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[173730_at]

Description AV183471_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=M05D6.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV183471 /WB_GENE_ID=M05D6.5 /WP=CE03503 /CHR=2 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk652a7 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 85      6grp_high1_10_10 > 68      4grp_highest_8_8 > 13      4grp_high_8_8 > 31      6grp_highest_nonorm > 84      6grp_total > 69      4grp_total > 24      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1330
1 2 3 4 range
A 864.3
(-3)
P 		1042.7
(0.7)
P 		1157
(3)
P 		1061.5
(1)
P 		293
B 562.4
(-3)
P 		778.4
(-2)
P 		1183.9
(-0.2)
P 		1892.2
(3)
P 		1330
C 620.7
(-3)
P 		1415.4
(2)
P 		1572.2
(3)
P 		1525.3
(2.7)
P 		952
avr 682.5
(-3) 		1078.8
(-0.1) 		1304.4
(1.6) 		1493
(3) 		811

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 706
1 2 3 4 5 6 range
A 537.5
(0.4)
P 		607.4
(1.4)
P 		638.4
(1.8)
P 		279.8
(-3)
P 		728.3
(3)
P 		679.1
(2.3)
P 		449
B 675.7
(-0.7)
P 		779.7
(1.2)
P 		753.4
(0.8)
P 		572.3
(-2.6)
P 		552.4
(-3)
P 		873.6
(3)
P 		321
C 807.7
(1.3)
P 		359.4
(-3)
P 		514.5
(-1.5)
P 		391.5
(-2.7)
P 		616.4
(-0.5)
P 		986.2
(3)
P 		627
avr 673.6
(0.6) 		582.2
(-0.7) 		635.4
(0.1) 		414.5
(-3) 		632.4
(0) 		846.3
(3) 		432
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 393
1 2 3 4 range
A 314.7
(3)
P 		226.3
(-0.3)
P 		302.5
(2.5)
P 		155.4
(-3)
P 		159
B 179.3
(-3)
P 		192.3
(-2.7)
P 		274.3
(-0.5)
P 		403.2
(3)
P 		224
C 205.5
(-3)
P 		406.9
(0.5)
P 		548
(3)
P 		257.2
(-2.1)
P 		343
avr 233.2
(-3) 		275.2
(-1.2) 		374.9
(3) 		271.9
(-1.4) 		142

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 259
1 2 3 4 5 6 range
A 99.1
(-1.9)
P 		216
(2.8)
P 		139
(-0.3)
P 		71
(-3)
P 		219.8
(3)
P 		165.4
(0.8)
P 		149
B 235.1
(-0.1)
P 		259.5
(0.7)
P 		182.9
(-1.9)
P 		151.6
(-3)
P 		224.4
(-0.5)
P 		325.8
(3)
P 		174
C 299.4
(2.3)
P 		109.7
(-2.3)
P 		130.9
(-1.8)
P 		78.7
(-3)
P 		199.5
(-0.1)
P 		330.1
(3)
P 		251
avr 211.2
(0.8) 		195.1
(0.3) 		150.9
(-1.3) 		100.4
(-3) 		214.6
(1) 		273.8
(3) 		173
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173730_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23395
Sequence Type Exemplar sequence
Representative Public ID AV183471 NCBI
Target Description AV183471_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=M05D6.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV183471 /WB_GENE_ID=M05D6.5 /WP=CE03503 /CHR=2 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk652a7 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063390(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063390 NCBI Caenorhabditis elegans M05D6.5 (M05D6.5) mRNA, complete cds. 11/11 None
M05D6.5.1 ENSEMBL cdna:known chromosome:CEL140:II:8480420:8482138:-1 gene:M05D6.5 11/11 None
M05D6.5.2 ENSEMBL cdna:known chromosome:CEL140:II:8480435:8482140:-1 gene:M05D6.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8480474-8482114(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl M05D6.5
Entrez Gene 174357 Entrez gene
SwissProt Q21524 EMBL-EBI
WormBase CE03503 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91414 Hypothetical protein M05D6.5 [Caenorhabditis elegans] ref|NP_495791.1| M05D6.5 [Caenorhabditis elegans] 8.0E-70
blast CAE57632 Hypothetical protein CBG00617 [Caenorhabditis briggsae] 2.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR007667 EMBL-EBI Hypoxia induced protein conserved region 6.9E-30
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495791.1 2 71-93,113-135
M05D6.5.1 2 71-93,113-135
M05D6.5.2 2 71-93,113-135
Sequence
Target Sequence 
>C. ELEGANS:173730_AT
ataaattgtatactttctctcttttccaattttcttatgattagtctctttttatttgct
ttcatctcttatcatcaccgactagaactttccttccgtgatttgaggtttttttttgga
gttttccaaaaaatgtgctttcaaatattttgaagcccggg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATAAATTGTATACTTTCTCTCTTTT 166 25 446 Antisense
TTTCCAATTTTCTTATGATTAGTCT 541 669 468 Antisense
ATTTTCTTATGATTAGTCTCTTTTT 262 15 474 Antisense
GATTAGTCTCTTTTTATTTGCTTTC 421 429 484 Antisense
GTCTCTTTTTATTTGCTTTCATCTC 201 471 489 Antisense
TCATCTCTTATCATCACCGACTAGA 697 619 507 Antisense
TCATCACCGACTAGAACTTTCCTTC 275 619 517 Antisense
ACTAGAACTTTCCTTCCGTGATTTG 606 97 526 Antisense
GAACTTTCCTTCCGTGATTTGAGGT 174 351 530 Antisense
TTTCCAAAAAATGTGCTTTCAAATA 102 669 568 Antisense
GCTTTCAAATATTTTGAAGCCCGGG 12 305 582 Antisense
Sequence Source Affymetrix Proprietary Database