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[173616_at]

Description CEK004F5R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=K11D12.10 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D27173 /WB_GENE_ID=K11D12.10 /WP=CE25944 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk4f5 : 3prime end, single read.
Cluster 6grp_highest_10_10 > 43      6grp_high1_10_10 > 34      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 71      6grp_total > 02      4grp_total > 31      6grp_highest_sub > 02      6grp_cgc > 52      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 299
1 2 3 4 range
A 482.7
(3)
P 		184
(-3)
P 		243.9
(-1.8)
P 		356.1
(0.5)
P 		299
B 422.5
(3)
P 		366.8
(0.9)
P 		259.8
(-3)
P 		402.2
(2.3)
P 		163
C 457.7
(3)
P 		317.3
(-3)
P 		352.5
(-1.5)
P 		345.5
(-1.8)
P 		140
avr 454.3
(3) 		289.4
(-2.9) 		285.4
(-3) 		367.9
(-0.1) 		169

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 204
1 2 3 4 5 6 range
A 265.6
(-3)
P 		415.6
(2.5)
P 		344.8
(-0.1)
P 		273.9
(-2.7)
P 		428.9
(3)
P 		311.5
(-1.3)
P 		163
B 431.2
(1.7)
P 		457
(2.8)
P 		326.7
(-2.7)
P 		462.2
(3)
P 		319.3
(-3)
P 		336.7
(-2.3)
P 		143
C 423.3
(0.9)
P 		469.3
(3)
P 		348.8
(-2.4)
P 		375.5
(-1.2)
P 		358.1
(-2)
P 		336.2
(-3)
P 		133
avr 373.4
(-0.7) 		447.3
(3) 		340.1
(-2.4) 		370.5
(-0.9) 		368.8
(-1) 		328.1
(-3) 		119
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 175.8
(3)
P 		39.9
(-3)
P 		63.8
(-1.9)
P 		52.1
(-2.5)
P 		136
B 134.7
(3)
P 		90.6
(-0.6)
P 		60.2
(-3)
P 		85.7
(-0.9)
P 		75
C 151.5
(3)
P 		91.2
(-0.9)
P 		122.9
(1.2)
P 		58.3
(-3)
P 		93
avr 154
(3) 		73.9
(-2.4) 		82.3
(-1.9) 		65.4
(-3) 		89

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 49
(-3)
P 		147.8
(3)
P 		75.1
(-1.4)
P 		69.5
(-1.8)
P 		129.4
(1.9)
P 		75.8
(-1.4)
P 		99
B 150
(2.8)
P 		152.1
(3)
P 		79.3
(-3)
P 		122.4
(0.6)
P 		129.7
(1.2)
P 		125.6
(0.8)
P 		73
C 156.9
(3)
P 		143.2
(2)
P 		88.8
(-2)
P 		75.5
(-3)
P 		115.9
(-0)
P 		112.6
(-0.3)
P 		81
avr 118.6
(0.4) 		147.7
(3) 		81.1
(-3) 		89.1
(-2.3) 		125
(1) 		104.7
(-0.9) 		67
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 173616_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.25709
Sequence Type Exemplar sequence
Representative Public ID CEK004F5R NCBI
Target Description CEK004F5R_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=K11D12.10 /5_PRIME_EXT_DB=WormBase Gene ID /GB=D27173 /WB_GENE_ID=K11D12.10 /WP=CE25944 /CHR=5 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk4f5 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171456(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171456 NCBI Caenorhabditis elegans human MLK (Mixed Lineage Kinase) homolog family member (mlk-1) (mlk-1) mRNA, complete cds. 11/11 None
K11D12.10b ENSEMBL cdna:known chromosome:CEL140:V:5056209:5064590:-1 gene:K11D12.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
178895 2 1 NM_171456 173616_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5056223-5064591(-) 100.0 100.0
Public Domain and Genome References
Ensembl K11D12.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_741536 human MLK (Mixed Lineage Kinase) homolog family member (mlk-1) [Caenorhabditis elegans] gb|AAM15589.1| Human mlk (mixed lineage kinase) homolog protein 1, isoform b [Caenorhabditis elegans] 0.0
blast NP_741537 human MLK (Mixed Lineage Kinase) homolog family member (mlk-1) [Caenorhabditis elegans] gb|AAM15588.1| Human mlk (mixed lineage kinase) homolog protein 1, isoform a [Caenorhabditis elegans] 0.0
blast CAE64061 Hypothetical protein CBG08663 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001452 EMBL-EBI SH3 7.0E-15
Pfam IPR000719 EMBL-EBI Protein kinase 7.8E-53
Sequence
Target Sequence 
>C. ELEGANS:173616_AT
gaatcccagcatcttcgtaattaacgatgctattgtacatttctcaagttttatcctctt
ttcttttttacgcacttcatgtgttttcaaacaatttctttattttctccgagttagtta
tatgtgtcaatttttcctcctcaatccatacattccgtgagtatctttgttaccatagct
cttcctatttttgtctcttttctcttcaaaacgtgtacaaacccatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATCCCAGCATCTTCGTAATTAAC 681 329 2676 Antisense
AACGATGCTATTGTACATTTCTCAA 400 145 2698 Antisense
CATTTCTCAAGTTTTATCCTCTTTT 518 215 2713 Antisense
CTTTTCTTTTTTACGCACTTCATGT 52 223 2733 Antisense
TACGCACTTCATGTGTTTTCAAACA 58 647 2744 Antisense
ATTTTCTCCGAGTTAGTTATATGTG 100 15 2777 Antisense
TATATGTGTCAATTTTTCCTCCTCA 532 661 2794 Antisense
CAATCCATACATTCCGTGAGTATCT 469 181 2817 Antisense
GTATCTTTGTTACCATAGCTCTTCC 290 455 2836 Antisense
ATAGCTCTTCCTATTTTTGTCTCTT 342 23 2850 Antisense
TCTTCAAAACGTGTACAAACCCATT 442 613 2878 Antisense
Sequence Source Affymetrix Proprietary Database