)
P 		400.3
(0.3)
P 		517
avr 536.8
(3) 		371.4
(0) 		327.2
(-0.7) 		201.2
(-3) 		423.4
(1) 		306.6
(-1.1) 		336
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173743_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25392
Sequence Type Exemplar sequence
Representative Public ID CEC3362 NCBI
Target Description CEC3362 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=H19N07.1 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C09336 /WB_GENE_ID=H19N07.1 /WP=CE16181 /CHR=5 /FEA=Genomic Cluster /DEF=elongation factor; C.elegans cDNA clone yk167a5 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073423(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073423 NCBI Caenorhabditis elegans H19N07.1 (H19N07.1) mRNA, complete cds. 11/11 None
H19N07.1.1 ENSEMBL cdna:known chromosome:CEL140:V:11112651:11115691:-1 gene:H19N07.1 11/11 A 
H19N07.1.2 ENSEMBL cdna:known chromosome:CEL140:V:11112707:11115679:-1 gene:H19N07.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000011320 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11112761-11115659(-) 100.0 100.0
Public Domain and Genome References
Gene Title elongation factor
Ensembl H19N07.1
Entrez Gene 179537 Entrez gene
SwissProt O45622 EMBL-EBI
WormBase CE32460 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256119_AT EF-1-alpha-related GTP-binding protein, putative at
CANINE:1600894_AT similar to G1 to S phase transition protein 1 homolog (GTP-binding protein GST1-HS) cfa
CANINE_2:CFA.20162.1.S1_S_AT similar to G1 to S phase transition protein 1 homolog (GTP-binding protein GST1-HS) cfa
DROSOPHILA_2:1640557_AT Ef1like factor dm
DROSGENOME1:143928_AT Ef1like factor dm
CHICKEN:GGA.18312.1.A1_AT G1 to S phase transition 1 gga
CHICKEN:GGA.6351.1.S1_AT G1 to S phase transition 1 gga
CHICKEN:GGA.9336.1.S1_AT G1 to S phase transition 1 gga
CHICKEN:GGA.9336.2.S1_A_AT G1 to S phase transition 1 gga
CHICKEN:GGAAFFX.11886.1.S1_S_AT G1 to S phase transition 1 gga
HG-U133_PLUS_2:215438_X_AT G1 to S phase transition 1 hs
HG-U133A_2:215438_X_AT G1 to S phase transition 1 hs
HG-U133A:215438_X_AT G1 to S phase transition 1 hs
PLASMODIUM_ANOPHELES:201912_S_AT G1 to S phase transition 1 hs
HG-U133_PLUS_2:201912_S_AT G1 to S phase transition 1 hs
HG-U133A:201912_S_AT G1 to S phase transition 1 hs
HG-FOCUS:201912_S_AT G1 to S phase transition 1 hs
HG-U133A_2:201912_S_AT G1 to S phase transition 1 hs
HUGENEFL:X17644_S_AT G1 to S phase transition 1 hs
U133_X3P:G4504166_3P_A_AT G1 to S phase transition 1 hs
U133_X3P:HS.2707.2.S1_3P_X_AT G1 to S phase transition 1 hs
U133_X3P:HS.2707.2.S1_3P_A_AT G1 to S phase transition 1 hs
U133_X3P:215438_3P_X_AT G1 to S phase transition 1 hs
HG-U95AV2:33932_AT G1 to S phase transition 1 hs
HG-U95B:58796_AT G1 to S phase transition 1 hs
HG-U133_PLUS_2:217595_AT G1 to S phase transition 1 hs
HG-U133A_2:217595_AT G1 to S phase transition 1 hs
HG-U133A:217595_AT G1 to S phase transition 1 hs
HU35KSUBA:AA039996_AT G1 to S phase transition 1 hs
HU35KSUBA:RC_AA112361_AT G1 to S phase transition 1 hs
HU35KSUBB:RC_N59481_AT G1 to S phase transition 1 hs
U133_X3P:HS.32204.0.A1_3P_AT G1 to S phase transition 1 hs
U133_X3P:HS.237849.0.A1_3P_AT G1 to S phase transition 1 hs
U133_X3P:HS.10592.0.A1_3P_AT G1 to S phase transition 1 hs
HG-U133_PLUS_2:225276_AT G1 to S phase transition 1 hs
HG-U133B:225276_AT G1 to S phase transition 1 hs
HG-U133_PLUS_2:234975_AT G1 to S phase transition 1 hs
HG-U133B:234975_AT G1 to S phase transition 1 hs
HG-U95C:58486_AT G1 to S phase transition 1 hs
HG-U95C:64091_AT G1 to S phase transition 1 hs
HG-U95D:80058_AT G1 to S phase transition 1 hs
HG-U133_PLUS_2:240452_AT G1 to S phase transition 1 hs
HG-U133B:240452_AT G1 to S phase transition 1 hs
HG-U95B:42688_AT G1 to S phase transition 1 hs
HG-U95E:81055_AT G1 to S phase transition 1 hs
U133_X3P:HS.112264.0.A1_3P_AT G1 to S phase transition 1 hs
HU35KSUBB:RC_R62452_AT G1 to S phase transition 1 hs
HU35KSUBD:RC_AA620636_AT G1 to S phase transition 1 hs
MU11KSUBA:AA409254_RC_AT G1 to S phase transition 1 mm
MU11KSUBB:MSA.40366.0_S_AT G1 to S phase transition 1 mm
MU11KSUBB:MSA.28090.0_S_AT G1 to S phase transition 1 mm
MU11KSUBB:MSA.40360.0_F_AT G1 to S phase transition 1 mm
MG-U74AV2:93727_AT G1 to S phase transition 1 mm
MG-U74BV2:110389_AT G1 to S phase transition 1 mm
MG-U74BV2:110390_G_AT G1 to S phase transition 1 mm
MG-U74BV2:109185_AT G1 to S phase transition 1 mm
MG-U74CV2:168708_I_AT G1 to S phase transition 1 mm
MG-U74CV2:168329_AT G1 to S phase transition 1 mm
MOE430A:1426736_AT G1 to S phase transition 1 mm
MOUSE430A_2:1426736_AT G1 to S phase transition 1 mm
MOUSE430_2:1426736_AT G1 to S phase transition 1 mm
MOE430A:1426737_AT G1 to S phase transition 1 mm
MOUSE430A_2:1426737_AT G1 to S phase transition 1 mm
MOUSE430_2:1426737_AT G1 to S phase transition 1 mm
MOE430A:1452168_X_AT G1 to S phase transition 1 mm
MOUSE430A_2:1452168_X_AT G1 to S phase transition 1 mm
MOUSE430_2:1452168_X_AT G1 to S phase transition 1 mm
MOE430B:1435000_AT G1 to S phase transition 1 mm
MOUSE430_2:1435000_AT G1 to S phase transition 1 mm
MOE430B:1455173_AT G1 to S phase transition 1 mm
MOUSE430_2:1455173_AT G1 to S phase transition 1 mm
MOE430B:1446550_AT G1 to S phase transition 1 mm
MOUSE430_2:1446550_AT G1 to S phase transition 1 mm
MU11KSUBB:MSA.26940.0_AT G1 to S phase transition 1 (Gspt1), mRNA mm
MU19KSUBA:TC24394_AT G1 to S phase transition 1 (Gspt1), mRNA mm
MU19KSUBA:TC25746_AT G1 to S phase transition 1 (Gspt1), mRNA mm
MU19KSUBB:TC32557_AT G1 to S phase transition 1 (Gspt1), mRNA mm
MU19KSUBC:TC35109_AT G1 to S phase transition 1 (Gspt1), mRNA mm
MU19KSUBC:TC40354_AT G1 to S phase transition 1 (Gspt1), mRNA mm
MU19KSUBC:TC41571_AT G1 to S phase transition 1 (Gspt1), mRNA mm
RG-U34C:RC_AI229595_AT G1 to S phase transition 1 rn
RAE230A:1371585_AT G1 to S phase transition 1 rn
RAT230_2:1371585_AT G1 to S phase transition 1 rn
RAE230B:1394732_AT G1 to S phase transition 1 rn
RAT230_2:1394732_AT G1 to S phase transition 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07395 Hypothetical protein H19N07.1 [Caenorhabditis elegans] ref|NP_505824.2| H19N07.1 [Caenorhabditis elegans] 0.0
blast CAE75167 Hypothetical protein CBG23104 [Caenorhabditis briggsae] 0.0
blast AAH77825 Gspt2-prov protein [Xenopus laevis] 1.0E-166
Protein Domains
Database ID Description E-Value
Pfam IPR004161 EMBL-EBI Elongation factor Tu, domain 2 1.3E-13
Pfam IPR000795 EMBL-EBI Protein synthesis factor, GTP-binding 4.1E-86
Pfam IPR004160 EMBL-EBI Elongation factor Tu, C-terminal 1.2E-32
Sequence
Target Sequence 
>C. ELEGANS:173743_AT
aaagttgtcgagtaatttcattgataaattagtcatttctattctaaaaattgttttttt
ttctgatttcagccggtccgttttctgtccctcccacatgtcgcttttcactatttttct
tttagattccacatgtttccattgggaagattgttgaatctaatgttaccaatcctattt
ctaatagaaattacctgaattcattttcccttccactatccataaagcaaaattctattc
gt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGTTGTCGAGTAATTTCATTGAT 123 123 1721 Antisense
GATAAATTAGTCATTTCTATTCTAA 409 423 1743 Antisense
TTTTTTCTGATTTCAGCCGGTCCGT 48 667 1777 Antisense
CCACATGTCGCTTTTCACTATTTTT 331 273 1814 Antisense
TCACTATTTTTCTTTTAGATTCCAC 462 627 1828 Antisense
TTAGATTCCACATGTTTCCATTGGG 648 627 1842 Antisense
GAATCTAATGTTACCAATCCTATTT 640 327 1876 Antisense
AATGTTACCAATCCTATTTCTAATA 676 163 1882 Antisense
GAAATTACCTGAATTCATTTTCCCT 141 363 1907 Antisense
TTTCCCTTCCACTATCCATAAAGCA 168 671 1925 Antisense
ATCCATAAAGCAAAATTCTATTCGT 548 39 1938 Antisense
Sequence Source Affymetrix Proprietary Database