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[173674_at]

Description AV192094 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T04F8.2 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV192094 /WB_GENE_ID=T04F8.2 /WP=CE23945 /CHR=X /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk601d3 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 17      4grp_highest_8_8 > 36      4grp_high_8_8 > 08      6grp_highest_nonorm > 81      6grp_total > 07      4grp_total > 15      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 158
1 2 3 4 range
A 275.6
(3)
P 		117.5
(-3)
P 		177.7
(-0.7)
P 		196.5
(-0)
P 		158
B 183.1
(-3)
P 		212.3
(-0)
P 		185.1
(-2.8)
P 		242
(3)
P 		59
C 259.3
(2)
P 		237.6
(-0.5)
P 		267.9
(3)
P 		215.7
(-3)
P 		52
avr 239.3
(3) 		189.1
(-3) 		210.2
(-0.5) 		218.1
(0.5) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 294
1 2 3 4 5 6 range
A 210.2
(0.5)
P 		199.9
(0.1)
P 		277.1
(3)
P 		190.8
(-0.3)
P 		230.4
(1.2)
P 		119
(-3)
P 		158
B 413.2
(3)
P 		295.2
(-1.1)
P 		283.1
(-1.5)
P 		240.1
(-3)
P 		283.8
(-1.5)
P 		306.9
(-0.7)
P 		173
C 231.1
(0.2)
P 		243.1
(0.9)
P 		175.7
(-3)
P 		279.6
(3)
P 		200
(-1.6)
P 		276.1
(2.8)
P 		104
avr 284.8
(3) 		246.1
(-1.6) 		245.3
(-1.7) 		236.8
(-2.7) 		238.1
(-2.5) 		234
(-3) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 100.3
(3)
P 		25.5
(-3)
P 		46.4
(-1.3)
P 		28.8
(-2.7)
P 		75
B 58.4
(3)
P 		52.4
(0.7)
P 		42.9
(-3)
P 		51.6
(0.4)
P 		16
C 85.8
(2.2)
P 		68.3
(0.4)
P 		93.4
(3)
P 		36.4
(-3)
P 		57
avr 81.5
(3) 		48.7
(-1.6) 		60.9
(0.1) 		38.9
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 115
1 2 3 4 5 6 range
A 38.8
(-1.6)
P 		71.1
(3)
P 		60.3
(1.5)
P 		48.4
(-0.2)
P 		69.5
(2.8)
P 		29
(-3)
P 		42
B 143.8
(3)
P 		98.3
(-0.4)
P 		68.7
(-2.6)
P 		63.6
(-3)
P 		115.3
(0.9)
P 		114.5
(0.8)
P 		80
C 85.6
(2.1)
P 		74.2
(0.7)
P 		44.7
(-3)
P 		56.2
(-1.6)
P 		64.7
(-0.5)
P 		92.4
(3)
P 		48
avr 89.4
(3) 		81.2
(1.5) 		57.9
(-2.7) 		56.1
(-3) 		83.2
(1.9) 		78.6
(1.1) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173674_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25812
Sequence Type Exemplar sequence
Representative Public ID AV192094 NCBI
Target Description AV192094 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T04F8.2 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AV192094 /WB_GENE_ID=T04F8.2 /WP=CE23945 /CHR=X /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk601d3 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077546(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077546 NCBI Caenorhabditis elegans T04F8.2 (T04F8.2) mRNA, complete cds. 11/11 None
T04F8.2.3 ENSEMBL cdna:known chromosome:CEL140:X:11653149:11656491:-1 gene:T04F8.2 11/11 A 
T04F8.2.2 ENSEMBL cdna:known chromosome:CEL140:X:11653149:11656491:-1 gene:T04F8.2 11/11 A 
T04F8.2.1 ENSEMBL cdna:known chromosome:CEL140:X:11653491:11656491:-1 gene:T04F8.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11653597-11656480(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T04F8.2
Entrez Gene 181352 Entrez gene
SwissProt Q22162 EMBL-EBI
WormBase CE23945 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1627502_AT dm
DROSGENOME1:154379_AT dm
Protein Similarities
Method ID Description E-Value
blast CAA91478 Hypothetical protein T04F8.2 [Caenorhabditis elegans] ref|NP_509947.1| T04F8.2 [Caenorhabditis elegans] 0.0
blast CAE70601 Hypothetical protein CBG17275 [Caenorhabditis briggsae] 0.0
blast XP_624091 PREDICTED: similar to ENSANGP00000017930 [Apis mellifera] 3.0E-70
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509947.1 6 217-239,259-281,321-343,352-374,394-416,423-445
T04F8.2.3 6 217-239,259-281,321-343,352-374,394-416,423-445
T04F8.2.2 6 217-239,259-281,321-343,352-374,394-416,423-445
T04F8.2.1 6 217-239,259-281,321-343,352-374,394-416,423-445
Sequence
Target Sequence 
>C. ELEGANS:173674_AT
cgaactcagatctagcaaattgtcataatatcatcattttcttcaaaactaggacttttc
ctttttccacttttcttgtatattttgcttccctgtcagccatgtatctgataacatatc
cagtttgcacctaatttcagtgactaatttgaacaaatgtttggtagttacgactatcaa
taattattcgctttgcaattttaaaggatagagtgattgttattatgtctattttctttt
ttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGAACTCAGATCTAGCAAATTGTCA 688 251 1508 Antisense
AGCAAATTGTCATAATATCATCATT 174 77 1521 Antisense
TTGTATATTTTGCTTCCCTGTCAGC 583 705 1583 Antisense
CTTCCCTGTCAGCCATGTATCTGAT 46 221 1595 Antisense
GCCATGTATCTGATAACATATCCAG 631 279 1606 Antisense
ACATATCCAGTTTGCACCTAATTTC 282 105 1621 Antisense
GTTTGCACCTAATTTCAGTGACTAA 584 451 1630 Antisense
ATGTTTGGTAGTTACGACTATCAAT 145 47 1664 Antisense
GACTATCAATAATTATTCGCTTTGC 314 373 1679 Antisense
GAGTGATTGTTATTATGTCTATTTT 502 401 1718 Antisense
TTATTATGTCTATTTTCTTTTTTCT 569 679 1727 Antisense
Sequence Source Affymetrix Proprietary Database