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[173725_s_at]

Description AV193083 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F41H10.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV193083 /WB_GENE_ID=F41H10.8 /WP=CE10286 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk613b7 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 51      4grp_highest_8_8 > 10      4grp_high_8_8 > 62      6grp_highest_nonorm > 17      6grp_total > 51      4grp_total > 57      6grp_highest_sub > 03_04      6grp_cgc > 83      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6506
1 2 3 4 range
A 5399.5
(-0.9)
P 		7017.2
(0.7)
P 		9449.5
(3)
P 		3226.7
(-3)
P 		6223
B 5491.1
(-0.7)
P 		8702.4
(2.3)
P 		9491.5
(3)
P 		2985.6
(-3)
P 		6506
C 4350.6
(-3)
P 		7879.5
(1.6)
P 		8964.2
(3)
P 		4396.8
(-2.9)
P 		4614
avr 5080.4
(-1.4) 		7866.4
(1.5) 		9301.7
(3) 		3536.4
(-3) 		5765

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10820
1 2 3 4 5 6 range
A 11122.1
(-0.8)
P 		11477.1
(-0.5)
P 		17355.2
(3)
P 		12808.1
(0.3)
P 		10824.5
(-0.9)
P 		7394
(-3)
P 		9961
B 9574.5
(-0.3)
P 		11493.1
(1.4)
P 		12592.8
(2.3)
P 		13349.6
(3)
P 		10142.9
(0.2)
P 		6535.4
(-3)
P 		6814
C 8632.5
(-1.7)
P 		11705.4
(1.3)
P 		13369.4
(2.9)
P 		13437.7
(3)
P 		9253.5
(-1.1)
P 		7251.5
(-3)
P 		6186
avr 9776.4
(-0.8) 		11558.5
(0.7) 		14439.1
(3) 		13198.5
(2) 		10073.6
(-0.5) 		7060.3
(-3) 		7379
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2652
1 2 3 4 range
A 1966
(1.5)
P 		1523.2
(0.2)
P 		2470.3
(3)
P 		472.3
(-3)
P 		1998
B 1751.1
(1.3)
P 		2149.3
(2.8)
P 		2199.4
(3)
P 		636.2
(-3)
P 		1563
C 1440.2
(-1.2)
P 		2265.4
(0.8)
P 		3124.3
(3)
P 		741.4
(-3)
P 		2383
avr 1719.1
(0.3) 		1979.3
(1.1) 		2598
(3) 		616.6
(-3) 		1981

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2321
1 2 3 4 5 6 range
A 2050.3
(-2.3)
P 		4081.8
(3)
P 		3779.6
(2.2)
P 		3248.4
(0.8)
P 		3266.4
(0.9)
P 		1800.2
(-3)
P 		2282
B 3331.4
(0.2)
P 		3825.6
(1.9)
P 		3057.9
(-0.8)
P 		3535.8
(0.9)
P 		4120.9
(3)
P 		2437
(-3)
P 		1684
C 3199.7
(1)
P 		3571.7
(3)
P 		3402.7
(2.1)
P 		2702.3
(-1.6)
P 		2994.3
(-0)
P 		2427.5
(-3)
P 		1144
avr 2860.5
(-0.6) 		3826.4
(3) 		3413.4
(1.5) 		3162.2
(0.5) 		3460.5
(1.6) 		2221.6
(-3) 		1605
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173725_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25066
Sequence Type Exemplar sequence
Representative Public ID AV193083 NCBI
Target Description AV193083 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F41H10.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV193083 /WB_GENE_ID=F41H10.8 /WP=CE10286 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk613b7 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068396(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068396 NCBI Caenorhabditis elegans fatty acid ELOngation family member (elo-6) (elo-6) mRNA, complete cds. 11/11 None
SNAP00000003912 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5386713:5388542:-1 11/11 None
GENEFINDER00000003920 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5386713:5388542:-1 11/11 None
F41H10.8 ENSEMBL cdna:known chromosome:CEL140:IV:5386618:5388548:-1 gene:F41H10.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5386719-5388549(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol elo-6
Ensembl F41H10.8
Entrez Gene 177321 Entrez gene
SwissProt Q20303 EMBL-EBI
WormBase CE10286 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29807 Fatty acid elongation protein 6 [Caenorhabditis elegans] ref|NP_500797.1| fatty acid ELOngation family member (elo-6) [Caenorhabditis elegans] 1.0E-153
blast CAE72716 Hypothetical protein CBG19953 [Caenorhabditis briggsae] 1.0E-138
blast CAA92958 Hypothetical protein F56H11.4 [Caenorhabditis elegans] ref|NP_501689.1| fatty acid ELOngation family member (elo-1) [Caenorhabditis elegans] gb|AAF70462.1| long chain polyunsaturated fatty acid elongation enzyme [Caenorhabditis elegans] 2.0E-54
Protein Domains
Database ID Description E-Value
Pfam IPR002076 EMBL-EBI GNS1/SUR4 membrane protein 9.0E-41
Pfam IPR002076 EMBL-EBI GNS1/SUR4 membrane protein 1.6E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500797.1 7 31-50,63-85,110-132,139-156,161-183,203-225,230-252
F41H10.8 7 31-50,63-85,110-132,139-156,161-183,203-225,230-252
SNAP00000003912 7 31-50,63-85,110-132,139-156,161-183,203-225,230-252
GENEFINDER00000003920 7 31-50,63-85,110-132,139-156,161-183,203-225,230-252
Sequence
Target Sequence 
>C. ELEGANS:173725_S_AT
ctggaatgcgggtcttgctattttctcaactctcggatcacttgccactacatttggact
tctccacgagttcttcagccgtggatttttcgaatcttacattcacatcggagactttta
taatggactttctggaatgttcacatggcttttcgttctctcaaaagttgctgaattcgg
agatacactttttattattcttcgtaaaaagccattgatgttccttcattggtatcatca
tgtgcttacaatgaattatgcttttatgtcatttgaagctaatttgggatttaatacttg
gattacatggatgaatttctcagttcactcaattatgtatggatattatatgcttcgttc
ttttggtgtcaaggttccagcatggattgccaagaatattacaacaatgcaaattcttca
attcgttattactcatttcattcttttccacgttggatatttggcagttactggacaatc
tgttgactcaactccaggatattattggttctgccttctcatggaaatctcttatgtcgt
tctgttcggaaacttctact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGGAATGCGGGTCTTGCTATTTTC 214 241 208 Antisense
TGCTATTTTCTCAACTCTCGGATCA 92 581 223 Antisense
GGATCACTTGCCACTACATTTGGAC 285 511 242 Antisense
TTTGGACTTCTCCACGAGTTCTTCA 335 665 260 Antisense
GAGTTCTTCAGCCGTGGATTTTTCG 702 397 275 Antisense
AATGTTCACATGGCTTTTCGTTCTC 216 165 343 Antisense
TTATATGCTTCGTTCTTTTGGTGTC 542 677 553 Antisense
TTACTCATTTCATTCTTTTCCACGT 346 683 636 Antisense
TATTATTGGTTCTGCCTTCTCATGG 117 663 707 Antisense
GGAAATCTCTTATGTCGTTCTGTTC 299 527 730 Antisense
GTCGTTCTGTTCGGAAACTTCTACT 528 471 743 Antisense
Sequence Source Affymetrix Proprietary Database