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[173615_s_at]

Description C57056_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=Y111B2A.7 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C57056 /WB_GENE_ID=Y111B2A.7 /WP=CE26635 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk279b12 : 3prime end, single read.
Cluster 6grp_high1_10_10 > 78      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_total > 68      4grp_total > 47      6grp_high1_sub > Cu      6grp_total_sub > Increase      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 196
1 2 3 4 range
A 290.6
(3)
P 		128
(-2.3)
P 		139.5
(-2)
P 		107.9
(-3)
P 		183
B 228.7
(3)
P 		152.4
(-0.4)
P 		95.1
(-3)
P 		106.8
(-2.5)
P 		134
C 239.1
(3)
P 		115.2
(-2.8)
P 		111.6
(-3)
P 		145.2
(-1.4)
P 		128
avr 252.8
(3) 		131.9
(-2.3) 		115.4
(-3) 		120
(-2.8) 		137

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 137
1 2 3 4 5 6 range
A 103.2
(3)
P 		68.1
(-1.2)
P 		52.5
(-3)
P 		63.9
(-1.7)
A 		58.9
(-2.2)
A 		71.3
(-0.8)
P 		51
B 114.7
(-0.5)
P 		65
(-3)
P 		104.8
(-1)
P 		69.6
(-2.8)
P 		88.7
(-1.8)
P 		186.1
(3)
P 		121
C 49.6
(-3)
P 		84.2
(0.6)
P 		92.5
(1.4)
P 		50.4
(-2.9)
A 		107.5
(3)
A 		96.9
(1.9)
P 		58
avr 89.2
(-0.1) 		72.4
(-1.8) 		83.3
(-0.7) 		61.3
(-3) 		85
(-0.5) 		118.1
(3) 		57
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 105.8
(3)
P 		27.8
(-2.2)
P 		36.5
(-1.6)
P 		15.8
(-3)
P 		90
B 72.9
(3)
P 		37.7
(-1.1)
P 		22
(-3)
P 		22.8
(-2.9)
P 		51
C 79.2
(3)
P 		33.1
(-2.1)
P 		38.9
(-1.4)
P 		24.5
(-3)
P 		55
avr 86
(3) 		32.9
(-1.9) 		32.5
(-1.9) 		21
(-3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 19
(0.6)
P 		24.2
(3)
P 		11.4
(-3)
P 		16.2
(-0.8)
A 		17.8
(0)
A 		17.4
(-0.2)
P 		13
B 39.9
(-0.5)
P 		21.6
(-2.6)
P 		25.4
(-2.2)
P 		18.4
(-3)
P 		36
(-0.9)
P 		69.4
(3)
P 		51
C 18.4
(-1)
P 		25.7
(0.8)
P 		23.6
(0.3)
P 		10.1
(-3)
A 		34.8
(3)
A 		32.5
(2.4)
P 		25
avr 25.8
(-0.4) 		23.8
(-0.9) 		20.1
(-1.7) 		14.9
(-3) 		29.5
(0.5) 		39.8
(3) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 173615_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.24150
Sequence Type Exemplar sequence
Representative Public ID C57056 NCBI
Target Description C57056_rc /REP_DB=TREMBL Accession /5_PRIME_EXT_ID=Y111B2A.7 /5_PRIME_EXT_DB=WormBase Gene ID /GB=C57056 /WB_GENE_ID=Y111B2A.7 /WP=CE26635 /CHR=3 /FEA=Genomic Cluster /DEF=C.elegans cDNA clone yk279b12 : 3prime end, single read.
Cluster Evidence This cluster is supported by a Genomic Cluster.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067236(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067236 NCBI Caenorhabditis elegans Y111B2A.8 (Y111B2A.8) mRNA, complete cds. 10/11 None
GENEFINDER00000006281 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:12538879:12547813:-1 10/11 None
SNAP00000006152 ENSEMBL cdna:SNAP chromosome:CEL140:III:12547208:12547813:-1 11/11 None
Y111B2A.8 ENSEMBL cdna:known chromosome:CEL140:III:12538863:12547956:-1 gene:Y111B2A.8 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:12547089-12547810(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y111B2A.8
Entrez Gene 176677 Entrez gene
SwissProt Q9BHL6 EMBL-EBI
WormBase CE34111 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264625_AT protein kinase, putative at
ATGENOME1:17666_AT protein kinase, putative at
CANINE:1587769_S_AT similar to 5-AMP-activated protein kinase, gamma-1 subunit (AMPK gamma-1 chain) (AMPKg) cfa
CANINE_2:CFA.3131.1.A1_AT similar to 5-AMP-activated protein kinase, gamma-1 subunit (AMPK gamma-1 chain) (AMPKg) cfa
CANINE_2:CFAAFFX.13848.1.S1_AT similar to 5-AMP-activated protein kinase, gamma-1 subunit (AMPK gamma-1 chain) (AMPKg) cfa
DROSOPHILA_2:1626570_S_AT SNF4/AMP-activated protein kinase gamma subunit dm
DROSGENOME1:154116_AT SNF4/AMP-activated protein kinase gamma subunit dm
DROSGENOME1:150301_AT SNF4/AMP-activated protein kinase gamma subunit dm
HG-U133_PLUS_2:201805_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit hs
HG-U133A:201805_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit hs
HG-FOCUS:201805_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit hs
HG-U133A_2:201805_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit hs
HUGENEFL:U42412_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit hs
U133_X3P:G4506060_3P_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit hs
HG-U95AV2:34346_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit hs
HU35KSUBA:AA481308_AT Protein kinase, AMP-activated, gamma 1 non-catalytic subunit hs
HG-U95D:90049_AT Protein kinase, AMP-activated, gamma 1 non-catalytic subunit hs
HU35KSUBC:RC_F03010_AT Protein kinase, AMP-activated, gamma 1 non-catalytic subunit hs
MU11KSUBA:AA266932_S_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit mm
MU11KSUBB:MSA.5541.0_S_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit mm
MU11KSUBB:MSA.30968.0_S_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit mm
MG-U74AV2:100632_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit mm
MOE430A:1417690_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit mm
MOUSE430A_2:1417690_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit mm
MOUSE430_2:1417690_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit mm
MOE430A:1433533_X_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit mm
MOUSE430A_2:1433533_X_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit mm
MOUSE430_2:1433533_X_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit mm
RG-U34A:U42413_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit rn
RAE230A:1367947_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit rn
RAT230_2:1367947_AT protein kinase, AMP-activated, gamma 1 non-catalytic subunit rn
YEAST_2:1776708_AT Protein kinase activator found in a complex containing Snf1p and members of the Sip1p/Sip2p/Gal83p family; activates the Snf1p protein kinase; involved in expression of glucose-repressed genes, sporulation, and peroxisome biogenesis Sc
Protein Similarities
Method ID Description E-Value
blast CAC35836 Hypothetical protein Y111B2A.8 [Caenorhabditis elegans] ref|NP_499637.2| Y111B2A.8 [Caenorhabditis elegans] 0.0
blast CAE66414 Hypothetical protein CBG11682 [Caenorhabditis briggsae] 1.0E-162
blast NP_998326 AMP-activated protein kinase subunit gamma 1 [Danio rerio] gb|AAH59181.1| Zgc:63896 [Danio rerio] 6.0E-93
blast NP_998326 AMP-activated protein kinase subunit gamma 1 [Danio rerio] gb|AAH59181.1| Zgc:63896 [Danio rerio] 1.0E-92
blast CAC35836 Hypothetical protein Y111B2A.8 [Caenorhabditis elegans] ref|NP_499637.2| Y111B2A.8 [Caenorhabditis elegans] 6.0E-92
Protein Domains
Database ID Description E-Value
Pfam IPR000644 EMBL-EBI CBS domain 5.1E-5
Pfam IPR000644 EMBL-EBI CBS domain 5.5E-11
Pfam IPR000644 EMBL-EBI CBS domain 3.4E-10
Pfam IPR000644 EMBL-EBI CBS domain 6.5E-10
Pfam IPR000644 EMBL-EBI CBS domain 5.1E-5
Pfam IPR000644 EMBL-EBI CBS domain 5.5E-11
Pfam IPR000644 EMBL-EBI CBS domain 3.4E-10
Pfam IPR000644 EMBL-EBI CBS domain 6.5E-10
Sequence
Target Sequence 
>C. ELEGANS:173615_S_AT
aagagcaaggcgtctggaacccttcgtcggacacttttcggacggagcacgaaacgacga
gccaaagatgctgaagcgattcatgaagccggggaggaggaggaggatgaagaggattat
acacaggataactatgatacagtgaaatcgttgccagcttcaggacattatggaaaatcg
cgagtgggtaacacgtcgcacacgaagcttcaagatttagctgatgagatgaggcttcga
ggaggtggaccaccgaaacaaggatccgagcctccgatcccaaaacctccggtaataatc
ttccgtgtacctcgcaagcatgcgaattcaatgtcatcactacgtgtctcaccgggagca
attgggccatcgggtgttgtcaaggcgccgctttccgatccgaactttgacttttacacg
tggcacacgtctgaagcgggttag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGAGCAAGGCGTCTGGAACCCTTC 338 153 176 Antisense
ATCGTTGCCAGCTTCAGGACATTAT 519 35 322 Antisense
GTAACACGTCGCACACGAAGCTTCA 609 461 363 Antisense
TGAGGCTTCGAGGAGGTGGACCACC 465 565 405 Antisense
GACCACCGAAACAAGGATCCGAGCC 650 383 423 Antisense
TTCCGTGTACCTCGCAAGCATGCGA 221 699 476 Antisense
CAATGTCATCACTACGTGTCTCACC 461 183 504 Antisense
TCTCACCGGGAGCAATTGGGCCATC 498 609 522 Antisense
CATCGGGTGTTGTCAAGGCGCCGCT 135 211 543 Antisense
GAACTTTGACTTTTACACGTGGCAC 602 349 577 Antisense
GTGGCACACGTCTGAAGCGGGTTAG 423 477 595 Antisense
Sequence Source Affymetrix Proprietary Database