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[173654_at]

Description CEK019H6F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C42D8.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D35333 /WB_GENE_ID=C42D8.5 /WP=CE06951 /CHR=X /FEA=Genomic Cluster /DEF=peptidase; C.elegans cDNA clone yk19h6 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 07      6grp_high1_10_10 > 70      6grp_highest_nonorm > 43      6grp_total > 81      4grp_total > 57      6grp_highest_sub > 04      6grp_cgc > 51      6grp_high1_sub > 04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3123
1 2 3 4 range
A 79.2
(-2.8)
A 		1893.4
(1)
P 		2860.4
(3)
P 		7.7
(-3)
A 		2853
B 105
(-2.8)
A 		2271
(1.4)
P 		3128.5
(3)
P 		5.1
(-3)
A 		3123
C 91.5
(-2.9)
A 		2248.5
(2.3)
P 		2538.1
(3)
P 		33.6
(-3)
A 		2505
avr 91.9
(-2.8) 		2137.6
(1.5) 		2842.3
(3) 		15.5
(-3) 		2827

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1438
1 2 3 4 5 6 range
A 923.5
(-0.1)
P 		1506.3
(3)
P 		916.9
(-0.1)
P 		1420.6
(2.5)
P 		1283.8
(1.8)
P 		373.2
(-3)
P 		1133
B 1364.9
(1.2)
P 		1226.5
(0.6)
P 		1128.4
(0.2)
P 		1794.6
(3)
P 		942.6
(-0.6)
P 		356.8
(-3)
P 		1438
C 1101.1
(0.6)
P 		1026.5
(0.2)
P 		967.2
(-0.1)
P 		1382.3
(2.1)
P 		1548.7
(3)
P 		410
(-3)
P 		1139
avr 1129.8
(0.9) 		1253.1
(1.5) 		1004.2
(0.2) 		1532.5
(3) 		1258.4
(1.6) 		380
(-3) 		1153
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 884
1 2 3 4 range
A 28.8
(-2.8)
A 		411
(0.3)
P 		747.8
(3)
P 		1.1
(-3)
A 		747
B 33.5
(-2.7)
A 		560.9
(1.6)
P 		724.9
(3)
P 		1.1
(-3)
A 		724
C 30.3
(-2.8)
A 		646.5
(1.4)
P 		884.6
(3)
P 		5.7
(-3)
A 		879
avr 30.9
(-2.8) 		539.5
(1.1) 		785.8
(3) 		2.6
(-3) 		783

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 445
1 2 3 4 5 6 range
A 170.2
(-1.9)
P 		535.7
(3)
P 		199.7
(-1.5)
P 		360.3
(0.6)
P 		387.4
(1)
P 		90.9
(-3)
P 		445
B 474.9
(3)
P 		408.3
(1.8)
P 		274
(-0.5)
P 		475.3
(3)
P 		383
(1.4)
P 		133
(-3)
P 		342
C 408.1
(1.5)
P 		313.2
(-0.1)
P 		246.2
(-1.2)
P 		278
(-0.7)
P 		501.1
(3)
P 		137.3
(-3)
P 		364
avr 351.1
(1.6) 		419.1
(2.9) 		240
(-0.6) 		371.2
(2) 		423.8
(3) 		120.4
(-3) 		303
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173654_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25967
Sequence Type Exemplar sequence
Representative Public ID CEK019H6F NCBI
Target Description CEK019H6F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=C42D8.5 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D35333 /WB_GENE_ID=C42D8.5 /WP=CE06951 /CHR=X /FEA=Genomic Cluster /DEF=peptidase; C.elegans cDNA clone yk19h6 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029283(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029283 NCBI Caenorhabditis elegans ACE(angiotensin converting enzyme)-like Non-peptidase family member (acn-1) (acn-1) mRNA, complete cds. 11/11 None
C42D8.5b.2 ENSEMBL cdna:known chromosome:CEL140:X:5092157:5097040:-1 gene:C42D8.5 11/11 None
C42D8.5b.1 ENSEMBL cdna:known chromosome:CEL140:X:5092425:5097040:-1 gene:C42D8.5 11/11 None
C42D8.5b.3 ENSEMBL cdna:known chromosome:CEL140:X:5092425:5097034:-1 gene:C42D8.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000033887 4/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180780 2 1 NM_001029282 188527_s_at (11)
NM_001029283 173654_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5092471-5097036(-) 97.03 100.0
Public Domain and Genome References
Ensembl C42D8.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001024454 ACE(angiotensin converting enzyme)-like Non-peptidase family member (acn-1) [Caenorhabditis elegans] gb|AAU05584.1| Ace(angiotensin converting enzyme)-like non-peptidase protein 1, isoform b [Caenorhabditis elegans] 1.0E-143
blast NP_001024453 ACE(angiotensin converting enzyme)-like Non-peptidase family member (acn-1) [Caenorhabditis elegans] gb|AAA98719.2| Ace(angiotensin converting enzyme)-like non-peptidase protein 1, isoform a [Caenorhabditis elegans] sp|Q18581|ACN1_CAEEL Inactive angiotensin-converting enzyme-related protein precursor (ACE-like non-metallopeptidase protein 1) 2.0E-59
blast CAE68692 Hypothetical protein CBG14607 [Caenorhabditis briggsae] 4.0E-50
Sequence
Target Sequence 
>C. ELEGANS:173654_AT
tatgtttgtttcaaattttcccatttttcaaaaactcttttttttttagaaccttaggtt
tccctgacctgaaatctctgactcccccaatttgctgcaatctttattaattatttaatg
ataatttattgagtttcgggccgatgctacattagtcactattttgcattttgaatgtag
ctgctgatgaaaaacggttaaaagacttttatttcaatgtttttcttactttacctttct
tgtttttgtctctttccccccaataagtacacttatctcagttgtacagcata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATGTTTGTTTCAAATTTTCCCATT 191 657 2747 Antisense
TTAGGTTTCCCTGACCTGAAATCTC 624 265 2800 Antisense
TTTCCCTGACCTGAAATCTCTGACT 573 669 2805 Antisense
CTCCCCCAATTTGCTGCAATCTTTA 462 233 2828 Antisense
AATTTATTGAGTTTCGGGCCGATGC 521 173 2869 Antisense
GGGCCGATGCTACATTAGTCACTAT 365 491 2884 Antisense
ATTAGTCACTATTTTGCATTTTGAA 500 13 2897 Antisense
GCATTTTGAATGTAGCTGCTGATGA 291 307 2912 Antisense
GTAGCTGCTGATGAAAAACGGTTAA 113 457 2923 Antisense
TTCCCCCCAATAAGTACACTTATCT 641 695 3000 Antisense
ACACTTATCTCAGTTGTACAGCATA 66 111 3015 Antisense
Sequence Source Affymetrix Proprietary Database