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[173703_s_at]

Description AU115242_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F25H5.6 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AU115242 /WB_GENE_ID=F25H5.6 /WP=CE15902 /CHR=1 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk729f9 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 54      4grp_highest_8_8 > 11      4grp_high_8_8 > 39      6grp_highest_nonorm > 52      6grp_total > 44      4grp_total > 40      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 500
1 2 3 4 range
A 545.5
(-3)
P 		836.7
(2.9)
P 		760.9
(1.4)
P 		840.6
(3)
P 		295
B 599.2
(-3)
P 		832.5
(0.1)
P 		1045.9
(3)
P 		769.8
(-0.7)
P 		447
C 664.4
(-3)
P 		855.6
(1.7)
P 		906.8
(3)
P 		761.6
(-0.6)
P 		242
avr 603
(-3) 		841.6
(1.7) 		904.5
(3) 		790.7
(0.7) 		302

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 529
1 2 3 4 5 6 range
A 576.9
(-1)
P 		654.7
(0.3)
P 		829.7
(3)
P 		546.6
(-1.4)
P 		461.3
(-2.8)
P 		447.7
(-3)
P 		382
B 625.3
(-1.5)
P 		766.8
(0.4)
P 		961.8
(3)
P 		558.6
(-2.4)
P 		509.8
(-3)
P 		616
(-1.6)
P 		452
C 616.8
(-0.4)
P 		861.8
(3)
P 		571.7
(-1.1)
P 		566.2
(-1.1)
P 		433.3
(-3)
P 		645
(-0)
P 		429
avr 606.3
(-0.4) 		761.1
(2.5) 		787.7
(3) 		557.1
(-1.3) 		468.1
(-3) 		569.6
(-1.1) 		320
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 193
1 2 3 4 range
A 198.6
(3)
P 		181.6
(1.6)
P 		198.9
(3)
P 		123.1
(-3)
P 		76
B 191.1
(-0.9)
P 		205.6
(0.2)
P 		242.3
(3)
P 		164
(-3)
P 		78
C 219.9
(-0.1)
P 		246
(0.8)
P 		316.1
(3)
P 		128.4
(-3)
P 		188
avr 203.2
(0.4) 		211.1
(0.8) 		252.4
(3) 		138.5
(-3) 		114

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 157
1 2 3 4 5 6 range
A 106.3
(-3)
P 		232.9
(3)
P 		180.7
(0.5)
P 		138.6
(-1.5)
P 		139.2
(-1.4)
P 		109
(-2.9)
P 		127
B 217.6
(0.9)
P 		255.2
(3)
P 		233.6
(1.8)
P 		148
(-3)
P 		207.1
(0.3)
P 		229.7
(1.6)
P 		107
C 228.6
(1.6)
P 		263
(3)
P 		145.5
(-1.7)
P 		113.9
(-3)
P 		140.2
(-1.9)
P 		215.9
(1.1)
P 		149
avr 184.2
(-0.4) 		250.4
(3) 		186.6
(-0.3) 		133.5
(-3) 		162.2
(-1.5) 		184.9
(-0.4) 		117
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173703_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.22584
Sequence Type Exemplar sequence
Representative Public ID AU115242 NCBI
Target Description AU115242_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F25H5.6 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AU115242 /WB_GENE_ID=F25H5.6 /WP=CE15902 /CHR=1 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk729f9 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060054(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060054 NCBI Caenorhabditis elegans F25H5.6 (F25H5.6) mRNA, complete cds. 11/11 None
SNAP00000024317 ENSEMBL cdna:SNAP chromosome:CEL140:I:9167245:9167646:-1 11/11 None
GENEFINDER00000024334 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9167245:9167909:-1 11/11 None
F25H5.6.1 ENSEMBL cdna:known chromosome:CEL140:I:9167139:9167646:-1 gene:F25H5.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9167156-9167841(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25H5.6
Entrez Gene 172741 Entrez gene
WormBase CE29591 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02987 Hypothetical protein F25H5.6 [Caenorhabditis elegans] ref|NP_492455.1| F25H5.6 [Caenorhabditis elegans] 3.0E-58
blast CAE70382 Hypothetical protein CBG16943 [Caenorhabditis briggsae] 2.0E-50
blast XP_216854 PREDICTED: similar to mitochondrial ribosomal protein L54 [Rattus norvegicus] 5.0E-10
Sequence
Target Sequence 
>C. ELEGANS:173703_S_AT
ctccgttcgactctttcaacagtcagtcgaactacatcactgatacctcgacgcttatat
gctccaccaaaagctgcagcagctgctgcaggtgtaaaagtcgacaaatctttcgtcgaa
gaagatgctgtcaaattggcaacccacgtatgcatcaatgcatacacacaaggagatgag
ccgggaccgaaaattcttccagactcggagtatcctgaatggctgttcaaattggatctt
cgagcaccaagagatttagaagatttggatgccgaaaaggatggatggttgtactggaga
gctctaagaaaaagacaagtcgagcaaaatcaacgaattcagaagttgaagactaagttc
ctacacttgcagaatagtccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCCGTTCGACTCTTTCAACAGTCA 645 231 83 Antisense
CAACAGTCAGTCGAACTACATCACT 472 189 99 Antisense
CACTGATACCTCGACGCTTATATGC 125 199 120 Antisense
GCTCCACCAAAAGCTGCAGCAGCTG 567 297 143 Antisense
GATGCTGTCAAATTGGCAACCCACG 51 415 206 Antisense
GGCAACCCACGTATGCATCAATGCA 228 535 220 Antisense
GAGCCGGGACCGAAAATTCTTCCAG 592 385 260 Antisense
AAATTCTTCCAGACTCGGAGTATCC 53 117 273 Antisense
CTCGGAGTATCCTGAATGGCTGTTC 254 231 286 Antisense
GGATGGTTGTACTGGAGAGCTCTAA 421 515 365 Antisense
GTTCCTACACTTGCAGAATAGTCCC 360 441 439 Antisense
Sequence Source Affymetrix Proprietary Database