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[173671_at]

Description AU109258 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F33D4.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AU109258 /WB_GENE_ID=F33D4.4 /WP=CE17034 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk707h4 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 71      4grp_highest_8_8 > 42      4grp_high_8_8 > 48      6grp_highest_nonorm > 66      6grp_total > 71      4grp_total > 55      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 998
1 2 3 4 range
A 1894.9
(3)
P 		1032.6
(-2.9)
P 		1575.8
(0.8)
P 		1013
(-3)
P 		882
B 2002.6
(3)
P 		1334.5
(-1.4)
P 		1091.7
(-3)
P 		1447.9
(-0.7)
P 		911
C 1548.5
(3)
P 		1184.4
(-1)
P 		1383.7
(1.2)
P 		1004.2
(-3)
P 		544
avr 1815.3
(3) 		1183.8
(-2.7) 		1350.4
(-1.2) 		1155
(-3) 		660

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 842
1 2 3 4 5 6 range
A 1333.4
(-3)
P 		1355
(-2.6)
P 		1412
(-1.5)
P 		1448.2
(-0.8)
P 		1640.3
(3)
P 		1340.6
(-2.9)
P 		307
B 1293.8
(-3)
P 		1503.1
(-1.4)
P 		1358.2
(-2.5)
P 		2092.5
(3)
P 		1439.9
(-1.9)
P 		1333.5
(-2.7)
P 		799
C 1250.5
(-3)
P 		1529.9
(0.2)
P 		1780.7
(3)
P 		1667.7
(1.7)
P 		1300.1
(-2.4)
P 		1363.3
(-1.7)
P 		530
avr 1292.6
(-3) 		1462.7
(-0.7) 		1517
(0) 		1736.1
(3) 		1460.1
(-0.7) 		1345.8
(-2.3) 		444
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 542
1 2 3 4 range
A 689.9
(3)
P 		224.2
(-2.2)
P 		411.9
(-0.1)
P 		148.3
(-3)
P 		542
B 638.6
(3)
P 		329.6
(-1.8)
P 		253
(-3)
P 		308.5
(-2.1)
P 		386
C 512.6
(3)
P 		340.5
(-0)
P 		482.3
(2.5)
P 		169.3
(-3)
P 		343
avr 613.7
(3) 		298.1
(-1.7) 		382.4
(-0.4) 		208.7
(-3) 		405

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 339
1 2 3 4 5 6 range
A 245.8
(-3)
P 		481.9
(2.7)
P 		307.5
(-1.5)
P 		367.3
(-0.1)
P 		495
(3)
P 		326.4
(-1.1)
P 		249
B 450.2
(-0.2)
P 		500.3
(1)
P 		329.8
(-3)
P 		554.2
(2.3)
P 		585
(3)
P 		497.3
(0.9)
P 		255
C 463.5
(2.8)
P 		466.8
(3)
P 		453.2
(2.4)
P 		335.4
(-3)
P 		420.7
(0.9)
P 		456.4
(2.5)
P 		131
avr 386.5
(-2) 		483
(2.2) 		363.5
(-3) 		419
(-0.6) 		500.2
(3) 		426.7
(-0.2) 		137
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173671_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24532
Sequence Type Exemplar sequence
Representative Public ID AU109258 NCBI
Target Description AU109258 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F33D4.4 /5_PRIME_EXT_DB=WormBase Gene ID /GB=AU109258 /WB_GENE_ID=F33D4.4 /WP=CE17034 /CHR=4 /FEA=Genomic Cluster /DEF=Caenorhabditis elegans cDNA clone:yk707h4 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068855(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068855 NCBI Caenorhabditis elegans F33D4.4 (F33D4.4) mRNA, complete cds. 11/11 None
F33D4.4.2 ENSEMBL cdna:known chromosome:CEL140:IV:7703872:7706889:-1 gene:F33D4.4 11/11 None
F33D4.4.1 ENSEMBL cdna:known chromosome:CEL140:IV:7703872:7706888:-1 gene:F33D4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7703935-7706893(-) 98.59 100.0
Public Domain and Genome References
Gene Title
Ensembl F33D4.4
Entrez Gene 177548 Entrez gene
SwissProt O44186 EMBL-EBI
WormBase CE17034 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:255276_AT fatty acid desaturase family protein at
CANINE:1586012_AT similar to degenerative spermatocyte homolog 1, lipid desaturase cfa
CANINE_2:CFA.12492.1.S1_AT similar to degenerative spermatocyte homolog 1, lipid desaturase cfa
CANINE_2:CFAAFFX.25085.1.S1_S_AT similar to degenerative spermatocyte homolog 1, lipid desaturase cfa
DROSOPHILA_2:1625414_AT infertile crescent dm
DROSGENOME1:153842_AT infertile crescent dm
CHICKEN:GGA.8162.1.S1_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) gga
HG-U133_PLUS_2:207431_S_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HG-U133A:207431_S_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HG-U133A_2:207431_S_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HG-U133_PLUS_2:209250_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HG-U133A:209250_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HG-FOCUS:209250_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HG-U133A_2:209250_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HU35KSUBA:AF002668_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HU35KSUBD:RC_AA186666_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
U133_X3P:G4505192_3P_A_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
U133_X3P:G12803018_3P_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
HG-U95AV2:33337_AT degenerative spermatocyte homolog 1, lipid desaturase (Drosophila) hs
MU11KSUBB:Y08460_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MU11KSUBB:Y08460_G_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MG-U74AV2:95688_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MOE430A:1423345_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MOUSE430A_2:1423345_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MOUSE430_2:1423345_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MOE430A:1423346_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MOUSE430A_2:1423346_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MOUSE430_2:1423346_AT degenerative spermatocyte homolog 1 (Drosophila) mm
MU19KSUBA:TC20045_AT Degenerative spermatocyte homolog 1 (Drosophila), mRNA (cDNA clone MGC:5864 IMAGE:3600571) mm
MU19KSUBC:TC39637_S_AT Degenerative spermatocyte homolog 1 (Drosophila), mRNA (cDNA clone MGC:5864 IMAGE:3600571) mm
RG-U34C:RC_AI177412_AT degenerative spermatocyte homolog (Drosophila) rn
RAE230A:1367770_AT degenerative spermatocyte homolog (Drosophila) rn
RAT230_2:1367770_AT degenerative spermatocyte homolog (Drosophila) rn
RAE230B:1381384_AT Degenerative spermatocyte homolog (Drosophila) rn
RAT230_2:1381384_AT Degenerative spermatocyte homolog (Drosophila) rn
RAE230B:1386022_AT Degenerative spermatocyte homolog (Drosophila) rn
RAT230_2:1386022_AT Degenerative spermatocyte homolog (Drosophila) rn
RAE230B:1391406_AT Degenerative spermatocyte homolog (Drosophila) rn
RAT230_2:1391406_AT Degenerative spermatocyte homolog (Drosophila) rn
RG-U34C:RC_AI169572_AT Degenerative spermatocyte homolog (Drosophila) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB88372 Hypothetical protein F33D4.4 [Caenorhabditis elegans] ref|NP_501256.1| F33D4.4 [Caenorhabditis elegans] 0.0
blast CAE61942 Hypothetical protein CBG05940 [Caenorhabditis briggsae] 0.0
blast CAC70116 Hypothetical protein Y54E5A.1 [Caenorhabditis elegans] emb|CAA21659.2| Hypothetical protein Y54E5A.1 [Caenorhabditis elegans] ref|NP_493549.1| Y54E5A.1 [Caenorhabditis elegans] 1.0E-102
Protein Domains
Database ID Description E-Value
Pfam IPR005804 EMBL-EBI Fatty acid desaturase 2.3E-49
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501256.1 5 43-62,67-89,104-126,186-205,210-232
F33D4.4.2 5 43-62,67-89,104-126,186-205,210-232
F33D4.4.1 5 43-62,67-89,104-126,186-205,210-232
Sequence
Target Sequence 
>C. ELEGANS:173671_AT
atgcgactgatctttatgccaacacacaactttctaccaaaaactcgagccaaatgtaca
aactatggttttgattgttgttttattctcttttgatttcgttttcacatccatccacct
tctatattgttagtattatcattgccaaggtcatgcccttttttgattcccgattaattc
ccaaaattctacgcaaaattattgtttcctcgaaaccccgctggtaactctcaaagacca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCGACTGATCTTTATGCCAACAC 64 43 1087 Antisense
TTATGCCAACACACAACTTTCTACC 23 681 1100 Antisense
CACAACTTTCTACCAAAAACTCGAG 217 193 1111 Antisense
AACTCGAGCCAAATGTACAAACTAT 538 141 1128 Antisense
TTGATTTCGTTTTCACATCCATCCA 334 705 1179 Antisense
ATCCATCCACCTTCTATATTGTTAG 484 39 1195 Antisense
ATTATCATTGCCAAGGTCATGCCCT 496 13 1221 Antisense
CAAGGTCATGCCCTTTTTTGATTCC 653 181 1232 Antisense
TTGATTCCCGATTAATTCCCAAAAT 528 703 1249 Antisense
TACGCAAAATTATTGTTTCCTCGAA 501 647 1276 Antisense
CCCCGCTGGTAACTCTCAAAGACCA 433 259 1302 Antisense
Sequence Source Affymetrix Proprietary Database