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[173678_s_at]

Description AU110306 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F25G6.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AU110306 /WB_GENE_ID=F25G6.8 /WP=CE09644 /CHR=5 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk718b1 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 02      6grp_high1_10_10 > 01      4grp_highest_8_8 > 10      4grp_high_8_8 > 55      6grp_highest_nonorm > 72      6grp_total > 09      4grp_total > 57      6grp_high1_sub > De      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 261
1 2 3 4 range
A 292.3
(-3)
P 		470.6
(3)
P 		435.3
(1.8)
P 		327.5
(-1.8)
P 		178
B 299.1
(-3)
P 		337
(-1.9)
P 		502.5
(3)
P 		340.2
(-1.8)
P 		203
C 361.9
(-0.5)
P 		482
(2.3)
P 		512.7
(3)
P 		251.9
(-3)
P 		261
avr 317.8
(-2.6) 		429.9
(1.2) 		483.5
(3) 		306.5
(-3) 		177

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 319
1 2 3 4 5 6 range
A 456.1
(0.5)
P 		420.1
(-0.5)
P 		542.5
(3)
P 		426.1
(-0.3)
P 		332.9
(-3)
P 		395.4
(-1.2)
P 		210
B 422.9
(0.7)
P 		420.2
(0.6)
P 		464.1
(3)
P 		372.7
(-2)
P 		388.5
(-1.2)
P 		355
(-3)
P 		109
C 650.3
(3)
P 		331.4
(-3)
P 		399.4
(-1.7)
P 		544.6
(1)
P 		468.9
(-0.4)
P 		395.6
(-1.8)
P 		319
avr 509.8
(3) 		390.6
(-2.6) 		468.7
(1.1) 		447.8
(0.1) 		396.8
(-2.3) 		382
(-3) 		128
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 106.4
(2.3)
P 		102.1
(1.9)
P 		113.8
(3)
P 		47.9
(-3)
P 		66
B 95.4
(0.1)
P 		83.2
(-1.5)
P 		116.4
(3)
P 		72.5
(-3)
P 		44
C 119.8
(0.4)
P 		138.6
(1.2)
P 		178.7
(3)
P 		42.5
(-3)
P 		136
avr 107.2
(0.9) 		108
(0.9) 		136.3
(3) 		54.3
(-3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 157
1 2 3 4 5 6 range
A 84.1
(-3)
P 		149.4
(3)
P 		118.1
(0.1)
P 		108.1
(-0.8)
P 		100.5
(-1.5)
P 		96.3
(-1.9)
P 		65
B 147.1
(1.9)
P 		139.9
(1.2)
P 		112.7
(-1.6)
P 		98.7
(-3)
P 		157.9
(3)
P 		132.4
(0.4)
P 		59
C 241
(3)
P 		101.1
(-3)
P 		101.7
(-3)
P 		109.5
(-2.6)
P 		151.7
(-0.8)
P 		132.4
(-1.7)
P 		140
avr 157.4
(3) 		130.1
(-0.2) 		110.8
(-2.4) 		105.4
(-3) 		136.7
(0.6) 		120.4
(-1.3) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173678_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25132
Sequence Type Exemplar sequence
Representative Public ID AU110306 NCBI
Target Description AU110306 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F25G6.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AU110306 /WB_GENE_ID=F25G6.8 /WP=CE09644 /CHR=5 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk718b1 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072807(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072807 NCBI Caenorhabditis elegans F25G6.8 (F25G6.8) mRNA, complete cds. 11/11 None
SNAP00000003981 ENSEMBL cdna:SNAP chromosome:CEL140:V:8567156:8567759:-1 11/11 None
GENEFINDER00000003986 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8567156:8567759:-1 11/11 None
F25G6.8.2 ENSEMBL cdna:known chromosome:CEL140:V:8566700:8567765:-1 gene:F25G6.8 11/11 A 
F25G6.8.1 ENSEMBL cdna:known chromosome:CEL140:V:8566891:8567763:-1 gene:F25G6.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8566971-8567761(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25G6.8
Entrez Gene 179236 Entrez gene
SwissProt O16927 EMBL-EBI
WormBase CE09644 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6605 protein targeting inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5786 signal recognition particle (sensu Eukaryota) inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC25801 Hypothetical protein F25G6.8 [Caenorhabditis elegans] ref|NP_505208.1| F25G6.8 [Caenorhabditis elegans] sp|O16927|SRP14_CAEEL Signal recognition particle 14 kDa protein homolog (SRP14) 1.0E-50
blast CAE58365 Hypothetical protein CBG01490 [Caenorhabditis briggsae] 6.0E-47
Protein Domains
Database ID Description E-Value
Pfam IPR003210 EMBL-EBI Signal recognition particle, 14 kDa protein 2.9E-65
Sequence
Target Sequence 
>C. ELEGANS:173678_S_AT
acaacatattcccaacgaccaattcctgcaaaagctgacggcattttatagagattcaaa
gattcgaggaccaaaaagtgtatatgttacaatgaagccagatgacggtcgaaccaaagc
gatgccaaaaggaagcacgttcaaagagggtgacgaaatttcatgtatctttcgagccaa
atggggaagcaaaaagattgccacagaggtgaaagcaaaagaagttaacaagttccacac
tcaatattctgccataattgtcgcacaaatggtcaacttggagaagaggaaaaagacgga
cgatgagaaaaagaagacaggtgccacgaaggcctaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAACATATTCCCAACGACCAATTC 103 113 28 Antisense
ACCAATTCCTGCAAAAGCTGACGGC 445 81 45 Antisense
AGCTGACGGCATTTTATAGAGATTC 620 79 60 Antisense
GACGGTCGAACCAAAGCGATGCCAA 431 377 131 Antisense
GCGATGCCAAAAGGAAGCACGTTCA 77 291 146 Antisense
GGGTGACGAAATTTCATGTATCTTT 21 499 175 Antisense
ATGTATCTTTCGAGCCAAATGGGGA 472 47 190 Antisense
GAAGTTAACAAGTTCCACACTCAAT 446 337 248 Antisense
AAGTTCCACACTCAATATTCTGCCA 694 155 257 Antisense
TATTCTGCCATAATTGTCGCACAAA 217 665 272 Antisense
GAAGACAGGTGCCACGAAGGCCTAA 568 333 340 Antisense
Sequence Source Affymetrix Proprietary Database