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[173712_s_at]

Description AV202376 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T23B12.7 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV202376 /WB_GENE_ID=T23B12.7 /WP=CE14038 /CHR=5 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk549f7 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 86      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 93      6grp_total > 76      4grp_total > 03      6grp_highest_sub > In      6grp_cgc > 58      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 349
1 2 3 4 range
A 278.2
(-1.2)
P 		213
(-2.4)
P 		178.7
(-3)
P 		510.2
(3)
P 		332
B 213.1
(-2.4)
P 		198.2
(-2.7)
P 		184
(-3)
P 		473
(3)
P 		289
C 222.6
(-2.1)
P 		172.8
(-3)
P 		235.3
(-1.9)
P 		522.1
(3)
P 		349
avr 238
(-2.2) 		194.7
(-3) 		199.3
(-2.9) 		501.8
(3) 		307

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 717
1 2 3 4 5 6 range
A 204.1
(-3)
P 		242.6
(-2.5)
P 		442.5
(0.3)
P 		357.4
(-0.9)
P 		515.3
(1.3)
P 		642
(3)
P 		438
B 306.4
(-3)
P 		309.5
(-3)
P 		470
(-1.2)
P 		406.4
(-1.9)
P 		492.8
(-0.9)
P 		844.3
(3)
P 		538
C 220.5
(-3)
P 		376.4
(-1.7)
P 		237.9
(-2.9)
P 		498.3
(-0.6)
P 		335.9
(-2)
P 		920.6
(3)
P 		700
avr 243.7
(-3) 		309.5
(-2.3) 		383.5
(-1.5) 		420.7
(-1.1) 		448
(-0.8) 		802.3
(3) 		559
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 101.3
(3)
P 		46.2
(-3)
P 		46.7
(-2.9)
P 		74.7
(0.1)
P 		55
B 68
(-0.4)
P 		49
(-2.3)
P 		42.6
(-3)
P 		100.8
(3)
P 		58
C 73.7
(0.8)
P 		49.7
(-3)
P 		82
(2.1)
P 		88
(3)
P 		38
avr 81
(2) 		48.3
(-3) 		57.1
(-1.7) 		87.8
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 277
1 2 3 4 5 6 range
A 37.6
(-3)
P 		86.3
(-0.5)
P 		96.4
(-0)
P 		90.7
(-0.3)
P 		155.5
(3)
P 		156.3
(3)
P 		119
B 106.6
(-2.9)
P 		103
(-3)
P 		114.1
(-2.7)
P 		107.6
(-2.9)
P 		200.2
(-0.2)
P 		314.8
(3)
P 		212
C 81.7
(-2.5)
P 		114.9
(-1.7)
P 		60.5
(-3)
P 		100.2
(-2)
P 		108.7
(-1.8)
P 		308.2
(3)
P 		248
avr 75.3
(-3) 		101.4
(-2.2) 		90.3
(-2.5) 		99.5
(-2.2) 		154.8
(-0.4) 		259.8
(3) 		185
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173712_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25195
Sequence Type Exemplar sequence
Representative Public ID AV202376 NCBI
Target Description AV202376 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T23B12.7 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV202376 /WB_GENE_ID=T23B12.7 /WP=CE14038 /CHR=5 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk549f7 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072777(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072777 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-22) (dnj-22) mRNA, complete cds. 11/11 None
SNAP00000042000 ENSEMBL cdna:SNAP chromosome:CEL140:V:8463761:8464696:-1 11/11 None
GENEFINDER00000042013 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8463761:8464696:-1 11/11 None
T23B12.7 ENSEMBL cdna:known chromosome:CEL140:V:8463685:8464696:-1 gene:T23B12.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8463696-8464698(-) 96.97 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dnj-22
Ensembl T23B12.7
Entrez Gene 179227 Entrez gene
SwissProt O17002 EMBL-EBI
WormBase CE14038 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:249844_AT DNAJ heat shock N-terminal domain-containing protein at
CANINE:1605595_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 17 cfa
CANINE_2:CFA.17995.1.S1_S_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 17 cfa
DROSOPHILA_2:1625165_AT dm
DROSGENOME1:142900_AT dm
CHICKEN:GGA.12264.2.S1_A_AT DnaJ (Hsp40) homolog, subfamily C, member 17 gga
CHICKEN:GGAAFFX.2749.1.S1_AT DnaJ (Hsp40) homolog, subfamily C, member 17 gga
HG-U133_PLUS_2:219861_AT DnaJ (Hsp40) homolog, subfamily C, member 17 hs
HG-U133A:219861_AT DnaJ (Hsp40) homolog, subfamily C, member 17 hs
HG-U133A_2:219861_AT DnaJ (Hsp40) homolog, subfamily C, member 17 hs
U133_X3P:G8922562_3P_AT DnaJ (Hsp40) homolog, subfamily C, member 17 hs
MOE430A:1420165_S_AT DnaJ (Hsp40) homolog, subfamily C, member 17 mm
MOUSE430_2:1420165_S_AT DnaJ (Hsp40) homolog, subfamily C, member 17 mm
MOUSE430A_2:1420165_S_AT DnaJ (Hsp40) homolog, subfamily C, member 17 mm
MU11KSUBA:N28111_RC_AT DnaJ (Hsp40) homolog, subfamily C, member 17 mm
MG-U74AV2:160730_AT DnaJ (Hsp40) homolog, subfamily C, member 17 mm
MOE430B:1446928_AT DnaJ (Hsp40) homolog, subfamily C, member 17 mm
MOUSE430_2:1446928_AT DnaJ (Hsp40) homolog, subfamily C, member 17 mm
MOUSE430_2:1456784_AT DnaJ (Hsp40) homolog, subfamily C, member 17 (Dnajc17), mRNA mm
MOE430B:1456784_AT DnaJ (Hsp40) homolog, subfamily C, member 17 (Dnajc17), mRNA mm
MG-U74BV2:105080_AT DnaJ (Hsp40) homolog, subfamily C, member 17 (Dnajc17), mRNA mm
MOUSE430_2:1449756_AT DnaJ (Hsp40) homolog, subfamily C, member 17 (Dnajc17), mRNA mm
MOE430A:1449756_AT DnaJ (Hsp40) homolog, subfamily C, member 17 (Dnajc17), mRNA mm
MOUSE430A_2:1449756_AT DnaJ (Hsp40) homolog, subfamily C, member 17 (Dnajc17), mRNA mm
RG-U34A:RC_AA874839_AT similar to RIKEN cDNA 1700025B16 (predicted) rn
RAE230A:1374291_AT similar to RIKEN cDNA 1700025B16 (predicted) rn
RAT230_2:1374291_AT similar to RIKEN cDNA 1700025B16 (predicted) rn
RAE230A:1391166_AT Similar to RIKEN cDNA 1700025B16 (predicted) rn
RAT230_2:1391166_AT Similar to RIKEN cDNA 1700025B16 (predicted) rn
RG-U34A:RC_AA800146_AT Similar to RIKEN cDNA 1700025B16 (predicted) rn
RG-U34B:RC_AI029839_AT Similar to RIKEN cDNA 1700025B16 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAB69938 Dnaj domain (prokaryotic heat shock protein) protein 22 [Caenorhabditis elegans] ref|NP_505178.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-22) [Caenorhabditis elegans] 1.0E-164
blast CAE58390 Hypothetical protein CBG01519 [Caenorhabditis briggsae] 1.0E-123
blast EAL29093 GA14374-PA [Drosophila pseudoobscura] 2.0E-21
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 6.99999990710563E-19
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.0E-26
Sequence
Target Sequence 
>C. ELEGANS:173712_S_AT
aacggtcagcagatggatcaactccaatgacagcagcacaacaagctaaaaagaagaaaa
ccgatcaaagaaactttaaagaagaaattgaagcaattcggaggcagttggagaaagaag
tcaacgaagaggtgaaacagaaggcaacattgatgaaaacagaaagggaaaagcatcaga
aaagtcaagaaaaattaactcctcgattacttttgaaatggaaaacatcagaaggattag
actattctgaagacgatattcgaaaattattttcgacttttggaatcatatctcacatat
cttcggcaatagagaaaaaagatcgacggaaacgaatcctcgaatttgagtcgggagcaa
atgcatggggagctgaacttgaaactgggaatgctccgatgcctgaaattacgtgtgaat
ggttacaaccaccggtggaaactgtgaagaaagcttcggatgagccagtaaaatcaacta
atacgggtgccaacaatttggagcacatgtctttggacgatctcgaagcacaaattatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGGTCAGCAGATGGATCAACTCC 600 143 354 Antisense
GATCAACTCCAATGACAGCAGCACA 680 421 369 Antisense
TTAACTCCTCGATTACTTTTGAAAT 576 683 548 Antisense
GAATCATATCTCACATATCTTCGGC 162 329 636 Antisense
GAATCCTCGAATTTGAGTCGGGAGC 595 327 687 Antisense
GCATGGGGAGCTGAACTTGAAACTG 480 309 716 Antisense
GAAACTGGGAATGCTCCGATGCCTG 668 357 734 Antisense
GCTCCGATGCCTGAAATTACGTGTG 675 297 746 Antisense
AAATCAACTAATACGGGTGCCAACA 707 115 824 Antisense
GGAGCACATGTCTTTGGACGATCTC 73 521 853 Antisense
GGACGATCTCGAAGCACAAATTATG 184 523 868 Antisense
Sequence Source Affymetrix Proprietary Database