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[173627_at]

Description CEK100EZR_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F54C8.7 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D66442 /WB_GENE_ID=F54C8.7 /WP=CE00193 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk100e12 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 90      6grp_high1_10_10 > 09      4grp_highest_8_8 > 09      4grp_high_8_8 > 62      6grp_highest_nonorm > 75      6grp_total > 03      4grp_total > 58      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 623
1 2 3 4 range
A 920.5
(-1.3)
P 		1260.6
(3)
P 		1229.4
(2.6)
P 		782.9
(-3)
P 		478
B 1032.7
(-0.3)
P 		1172.8
(1)
P 		1376.7
(3)
P 		753.3
(-3)
P 		623
C 1007.5
(-1.1)
P 		1171.3
(2)
P 		1227.7
(3)
P 		902.8
(-3)
P 		325
avr 986.9
(-0.8) 		1201.6
(2) 		1277.9
(3) 		813
(-3) 		465

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 422
1 2 3 4 5 6 range
A 1048.1
(1.8)
P 		985.5
(0.8)
P 		961.5
(0.4)
P 		1118.8
(3)
P 		1100.2
(2.7)
P 		758.6
(-3)
P 		360
B 1019.5
(0.9)
P 		920.1
(-2.8)
P 		994
(-0)
P 		1039.9
(1.6)
P 		1076.7
(3)
P 		913.8
(-3)
P 		163
C 1180.2
(3)
P 		1090.9
(1.4)
P 		891.6
(-2.2)
P 		850
(-3)
P 		978.3
(-0.7)
P 		925.7
(-1.6)
P 		330
avr 1082.6
(3) 		998.8
(0.7) 		949
(-0.7) 		1002.9
(0.8) 		1051.7
(2.1) 		866
(-3) 		217
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 313
1 2 3 4 range
A 335.1
(3)
P 		273.6
(1.3)
P 		321.4
(2.6)
P 		114.6
(-3)
P 		221
B 329.3
(3)
P 		289.6
(1.6)
P 		319
(2.6)
P 		160.5
(-3)
P 		169
C 333.5
(0.9)
P 		336.8
(1)
P 		427.9
(3)
P 		152.2
(-3)
P 		276
avr 332.6
(2.3) 		300
(1.4) 		356.1
(3) 		142.4
(-3) 		214

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 267
1 2 3 4 5 6 range
A 193.2
(-2.7)
P 		350.5
(3)
P 		209.4
(-2.1)
P 		283.7
(0.6)
P 		332
(2.3)
P 		184.7
(-3)
P 		166
B 354.7
(0.5)
P 		306.3
(-1)
P 		241.4
(-3)
P 		275.4
(-2)
P 		437.4
(3)
P 		340.7
(0)
P 		196
C 437.4
(3)
P 		332.9
(0.6)
P 		226.9
(-1.7)
P 		170.9
(-3)
P 		316.6
(0.3)
P 		309.9
(0.1)
P 		267
avr 328.4
(1.5) 		329.9
(1.6) 		225.9
(-3) 		243.3
(-2.2) 		362
(3) 		278.4
(-0.7) 		136
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173627_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.23923
Sequence Type Exemplar sequence
Representative Public ID CEK100EZR NCBI
Target Description CEK100EZR_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=F54C8.7 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D66442 /WB_GENE_ID=F54C8.7 /WP=CE00193 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk100e12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027433(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027433 NCBI Caenorhabditis elegans F54C8.7a (F54C8.7) mRNA, complete cds. 11/11 None
F54C8.7a ENSEMBL cdna:known chromosome:CEL140:III:9451494:9452909:-1 gene:F54C8.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176326 2 1 NM_001027433 173627_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9451517-9452906(-) 95.9 100.0
Public Domain and Genome References
Ensembl F54C8.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA80157 Hypothetical protein F54C8.7a [Caenorhabditis elegans] ref|NP_001022604.1| F54C8.7a [Caenorhabditis elegans] sp|P34445|YL87_CAEEL Hypothetical protein F54C8.7 in chromosome III 1.0E-146
blast CAE65214 Hypothetical protein CBG10089 [Caenorhabditis briggsae] 1.0E-128
blast CAD82913 Hypothetical protein F54C8.7b [Caenorhabditis elegans] ref|NP_871661.1| F54C8.7b [Caenorhabditis elegans] 1.0E-125
Protein Domains
Database ID Description E-Value
Pfam IPR010504 EMBL-EBI Arfaptin-like 9.6E-117
Sequence
Target Sequence 
>C. ELEGANS:173627_AT
attccaaccatgtgatttcattgtatgatattcctaacattcctgccttattcctatgtc
actttttttctttttttcccgaattattgcttttgaccgggtttgcaagacactgctgcc
aggtttttttctgtgtataatagacgaactaggggatttttaaaaattataagtaatgtt
ctaattttatgtgtctttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCCAACCATGTGATTTCATTGTA 551 7 929 Antisense
ATTGTATGATATTCCTAACATTCCT 492 3 948 Antisense
TGCCTTATTCCTATGTCACTTTTTT 297 585 972 Antisense
TATTCCTATGTCACTTTTTTTCTTT 496 663 977 Antisense
TTTCCCGAATTATTGCTTTTGACCG 473 669 1003 Antisense
GAATTATTGCTTTTGACCGGGTTTG 22 323 1009 Antisense
GACCGGGTTTGCAAGACACTGCTGC 366 383 1023 Antisense
GTTTGCAAGACACTGCTGCCAGGTT 620 451 1029 Antisense
GACACTGCTGCCAGGTTTTTTTCTG 252 363 1037 Antisense
GCTGCCAGGTTTTTTTCTGTGTATA 165 297 1043 Antisense
AATGTTCTAATTTTATGTGTCTTTT 18 165 1103 Antisense
Sequence Source Affymetrix Proprietary Database