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[173629_at]

Description CEK013H6R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F36A2.13 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D32411 /WB_GENE_ID=F36A2.13 /WP=CE24948 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk13h6 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 58      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 83      6grp_total > 48      4grp_total > 39      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 322
1 2 3 4 range
A 530
(3)
P 		356.2
(-1)
P 		270.2
(-3)
P 		365.8
(-0.8)
P 		260
B 533
(3)
P 		383.2
(-2.6)
P 		372
(-3)
P 		521.1
(2.6)
P 		161
C 592.2
(3)
P 		354.2
(-3)
P 		430.7
(-1.1)
P 		410.6
(-1.6)
P 		238
avr 551.7
(3) 		364.5
(-2.8) 		357.6
(-3) 		432.5
(-0.7) 		194

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 507
1 2 3 4 5 6 range
A 562
(1.9)
P 		385.2
(-1.8)
P 		327.7
(-3)
P 		380.2
(-1.9)
P 		614.6
(3)
P 		574.6
(2.2)
P 		287
B 560.9
(0)
P 		615.3
(0.7)
P 		335.4
(-2.6)
P 		608.1
(0.6)
P 		305.1
(-3)
P 		812.1
(3)
P 		507
C 499.5
(0.3)
P 		558.6
(3)
P 		427.9
(-3)
P 		429.6
(-2.9)
P 		476.2
(-0.8)
P 		463.1
(-1.4)
P 		131
avr 540.8
(1.2) 		519.7
(0.7) 		363.7
(-3) 		472.6
(-0.4) 		465.3
(-0.6) 		616.6
(3) 		253
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 143
1 2 3 4 range
A 193
(3)
P 		77.3
(-2)
P 		70.6
(-2.3)
P 		53.5
(-3)
P 		140
B 170
(3)
P 		94.6
(-2.4)
P 		86.2
(-3)
P 		111
(-1.2)
P 		84
C 196
(3)
P 		101.8
(-1.5)
P 		150.1
(0.8)
P 		69.2
(-3)
P 		127
avr 186.3
(3) 		91.2
(-2.3) 		102.3
(-1.6) 		77.9
(-3) 		108

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 231
1 2 3 4 5 6 range
A 103.6
(-1.3)
P 		137
(0.4)
P 		71.4
(-3)
P 		96.4
(-1.7)
P 		185.5
(3)
P 		139.9
(0.6)
P 		114
B 195.2
(0.1)
P 		204.8
(0.3)
P 		81.5
(-3)
P 		161.1
(-0.8)
P 		124
(-1.8)
P 		302.8
(3)
P 		221
C 185.2
(3)
P 		170.5
(2.1)
P 		108.9
(-1.6)
P 		86.4
(-3)
P 		154.1
(1.1)
P 		155
(1.2)
P 		99
avr 161.3
(1) 		170.8
(1.5) 		87.3
(-3) 		114.6
(-1.5) 		154.5
(0.6) 		199.2
(3) 		112
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173629_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23030
Sequence Type Exemplar sequence
Representative Public ID CEK013H6R NCBI
Target Description CEK013H6R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F36A2.13 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D32411 /WB_GENE_ID=F36A2.13 /WP=CE24948 /CHR=1 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk13h6 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059988(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059988 NCBI Caenorhabditis elegans F36A2.13 (F36A2.13) mRNA, complete cds. 11/11 None
F36A2.13 ENSEMBL cdna:known chromosome:CEL140:I:8836182:8846828:-1 gene:F36A2.13 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8836123-8846649(-) 98.02 100.0
Public Domain and Genome References
Gene Title
Ensembl F36A2.13
Entrez Gene 172697 Entrez gene
SwissProt O17858 EMBL-EBI
WormBase CE24948 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1591792_AT similar to Ubiquitin--protein ligase EDD (Hyperplastic discs protein homolog) (100 kDa protein) cfa
CANINE:1588332_AT similar to Ubiquitin--protein ligase EDD (Hyperplastic discs protein homolog) (100 kDa protein) cfa
CANINE_2:CFA.17328.1.S1_S_AT similar to Ubiquitin--protein ligase EDD (Hyperplastic discs protein homolog) (100 kDa protein) cfa
CANINE_2:CFAAFFX.1865.1.S1_AT similar to Ubiquitin--protein ligase EDD (Hyperplastic discs protein homolog) (100 kDa protein) cfa
CANINE_2:CFAAFFX.1869.1.S1_S_AT similar to Ubiquitin--protein ligase EDD (Hyperplastic discs protein homolog) (100 kDa protein) cfa
DROSOPHILA_2:1641297_AT hyperplastic discs dm
DROSGENOME1:143227_AT hyperplastic discs dm
CHICKEN:GGA.1293.1.S1_AT similar to KIAA0896 protein gga
CHICKEN:GGA.1293.3.S1_A_AT similar to KIAA0896 protein gga
CHICKEN:GGAAFFX.25297.1.S1_AT similar to KIAA0896 protein gga
HG-U133A_2:208882_S_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-FOCUS:208882_S_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U133_PLUS_2:208882_S_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U133A:208882_S_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U133A:208884_S_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U133_PLUS_2:208884_S_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U133A_2:208884_S_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HU35KSUBA:RC_AA621776_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HU35KSUBA:R70621_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HU35KSUBA:RC_AA489203_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HU35KSUBC:RC_W86992_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HU35KSUBD:RC_AA421714_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
U133_X3P:G4101694_3P_A_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
U133_X3P:HS.278428.0.A3_3P_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
U133_X3P:HS.278428.0.A2_3P_A_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U133A_2:208883_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U133_PLUS_2:208883_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U133A:208883_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U95AV2:39035_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U95AV2:39036_G_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U95B:45514_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U95C:49311_R_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U95C:57383_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U133_PLUS_2:232264_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U133B:232264_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U133_PLUS_2:1555888_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HU35KSUBA:AA382517_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
U133_X3P:HS2.382038.1.S1_3P_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
U133_X3P:HS2.278428.4.A1_3P_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
U133_X3P:HS.288719.0.S1_3P_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U133_PLUS_2:1568903_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U95E:70394_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
HG-U95E:85941_R_AT E3 ubiquitin protein ligase, HECT domain containing, 1 hs
MG-U74AV2:101413_AT E3 ubiquitin protein ligase, HECT domain containing, 1 mm
MOUSE430_2:1444327_AT E3 ubiquitin protein ligase, HECT domain containing, 1 mm
MOE430B:1444327_AT E3 ubiquitin protein ligase, HECT domain containing, 1 mm
MU11KSUBA:AA475847_S_AT E3 ubiquitin protein ligase, HECT domain containing, 1 mm
MU11KSUBB:MSA.7793.0_S_AT E3 ubiquitin protein ligase, HECT domain containing, 1 mm
MOE430A:1452718_AT E3 ubiquitin protein ligase, HECT domain containing, 1 mm
MOUSE430A_2:1452718_AT E3 ubiquitin protein ligase, HECT domain containing, 1 mm
MOUSE430_2:1452718_AT E3 ubiquitin protein ligase, HECT domain containing, 1 mm
MG-U74CV2:135931_AT Hyperplastic discs protein (Edd) mm
MOE430B:1441718_AT Hyperplastic discs protein (Edd) mm
MOUSE430_2:1441718_AT Hyperplastic discs protein (Edd) mm
MU19KSUBA:TC17841_AT Hyperplastic discs protein (Edd) mm
MU19KSUBB:TC28252_AT Hyperplastic discs protein (Edd) mm
MU19KSUBC:TC41030_AT Hyperplastic discs protein (Edd) mm
MU19KSUBC:TC41030_G_AT Hyperplastic discs protein (Edd) mm
RG-U34A:RC_AI237592_AT progestin induced protein rn
RAE230A:1367719_AT progestin induced protein rn
RAT230_2:1367719_AT progestin induced protein rn
RG-U34B:RC_AA997087_AT Progestin induced protein rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4842 ubiquitin-protein ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB82212 Hypothetical protein F36A2.13 [Caenorhabditis elegans] emb|CAB82204.1| Hypothetical protein F36A2.13 [Caenorhabditis elegans] ref|NP_492389.1| F36A2.13 [Caenorhabditis elegans] 0.0
blast T21546 hypothetical protein F36A2.13 - Caenorhabditis elegans 0.0
blast G87867 protein F36A2.13 [imported] - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 1.4E-4
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 5.5E-66
Pfam IPR003126 EMBL-EBI Zn-finger (putative), N-recognin 2.0E-26
Sequence
Target Sequence 
>C. ELEGANS:173629_AT
gtaaccatttttctatttcagcttttaattatctttttttttaactatttatatatttaa
catgttcttctccccacctgataaccctttcgttagttttttatatgtattccaggtttt
aaatagcgggaagctgctaaaatctcttctatttcaattttacccttgtttccaatttat
ttttttcaaaaaaccatcatgcttagttaggatcaactacactaaatctcaaacagcttt
ttcgcttcagtttgacaatgatttctggactcactcaccaatttcactttcgatttttga
gcgtaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAACCATTTTTCTATTTCAGCTTT 674 461 8854 Antisense
TATATTTAACATGTTCTTCTCCCCA 211 661 8905 Antisense
TCGTTAGTTTTTTATATGTATTCCA 497 603 8943 Antisense
ATCTCTTCTATTTCAATTTTACCCT 374 33 8995 Antisense
AAACCATCATGCTTAGTTAGGATCA 429 125 9044 Antisense
TAGGATCAACTACACTAAATCTCAA 567 653 9061 Antisense
CTAAATCTCAAACAGCTTTTTCGCT 226 223 9075 Antisense
AGCTTTTTCGCTTCAGTTTGACAAT 419 79 9088 Antisense
ACAATGATTTCTGGACTCACTCACC 702 113 9108 Antisense
CTCACTCACCAATTTCACTTTCGAT 9 229 9123 Antisense
TTCACTTTCGATTTTTGAGCGTAAT 316 687 9136 Antisense
Sequence Source Affymetrix Proprietary Database