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[173675_at]

Description AV195724 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F59A3.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV195724 /WB_GENE_ID=F59A3.4 /WP=CE11422 /CHR=1 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk645b8 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 89      4grp_highest_8_8 > 03      4grp_high_8_8 > 46      6grp_highest_nonorm > 85      6grp_total > 95      4grp_total > 41      6grp_high1_sub > Cu      6grp_total_sub > Increase      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 455
1 2 3 4 range
A 611.3
(-3)
P 		987.6
(2.3)
P 		1039
(3)
P 		714.6
(-1.6)
P 		428
B 680.2
(-3)
P 		875.3
(3)
P 		749.1
(-0.9)
P 		783.2
(0.2)
P 		195
C 584.4
(-3)
P 		773.8
(2.2)
P 		803.2
(3)
P 		730.2
(1)
P 		219
avr 625.3
(-3) 		878.9
(3) 		863.8
(2.6) 		742.7
(-0.2) 		254

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 644
1 2 3 4 5 6 range
A 875.5
(1)
P 		651
(-1.9)
P 		564.7
(-3)
P 		731
(-0.8)
P 		1028
(3)
P 		814.6
(0.2)
P 		463
B 598
(-2.1)
P 		679.2
(-1.3)
P 		499.8
(-3)
P 		867.8
(0.4)
P 		1019.1
(1.8)
P 		1144.1
(3)
P 		644
C 896.5
(0.4)
P 		703.7
(-2.9)
P 		695.7
(-3)
P 		753.1
(-2)
P 		1051.8
(3)
P 		908.2
(0.6)
P 		356
avr 790
(-0.3) 		678
(-1.8) 		586.7
(-3) 		784
(-0.3) 		1033
(3) 		955.6
(2) 		446
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 175
1 2 3 4 range
A 222.6
(1.2)
P 		214.4
(0.9)
P 		271.6
(3)
P 		104.6
(-3)
P 		167
B 216.9
(3)
P 		216.2
(2.9)
P 		173.6
(-2.2)
P 		166.9
(-3)
P 		50
C 193.5
(-0.3)
P 		222.5
(0.8)
P 		280
(3)
P 		123.1
(-3)
P 		157
avr 211
(1.3) 		217.7
(1.7) 		241.7
(3) 		131.5
(-3) 		110

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 305
1 2 3 4 5 6 range
A 161.4
(-1.8)
P 		231.5
(0.5)
P 		123
(-3)
P 		185.4
(-1)
P 		310.2
(3)
P 		198.3
(-0.6)
P 		187
B 208.1
(-1.3)
P 		226.1
(-0.9)
P 		121.4
(-3)
P 		229.9
(-0.9)
P 		414
(2.8)
P 		426.6
(3)
P 		305
C 332.3
(2.7)
P 		214.7
(-1)
P 		177.1
(-2.2)
P 		151.5
(-3)
P 		340.3
(3)
P 		304
(1.8)
P 		189
avr 233.9
(-0.4) 		224.1
(-0.7) 		140.5
(-3) 		188.9
(-1.6) 		354.8
(3) 		309.6
(1.7) 		214
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173675_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23016
Sequence Type Exemplar sequence
Representative Public ID AV195724 NCBI
Target Description AV195724 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F59A3.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV195724 /WB_GENE_ID=F59A3.4 /WP=CE11422 /CHR=1 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk645b8 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059213(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059213 NCBI Caenorhabditis elegans F59A3.4 (F59A3.4) mRNA, complete cds. 11/11 None
F59A3.4 ENSEMBL cdna:known chromosome:CEL140:I:5507126:5509172:-1 gene:F59A3.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5507151-5509173(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F59A3.4
Entrez Gene 172205 Entrez gene
SwissProt P91354 EMBL-EBI
WormBase CE11422 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.7522.1.S1_S_AT similar to solute carrier family 39 (metal ion transporter), member 11 cfa
DROSOPHILA_2:1638312_AT dm
DROSGENOME1:154474_AT dm
CHICKEN:GGAAFFX.26205.1.S1_S_AT similar to solute carrier family 39 (metal ion transporter), member 11 gga
HU35KSUBA:AA044095_AT solute carrier family 39 (metal ion transporter), member 11 hs
HU35KSUBA:RC_AA039887_AT solute carrier family 39 (metal ion transporter), member 11 hs
U133_X3P:HS.3402.0.A1_3P_AT solute carrier family 39 (metal ion transporter), member 11 hs
HG-U133_PLUS_2:227046_AT solute carrier family 39 (metal ion transporter), member 11 hs
HG-U133B:227046_AT solute carrier family 39 (metal ion transporter), member 11 hs
HG-U95B:51230_AT solute carrier family 39 (metal ion transporter), member 11 hs
HG-U95D:75885_AT solute carrier family 39 (metal ion transporter), member 11 hs
HG-U133_PLUS_2:234567_AT Solute carrier family 39 (metal ion transporter), member 11 hs
HG-U133B:234567_AT Solute carrier family 39 (metal ion transporter), member 11 hs
HG-U95C:54687_AT Solute carrier family 39 (metal ion transporter), member 11 hs
HG-U95E:72067_AT Solute carrier family 39 (metal ion transporter), member 11 hs
HG-U95D:72103_AT Solute carrier family 39 (metal ion transporter), member 11 hs
HG-U95D:72938_AT Solute carrier family 39 (metal ion transporter), member 11 hs
HG-U95D:85942_AT Solute carrier family 39 (metal ion transporter), member 11 hs
HU35KSUBC:RC_D20379_AT Solute carrier family 39 (metal ion transporter), member 11 hs
MOUSE430A_2:1424905_A_AT solute carrier family 39 (metal ion transporter), member 11 mm
MOE430A:1424905_A_AT solute carrier family 39 (metal ion transporter), member 11 mm
MOUSE430_2:1424905_A_AT solute carrier family 39 (metal ion transporter), member 11 mm
MG-U74BV2:114625_AT solute carrier family 39 (metal ion transporter), member 11 mm
MU19KSUBC:TC36076_AT Solute carrier family 39 (metal ion transporter), member 11, mRNA (cDNA clone MGC:25889 IMAGE:4216487) mm
MU19KSUBC:TC41071_AT Solute carrier family 39 (metal ion transporter), member 11, mRNA (cDNA clone MGC:25889 IMAGE:4216487) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
30001 metal ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
46873 metal ion transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB37816 Hypothetical protein F59A3.4 [Caenorhabditis elegans] ref|NP_491614.1| F59A3.4 [Caenorhabditis elegans] 1.0E-179
blast CAE67074 Hypothetical protein CBG12483 [Caenorhabditis briggsae] 1.0E-170
blast XP_852551 PREDICTED: similar to solute carrier family 39 (metal ion transporter), member 11 [Canis familiaris] 6.0E-93
Protein Domains
Database ID Description E-Value
Pfam IPR003689 EMBL-EBI Zinc transporter ZIP 3.4E-29
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491614.1 8 10-32,39-61,74-93,176-198,213-235,242-264,269-286,298-320
F59A3.4 8 10-32,39-61,74-93,176-198,213-235,242-264,269-286,298-320
Sequence
Target Sequence 
>C. ELEGANS:173675_AT
ctcggaaacttgttcacaaccccattacaacaatttgcgagttcataatgttttctgtga
aaaacttcatttttattccaaaattttaccattttctgtgataatttctcttcgtattac
gcatttttttaccggattcgaaatcttcaatgtgccacgcattcctttcccctcttcata
ataactcttattaacggtattcttttgcccctgcataattcatcattt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCGGAAACTTGTTCACAACCCCAT 265 227 981 Antisense
ACAACCCCATTACAACAATTTGCGA 16 113 996 Antisense
TGCGAGTTCATAATGTTTTCTGTGA 348 583 1016 Antisense
TCCAAAATTTTACCATTTTCTGTGA 213 587 1057 Antisense
TTTTCTGTGATAATTTCTCTTCGTA 393 677 1072 Antisense
ATTTCTCTTCGTATTACGCATTTTT 38 17 1084 Antisense
GGATTCGAAATCTTCAATGTGCCAC 176 509 1114 Antisense
AAATCTTCAATGTGCCACGCATTCC 8 119 1121 Antisense
TTTCCCCTCTTCATAATAACTCTTA 653 667 1146 Antisense
TATTAACGGTATTCTTTTGCCCCTG 424 663 1169 Antisense
TTTGCCCCTGCATAATTCATCATTT 173 669 1184 Antisense
Sequence Source Affymetrix Proprietary Database