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[173663_at]

Description C43062 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T28F3.1 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C43062 /WB_GENE_ID=T28F3.1 /WP=CE19806 /CHR=4 /FEA=Genomic Cluster /DEF=C2 domain; C.elegans cDNA clone yk335h8 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 98      6grp_high1_10_10 > 69      4grp_highest_8_8 > 62      4grp_high_8_8 > 27      6grp_highest_nonorm > 92      6grp_total > 49      4grp_total > 37      6grp_highest_sub > Cu      6grp_cgc > 09      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 124
1 2 3 4 range
A 215.3
(3)
P 		186.8
(1.5)
P 		97.6
(-3)
P 		198.5
(2.1)
P 		118
B 221.9
(3)
P 		208.2
(1.7)
P 		160.2
(-3)
P 		181.5
(-0.9)
P 		62
C 197
(-1)
P 		187
(-3)
P 		217.1
(3)
P 		197.1
(-1)
P 		30
avr 211.4
(3) 		194
(1) 		158.3
(-3) 		192.4
(0.9) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 397
1 2 3 4 5 6 range
A 249.1
(-0.9)
P 		187.4
(-2.5)
P 		165.6
(-3)
P 		181.7
(-2.6)
P 		407.3
(3)
P 		375.8
(2.2)
P 		242
B 328.7
(0.7)
P 		290.2
(0.1)
P 		85.6
(-3)
P 		147.5
(-2.1)
P 		354.9
(1.1)
P 		482.8
(3)
P 		397
C 328.4
(2.3)
P 		254.6
(0.6)
P 		92.4
(-3)
P 		359.9
(3)
P 		248.9
(0.5)
P 		294.5
(1.5)
P 		268
avr 302.1
(1.2) 		244.1
(-0.1) 		114.5
(-3) 		229.7
(-0.4) 		337
(1.9) 		384.4
(3) 		270
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 78.4
(3)
P 		40.5
(-1.3)
P 		25.5
(-3)
P 		29.1
(-2.6)
P 		53
B 70.8
(3)
P 		51.4
(-0.5)
P 		37.1
(-3)
P 		38.7
(-2.7)
P 		34
C 65.2
(1.5)
P 		53.8
(-0.1)
P 		75.7
(3)
P 		33.2
(-3)
P 		43
avr 71.5
(3) 		48.6
(-0.6) 		46.1
(-1) 		33.7
(-3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 159
1 2 3 4 5 6 range
A 45.9
(-2.3)
P 		66.7
(-0.9)
P 		36.1
(-3)
P 		46.1
(-2.3)
P 		122.9
(3)
P 		91.5
(0.8)
P 		87
B 114.4
(0.5)
P 		96.6
(-0.1)
P 		20.8
(-3)
P 		39.1
(-2.3)
P 		144.2
(1.7)
P 		180
(3)
P 		159
C 121.7
(3)
P 		77.7
(0.3)
P 		23.5
(-3)
P 		72.4
(-0)
P 		80.5
(0.5)
P 		98.6
(1.6)
P 		98
avr 94
(1.2) 		80.3
(0.3) 		26.8
(-3) 		52.5
(-1.4) 		115.9
(2.5) 		123.4
(3) 		97
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173663_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24700
Sequence Type Exemplar sequence
Representative Public ID C43062 NCBI
Target Description C43062 /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=T28F3.1 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C43062 /WB_GENE_ID=T28F3.1 /WP=CE19806 /CHR=4 /FEA=Genomic Cluster /DEF=C2 domain; C.elegans cDNA clone yk335h8 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070668(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070668 NCBI Caenorhabditis elegans T28F3.1a (T28F3.1) mRNA, complete cds. 11/11 None
T28F3.1a.1 ENSEMBL cdna:known chromosome:CEL140:IV:17281416:17284154:-1 gene:T28F3.1 11/11 None
T28F3.1a.2 ENSEMBL cdna:known chromosome:CEL140:IV:17281425:17284156:-1 gene:T28F3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
178508 2 1 NM_070668 173663_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:17281447-17284149(-) 100.0 100.0
Public Domain and Genome References
Ensembl T28F3.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05302 Hypothetical protein T28F3.1a [Caenorhabditis elegans] ref|NP_503069.1| T28F3.1a [Caenorhabditis elegans] sp|Q9XUB9|YGJI_CAEEL Hypothetical protein T28F3.1 in chromosome IV 0.0
blast CAD56248 Hypothetical protein T28F3.1b [Caenorhabditis elegans] ref|NP_872104.1| T28F3.1b [Caenorhabditis elegans] 0.0
blast CAE57422 Hypothetical protein CBG00380 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 9.1E-12
Pfam IPR000008 EMBL-EBI C2 domain 4.7E-11
Pfam IPR010734 EMBL-EBI Copine 2.4E-81
Sequence
Target Sequence 
>C. ELEGANS:173663_AT
ccgcctatgtattaattctaatcaaactattgtgtggttattagggaaactttgttataa
aagccaatttttgcctttttatatttctttcccaattatctcattcctcaacccctggca
taatattatttcgctttttaaaagctttccgatactcatcgtttctgaaattccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCGCCTATGTATTAATTCTAATCAA 238 263 1904 Antisense
ATTCTAATCAAACTATTGTGTGGTT 149 9 1918 Antisense
TGTGTGGTTATTAGGGAAACTTTGT 527 499 1934 Antisense
TGCCTTTTTATATTTCTTTCCCAAT 577 583 1975 Antisense
TATTTCTTTCCCAATTATCTCATTC 122 663 1985 Antisense
TTCCTCAACCCCTGGCATAATATTA 637 697 2007 Antisense
ACCCCTGGCATAATATTATTTCGCT 443 89 2014 Antisense
GCATAATATTATTTCGCTTTTTAAA 422 309 2021 Antisense
TTTTAAAAGCTTTCCGATACTCATC 478 673 2039 Antisense
GCTTTCCGATACTCATCGTTTCTGA 373 303 2047 Antisense
GATACTCATCGTTTCTGAAATTCCG 603 423 2054 Antisense
Sequence Source Affymetrix Proprietary Database