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[173696_at]

Description CEK014H8R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F21H12.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D27599 /WB_GENE_ID=F21H12.5 /WP=CE01916 /CHR=2 /FEA=Genomic Cluster /GEN=fbf-2 /DEF=C.elegans cDNA clone yk14h8 : 3prime end, single read.
Cluster 6grp_high1_10_10 > 99      4grp_highest_8_8 > 19      4grp_high_8_8 > 63      6grp_total > 94      4grp_total > 48      6grp_cgc > 89      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 129
1 2 3 4 range
A 70.7
(-3)
P
85.4
(-2.3)
P
199.4
(3)
P
118.7
(-0.8)
P
129
B 89.8
(-2.4)
P
77.5
(-3)
P
191.4
(3)
P
111.2
(-1.2)
P
114
C 113
(-1.3)
P
169.2
(1.7)
P
194.9
(3)
P
79.8
(-3)
P
115
avr 91.2
(-3)
110.7
(-1.9)
195.2
(3)
103.2
(-2.3)
104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 695
1 2 3 4 5 6 range
A 76.7
(-2.9)
A
68.8
(-3)
P
71.2
(-3)
P
269
(-1.2)
P
733.8
(3)
P
345.4
(-0.5)
P
665
B 39.3
(-3)
P
59.4
(-2.8)
P
91.4
(-2.4)
A
259
(-0.5)
P
498.6
(2.2)
P
571.2
(3)
P
532
C 38.6
(-3)
P
44.5
(-2.9)
P
68.7
(-2.7)
P
64.1
(-2.7)
A
602.9
(3)
P
203.5
(-1.2)
P
564
avr 51.5
(-3)
57.6
(-2.9)
77.1
(-2.7)
197.4
(-1.4)
611.8
(3)
373.4
(0.4)
560
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 25.8
(-1.5)
P
18.5
(-2.8)
P
52.1
(3)
P
17.4
(-3)
P
35
B 28.7
(-0.7)
P
19.1
(-3)
P
44.3
(3)
P
23.7
(-1.9)
P
25
C 37.4
(-0.4)
P
48.7
(0.9)
P
67.9
(3)
P
13.5
(-3)
P
54
avr 30.6
(-1)
28.8
(-1.3)
54.8
(3)
18.2
(-3)
37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 209
1 2 3 4 5 6 range
A 14.1
(-3)
A
24.5
(-2.7)
P
15.5
(-3)
P
68.2
(-1.4)
P
221.4
(3)
P
84.1
(-1)
P
207
B 13.7
(-3)
P
19.8
(-2.8)
P
22.2
(-2.7)
A
68.6
(-1.3)
P
202.6
(2.7)
P
213
(3)
P
199
C 14.3
(-3)
P
13.6
(-3)
P
17.5
(-2.8)
P
12.9
(-3)
A
195.1
(3)
P
68.1
(-1.2)
P
182
avr 14
(-3)
19.3
(-2.8)
18.4
(-2.9)
49.9
(-1.9)
206.4
(3)
121.7
(0.4)
192
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173696_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23729
Sequence Type Exemplar sequence
Representative Public ID CEK014H8R NCBI
Target Description CEK014H8R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F21H12.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D27599 /WB_GENE_ID=F21H12.5 /WP=CE01916 /CHR=2 /FEA=Genomic Cluster /GEN=fbf-2 /DEF=C.elegans cDNA clone yk14h8 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062819(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_062819 NCBI Caenorhabditis elegans Fem-3 mRNA Binding Factor family member (fbf-2) (fbf-2) mRNA, complete cds. 11/11 None
F21H12.5 ENSEMBL cdna:known chromosome:CEL140:II:6088831:6091434:-1 gene:F21H12.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6088838-6091324(-) 94.89 100.0
Public Domain and Genome References
Gene Title
Gene Symbol fbf-2
Ensembl F21H12.5
Entrez Gene 174017 Entrez gene
SwissProt Q09312 EMBL-EBI
WormBase CE01916 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_495220 Fem-3 mRNA Binding Factor family member (fbf-2) [Caenorhabditis elegans] gb|AAC46716.1| Fem-3 mrna binding factor protein 2 [Caenorhabditis elegans] sp|Q09312|FBF2_CAEEL Fem-3 mRNA binding factor 0.0
blast AAF39879 Fem-3 mrna binding factor protein 1 [Caenorhabditis elegans] ref|NP_495216.1| Fem-3 mRNA Binding Factor family member (fbf-1) [Caenorhabditis elegans] sp|Q9N5M6|FBF1_CAEEL Fem-3 mRNA binding factor 0.0
blast CAE59353 Hypothetical protein CBG02702 [Caenorhabditis briggsae] 1.0E-112
Protein Domains
Database ID Description E-Value
Pfam IPR001313 EMBL-EBI Pumilio/Puf RNA-binding 3.3E-6
Sequence
Target Sequence
>C. ELEGANS:173696_AT
gattaaggtggaactttctcaccataaatctcatcccacccacttatgttctgttgtttt
gtaatattttaaactggtgcaatgatttcgtttcgattttaagatcgatgttgtgccttc
cccactcttatcattgcccagttttgttttttcttttcttttttgttattttattgcttt
ttatgcagttgtgttattgtttctttctgtttactgttcattgtttttgt


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
GATTAAGGTGGAACTTTCTCACCAT 47 429 1907 Antisense
CCACCCACTTATGTTCTGTTGTTTT 88 273 1942 Antisense
CCCACTTATGTTCTGTTGTTTTGTA 474 257 1945 Antisense
TTTTAAACTGGTGCAATGATTTCGT 109 667 1973 Antisense
ATGATTTCGTTTCGATTTTAAGATC 350 45 1988 Antisense
TTAAGATCGATGTTGTGCCTTCCCC 525 683 2005 Antisense
CACTCTTATCATTGCCCAGTTTTGT 412 197 2029 Antisense
TATTGCTTTTTATGCAGTTGTGTTA 184 665 2078 Antisense
GTTGTGTTATTGTTTCTTTCTGTTT 510 437 2094 Antisense
GTTTCTTTCTGTTTACTGTTCATTG 57 451 2105 Antisense
TCTGTTTACTGTTCATTGTTTTTGT 280 609 2112 Antisense
Sequence Source Affymetrix Proprietary Database