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[173649_at]

Description CEK011F6F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F18A12.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D34718 /WB_GENE_ID=F18A12.8 /WP=CE23669 /CHR=2 /FEA=Genomic Cluster /DEF=peptidase; C.elegans cDNA clone yk11f6 : 5prime end, single read.
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 16      4grp_highest_8_8 > 53      4grp_high_8_8 > 18      6grp_highest_nonorm > 40      6grp_total > 06      4grp_total > 30      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1169
1 2 3 4 range
A 1595.3
(3)
P 		739.7
(-2.1)
P 		587.4
(-3)
P 		1452.7
(2.2)
P 		1008
B 1645
(3)
P 		742.5
(-1.6)
P 		476.3
(-3)
P 		1203
(0.7)
P 		1169
C 1477.6
(3)
P 		873.4
(-1.3)
P 		641.5
(-3)
P 		1286.4
(1.6)
P 		836
avr 1572.6
(3) 		785.2
(-1.7) 		568.4
(-3) 		1314
(1.5) 		1004

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 923
1 2 3 4 5 6 range
A 1254.1
(3)
P 		822.2
(-0.2)
P 		514.2
(-2.4)
P 		452.8
(-2.9)
P 		547.7
(-2.2)
P 		438.4
(-3)
P 		816
B 1350.1
(3)
P 		880
(-0.1)
P 		571.2
(-2.1)
P 		585.3
(-2)
P 		485
(-2.7)
P 		434.3
(-3)
P 		916
C 1026
(3)
P 		866.2
(1.4)
P 		598.9
(-1.3)
P 		496.1
(-2.3)
P 		462.3
(-2.6)
P 		427.1
(-3)
P 		599
avr 1210.1
(3) 		856.1
(0.3) 		561.4
(-2) 		511.4
(-2.4) 		498.3
(-2.5) 		433.3
(-3) 		777
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 470
1 2 3 4 range
A 580.8
(3)
P 		160.6
(-2.9)
P 		153.6
(-3)
P 		212.6
(-2.2)
P 		427
B 524.6
(3)
P 		183.4
(-1.9)
P 		110.4
(-3)
P 		256.4
(-0.9)
P 		414
C 489.1
(3)
P 		251.1
(-2.2)
P 		223.6
(-2.9)
P 		216.9
(-3)
P 		272
avr 531.5
(3) 		198.4
(-2.4) 		162.5
(-3) 		228.6
(-1.9) 		369

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 370
1 2 3 4 5 6 range
A 231.2
(1)
P 		292.4
(3)
P 		112
(-2.8)
P 		114.8
(-2.7)
P 		165.3
(-1.1)
P 		106.7
(-3)
P 		186
B 469.8
(3)
P 		292.9
(-0.2)
P 		138.7
(-3)
P 		155
(-2.7)
P 		197
(-1.9)
P 		162
(-2.6)
P 		331
C 380.3
(3)
P 		264.3
(0.5)
P 		152.4
(-1.9)
P 		99.8
(-3)
P 		149.6
(-1.9)
P 		143
(-2.1)
P 		281
avr 360.4
(3) 		283.2
(1) 		134.4
(-2.7) 		123.2
(-3) 		170.6
(-1.8) 		137.2
(-2.6) 		237
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173649_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23757
Sequence Type Exemplar sequence
Representative Public ID CEK011F6F NCBI
Target Description CEK011F6F /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F18A12.8 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D34718 /WB_GENE_ID=F18A12.8 /WP=CE23669 /CHR=2 /FEA=Genomic Cluster /DEF=peptidase; C.elegans cDNA clone yk11f6 : 5prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182128(11), NM_062137(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182128 NCBI Caenorhabditis elegans F18A12.8b (peptidase) mRNA, complete cds. 11/11 None
NM_062137 NCBI Caenorhabditis elegans F18A12.8a (peptidase) mRNA, complete cds. 11/11 None
F18A12.8b ENSEMBL cdna:known chromosome:CEL140:II:3420661:3425527:-1 gene:F18A12.8 11/11 A 
F18A12.8a ENSEMBL cdna:known chromosome:CEL140:II:3420661:3425527:-1 gene:F18A12.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3420672-3425527(-) 99.3 100.0
Public Domain and Genome References
Ensembl F18A12.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN65322 Hypothetical protein F18A12.8b [Caenorhabditis elegans] ref|NP_871928.1| F18A12.8b [Caenorhabditis elegans] 0.0
blast AAB66079 Hypothetical protein F18A12.8a [Caenorhabditis elegans] ref|NP_494538.1| F18A12.8a [Caenorhabditis elegans] 0.0
blast CAE70962 Hypothetical protein CBG17774 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR008753 EMBL-EBI Peptidase M13 8.9E-82
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 2.2E-65
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 4.1E-87
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_871928.1 1 75-97
NP_494538.1 1 75-97
F18A12.8b 1 75-97
F18A12.8a 1 75-97
Sequence
Target Sequence 
>C. ELEGANS:173649_AT
gtattgtttggtaatctgttcctttttttgtctgtgctccttatctcaagctctctttga
ttcttcctacataggcacaaaaatgctgcctgcctgcctaactaggttgcctgactaggc
aggcttgcaggcaggcatatttgtgcctctctcattagttcccaaagctgccttacgggt
ctctcccgtgaaaatgtacatatttccgattttataattctaccgtgtgactcttccctt
gaaaactcttctcgtctcgttttgacctacaaaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTATTGTTTGGTAATCTGTTCCTTT 593 451 2547 Antisense
TCCTTATCTCAAGCTCTCTTTGATT 53 593 2584 Antisense
TCTCTTTGATTCTTCCTACATAGGC 138 613 2598 Antisense
GGCACAAAAATGCTGCCTGCCTGCC 8 535 2620 Antisense
CCTGCCTAACTAGGTTGCCTGACTA 427 265 2639 Antisense
ACTAGGCAGGCTTGCAGGCAGGCAT 668 101 2660 Antisense
GGCAGGCATATTTGTGCCTCTCTCA 159 537 2676 Antisense
TTCCCAAAGCTGCCTTACGGGTCTC 420 673 2705 Antisense
GGGTCTCTCCCGTGAAAATGTACAT 166 497 2723 Antisense
TATAATTCTACCGTGTGACTCTTCC 378 659 2759 Antisense
CTCGTCTCGTTTTGACCTACAAAAT 195 233 2797 Antisense
Sequence Source Affymetrix Proprietary Database